Analysis of the expression and clinical significance of miR-382-5P in ovarian cancer based on biological information

Abstract

Objective : To investigate the expression, potential function and clinical significance of miR-382-5P in ovarian cancer tissues . Results: The expression of miR-382-5P is tissue-specific. Four target gene prediction softwares including miRPathDB ,DIANA ,miRWalk and starBase simultaneously predicted 24 target genes (AHRR，ANKS1A，C15orf41，CABYR，CASP3，COBLL1，EEF2K，FBXO28，FGFR1OP，LRP12，MDM4，PAIP2，PARM1，PDE5A，PIAS2，SAR1B，SCD，SLC44A1，TIMM17A，TSPYL1，UBXN7， YES1，ZBTB37，ZNF587). Significant enrichment of target gene function in biological processes, cell composition and molecular functions, signal transduction pathways are significantly enriched in microRNAs in cancer , MAPK signaling pathway, Hepatitis B, FoxO signaling pathway, Non-small cell lung cancer, apoptosis, Colorectal cancer, Oxytocin signaling pathway. TCGA database data analysis did not indicate that there was a correlation between the expression of miR-382-5P and the clinicopathological parameters of ovarian cancer, but there was no significant difference (P>0.05). The results of QRT-PCR in 72 ovarian cancer clinical specimens showed that the expression of miR-382-5P was significantly increased in drug-resistant tissues (4.60±2.959) and significantly decreased in sensitive tissues (1.88±2.082). The difference was statistically significant (P<0.05). Analyzing the follow-up data of 72 patients with ovarian cancer in our hospital, we found that the expression level of miR-382-5P in ovarian cancer patients was correlated with the degree of drug resistance, and the difference was statistically significant (P<0.05). The expression level of miR-382-5P, drug resistance , recurrence and treatment response in patients with ovarian cancer were correlated with their overall survival (OS), and the difference was statistically significant (P<0.05). The expression level of miR-382-5P, drug resistance, treatment response, recurrence and residual lesion size in patients with ovarian cancer were correlated with their progression-free survival (PFS), the difference was statistically significant (P<0.05). Multidrug resistance is an independent risk factor affecting the prognosis of ovarian cancer, the difference was statistically significant (P<0.05) Conclusion ： After comprehensive analysis, it is found that miR-382-5P may become a new target for the treatment of multidrug resistant ovarian cancer. The signal pathways of MAPK, Fox-1,apoptosis and so on may be the main mechanism of miR-382-5P mediating the occurrence and development of ovarian cancer, and lay a certain foundation for the research of targeted miR-382-5P in the treatment of multidrug resistant ovarian cancer.