Analysis of the electrophoretic karyotype ofFlammulina velutipes

Assignment of RAPD marker probes designed from 12 linkage groups of Flammulina velutipes to CHEF-separated chromosomal DNAs

“…Although Southern hybridization analysis was not performed on monokaryons, the haploid genome sizes could be calculated by weighting doublet or triplet chromosomal DNA bands ranging from 7 to 10 (9) chromosomes as 24.1-36.4 Mb in omFSB1 and 23.0-31.7 Mb in omFSB2 (Table 1). This size was 1.0-1.8 times greater than both the result obtained by simple summing of the sizes of separated DNAs here and that previously reported for this fungus (Kim et al 2000;Tanesaka et al 2003;Park et al 2010). …”

“…By this method, electrophoretic karyotypes have been revealed for many fungi including cultivated edible mushrooms such as A. bisporus (Royer et al 1991(Royer et al , 1992Sonnenberg et al 1991;Lodder et al 1993), L. edodes (Arima and Morinaga 1993), Pleurotus species (Sagawa and Nagata 1992;Tamai et al 1995), and F. velutipes (Kim et al 2000;Tanesaka et al 2003;Park et al 2010). Various reports have suggested that F. velutipes has at least six (Kim et al 2000), or six or seven (Park et al 2010), or seven or eight (Tanesaka et al 2003) haploid chromosomes. Chromosome-specific probes have also been constructed by assignment of DNA probes prepared from a cDNA library to CHEF-separated chromosomes (Kim et al 2000;Park et al 2010).…”

“…Various reports have suggested that F. velutipes has at least six (Kim et al 2000), or six or seven (Park et al 2010), or seven or eight (Tanesaka et al 2003) haploid chromosomes. Chromosome-specific probes have also been constructed by assignment of DNA probes prepared from a cDNA library to CHEF-separated chromosomes (Kim et al 2000;Park et al 2010). On the other hand, assignment using DNA probes prepared from a genetic linkage map is more effective to elucidate the karyotype involved in haploid chromosome number and genome size and for analysis of meiotic behavior, as demonstrated in A. bisporus (Royer et al 1991(Royer et al , 1992Kerrigan et al 1993;Sonnenberg et al 1996).…”

“…No probes hybridized to the smallest two chromosomes, CB 10 and 11 (Table 1). Kim et al (2000) and Park et al (2010) assigned several DNA probes prepared from a cDNA library to CHEFseparated F. velutipes chromosomes using Southern hybridization analysis. This analysis was powerful enough to construct chromosome-specific probes and also enabled detection of weak bands on gels.…”

Electrophoretic karyotype of Flammulina velutipes and its variation among monokaryotic progenies

“…(Royer et al 1992;Kerrigan et al 1993), Lentinula edodes (Berk.) Pegler (Arima and Morinaga 1993), Pleurotus species (Sagawa and Nagata 1992;Tamai et al 1995), and F. velutipes (Kim et al 2000).…”