Analysis of the Expression of Neurotrophins and Their Receptors in Adult Zebrafish Kidney

Abstract

Neurotrophins and their receptors are involved in the development and maintenance of neuronal populations. Different reports have shown that all neurotrophin/receptor pathways can also play a role in several non-neuronal tissues in vertebrates, including the kidney. These signaling pathways are involved in different events to ensure the correct functioning of the kidney, such as growth, differentiation, and regulation of renal tubule transport. Previous studies in some fish species have identified the neurotrophins and receptors in the kidney. In this study, for the first time, we compare the expression profiles (mRNA and protein) of all neurotrophin/receptor pathways in the kidney of the adult zebrafish. We quantify the levels of mRNA by using qPCR and identify the expression pattern of each neurotrophin/receptor pathway by in situ hybridization. Next, we detect the proteins using Western blotting and immunohistochemistry. Our results show that among all neurotrophins analyzed, NT-3/TrkC is the most expressed in the glomerule and tubule and in the hematopoietic cells, similar to what has been reported in the mammalian kidney.

Transcription Pattern of Neurotrophic Factors and Their Receptors in Adult Zebrafish Spinal Cord

“…In the transverse section of spinal cord, two regions are clearly distinct, central and peripheral. In adult zebrafish, neurotrophins and their receptors were extensively described in different tissues and organs: the brain, kidney, ovary, testis, and the sensory and olfactory organs [ 32 , 33 , 34 , 35 , 36 , 37 , 38 , 39 , 40 , 41 ]. However, there are no extensive studies regarding the specific identification and quantification of the mRNA localization pattern of neurotrophins and their receptors in the adult zebrafish spinal cord.…”