Analysis of the entire nucleotide sequence of hepatitis B virus: characteristics of HBeAg-positive asymptomatic carriers, HBeAb positive asymptomatic carriers and patients with liver cirrhosis

Hong, Soon-Kwang; Niitsuma, Hiroaki; Nagasaki, Futoshi; Cervantes, Julieta G.; Ojima, Toshiaki; Ueno, Yoshiyuki; Kobayashi, Koju; Ishii, Motoyasu; Shimosegawa, Tooru

doi:10.1016/s1386-6346(01)00185-1

Cited by 2 publications

(1 citation statement)

References 13 publications

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“…We used each set of sequencing primers previously described [Shan et al, 2002]. Direct sequencing of the PCR products was carried out by a fluorescence autosequencer (model 377, PE Japan Applied Biosystems, Chiba, Japan) using a Big Dye Terminator Sequencing Kit (PE Japan Applied Biosystems) according to the manufacturer's instructions.…”

Section: Nucleotide Sequences Of Hbv Isolatesmentioning

confidence: 99%

Analysis of the entire nucleotide sequence of hepatitis B causing consecutive cases of fatal fulminant hepatitis in Miyagi Prefecture Japan

Nagasaki

Ueno

Niitsuma

et al. 2008

Journal of Medical Virology

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We encountered five consecutive patients with fulminant hepatitis induced by acute hepatitis B virus (HBV) infection in 2000--2001 in Japan. They had not had previous contact each other, and were referred to us from different hospitals. Although a 69-year-old woman could be rescued by intensive internal treatment, the four patients died. We analyzed the partial (nt 278-646) and entire nucleotide sequences of the HBV obtained from them, and their divergences were 0-0.3% and 0-0.2%, respectively. The results suggested that they had been infected with the same HBV isolates. The isolates belonged to genotype B and subgenotype B2 on the phylogenetic tree analysis (AB302942-AB302946). As for the nucleotides sequences of them, previously reported mutations of G1896A, A1762T, and G1764A were present. Amino acid analysis revealed that previously reported Ile97Leu and Pro130Non-Pro in the core region and Trp28Stop in the precore region were present. As for the entire nucleotide sequences among B2, AB302942 showed low divergences with AF121245 and AB073834 (1.7%), and X97850 from patients with fulminant hepatitis (3.2%). We compared the two consensus nucleotides derived from AB302942 and X97850 (fulminant hepatitis) versus AY121245 and AB073834 (non-fulminant hepatitis), which revealed a difference in nt 1,504 located in the P and X region. Nucleotide 1,504 was C for isolates from fulminant hepatitis and G for non-fulminant hepatitis, and it was recognized among most of the isolates belonging to B2 registered on GenBank. Further studies could disclose the mechanism of severe inflammation of liver that finally leads to fulminant hepatitis.

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Section: Nucleotide Sequences Of Hbv Isolatesmentioning

confidence: 99%

Analysis of the entire nucleotide sequence of hepatitis B causing consecutive cases of fatal fulminant hepatitis in Miyagi Prefecture Japan

Nagasaki

Ueno

Niitsuma

et al. 2008

Journal of Medical Virology

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Analysis of the entire nucleotide sequence of hepatitis B virus genotype B in the Philippines reveals a new subgenotype of genotype B

Nagasaki

Niitsuma

Cervantes

et al. 2006

Journal of General Virology

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The entire nucleotide sequences were determined for hepatitis B virus (HBV) genotype B (HBV/B) genomes extracted from five patients in the Philippines and designated GenBank AB219426, AB219427, AB219428, AB219429 and AB219430. The serotype of the first four isolates was ayw and that of GenBank AB219430 was adw. Divergences of entire sequences were 1?0-2?0 % between the first four isolates and 3?8-4?2 % between these four and GenBank AB219430. Phylogenetic-tree analysis revealed that, worldwide, HBV/B comprises five subgenotypes: B1, B2, B3, B4 and the new Philippines group, designated B5. Divergences of the entire genome sequences between four isolates in subgenotype B5 and isolates from other countries (subgenotypes) were 4?4-4?8 % with Vietnam (B4), 2?9-3?5 % with Indonesia (B3), 4?7-5?1 % with China (B2) and 5?4-6?0 % with Japan (B1). Similarly, GenBank AB219430 showed the lowest divergences: 3?4 % with the isolate from Indonesia (B3), 5?0 % with Vietnam (B4), 5?4 % with China (B2) and 6?1 % with Japan (B1). This is the first report of entire nucleotide sequences of HBV/B from the Philippines and the results show that these sequences belong to a new subgenotype, B5. The present study identified that HBV/B isolates throughout the world are divided genetically into five subgenotypes, the relationships between geographical distances and the genetic distances of HBV/B being well-correlated.

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Analysis of the entire nucleotide sequence of hepatitis B virus: characteristics of HBeAg-positive asymptomatic carriers, HBeAb positive asymptomatic carriers and patients with liver cirrhosis

Cited by 2 publications

References 13 publications

Analysis of the entire nucleotide sequence of hepatitis B causing consecutive cases of fatal fulminant hepatitis in Miyagi Prefecture Japan

Analysis of the entire nucleotide sequence of hepatitis B causing consecutive cases of fatal fulminant hepatitis in Miyagi Prefecture Japan

Analysis of the entire nucleotide sequence of hepatitis B virus genotype B in the Philippines reveals a new subgenotype of genotype B

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