Analysis of the factors influencing red clover (Trifolium pratense L., Fabaceae) isoflavone content

Sazdanić, Darija; Mikulić, Marina; Kladar, Nebojša; Jelena, Hogervorst; Milica, Atanacković Krstonošić

doi:10.5281/zenodo.2452290

Cited by 3 publications

(4 citation statements)

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“…More specifically, samples originating from Lithuania and Serbia belonged to the same group, while samples originating from Brazil and Finland were both separated. It was pointed out that ecological characteristics of habitat, with an emphasis on temperature and precipitation, are influencing factors on the content of these secondary metabolites [ 46 ]. Also, it is likely that varieties with a lower isoflavone content were created through selection in order to prevent disruption of the reproductive cycle in sheep caused by a high concentration of isoflavones.…”

Section: Discussionmentioning

confidence: 99%

“…Namely, it was previously established that the isoflavone content can be influenced by periods of sunlight, current weather conditions, soil characteristics and many other abiotic factors [4]. For example, in a paper published by Sazdanić et al (2018), statistical analysis showed that red clover samples grown in different countries are grouped based on isoflavone content. More specifically, samples originating from Lithuania and Serbia belonged to the same group, while samples originating from Brazil and Finland were both separated.…”

Section: Discussionmentioning

confidence: 99%

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Phytochemical Composition of Different Red Clover Genotypes Based on Plant Part and Genetic Traits

Mikulić,

Atanacković Krstonošić,

Kladar

et al. 2023

Foods

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Red clover (Trifolium pratense L.) is an important legume that is also known as a rich source of isoflavones, which are compounds with mild estrogenic activity. Therefore, this plant is often used as a raw material in the production of dietary supplements recommended in menopause. Many factors can influence isoflavone content, but those genetically related are considered to be the most important. Therefore, the aim of this study was to evaluate the phytochemical profile of different plant parts of 30 red clover genotypes grouped according to ploidy and country of seed origin by analyzing the content of dominant isoflavones, total phenolic content (TPC) and antioxidant activity. It was found that there are significant differences in the examined traits among plant parts. Red clover leaves had the highest total isoflavone content, with biochanin A as the dominant compound, while flower extracts had the highest TPC and antioxidant activity. Diploid and tetraploid genotypes were significantly different concerning the content of daidzein, genistein, formononetin and TPC with higher quantities in tetraploid samples. On the other hand, seed origin was not a useful separating factor for the analyzed samples. The results of this research indicate that ploidy, as a previously poorly studied factor, could influence isoflavone content in red clover.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Phytochemical Composition of Different Red Clover Genotypes Based on Plant Part and Genetic Traits

Mikulić,

Atanacković Krstonošić,

Kladar

et al. 2023

Foods

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show abstract

“…On the other hand, daidzein and genistein, represent only a small portion of the total isoflavone concentration. 9,10 The Trifolium pratense L. extracts have been used in menopause as a natural and safety form of hormone replacement therapy because of their high content of estrogenic isoflavones such as daidzein, genistein, formononetin, and biochanin A. 13 These isoflavones are estrogenic in vitro and in vivo, but little information exists their effects on the endometrium and inflamed decidua.…”

Section: Introductionmentioning

confidence: 99%

“…7,8 The isoflavones are found in physiologically significant amounts in the Trifolium pratense L. extracts. 9,10 Trifolium pratense L. is a well-known source of phytoestrogenic isoflavones. It contains mainly formononetin and biochanin A, and much smaller amounts of daidzein and genistein, mostly in the glycosidic form.…”

Section: Introductionmentioning

confidence: 99%

The Effects of the Trifolium Pratense L. Extract on the Leukemia Inhibitory Factor and its Receptor in The Endometrial Epithelial Cell Line

Köktürk

Dağıstanlı

Doğan

et al. 2021

Natural Product Communications

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Isoflavones have attracted much notice due to their health advantages; however, a comprehensive understanding of the effects of isoflavones on endometrium biology remains undiscovered. The expression and deficiency of leukemia inhibitory factor (LIF) and LIF receptor (LIFR) has been shown to be involved in multiple implantations failures in female infertility. Mechanisms implicated in the failure of implantations require further researches, thus our aim is to investigate the effect of the Trifolium pratense L. isoflavone extract with abundant formononetin content on implantation through assessing LIF and LIFR expressions. The Ishikawa cells were cultured with 20, 30, and 40 µg/mL concentrations of Trifolium pratense L. isoflavone extracts for 24 h and detected staining intensity of LIF and LIFR by immunocytochemistry and immunofluorescence staining using image analysis software. As compared with the control and 20 µg/mL Trifolium pratense L. groups, the staining intensity of LIF and LIFR in 30 and 40 µg/mL Trifolium pratense L. groups were significantly increased ( P < .0001). Our findings suggest that Trifolium pratense L. isoflavone extract may alter the endometrium expression of LIF and LIFR in the human endometrial adenocarcinoma cell line.

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Short-Term and Long-Term Effects of Plasma and Radio Wave Seed Treatments on Red Clover Plants

Nedved

Kalatskaja

Kopylova

et al. 2021

IOP Conf. Ser.: Earth Environ. Sci.

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The effect of high-frequency 5.28 MHz plasma and radio wave seed treatments on red clover growth, nodule formation and isoflavonoid content has been investigated. The short-term and long-term effects of plasma and radio wave treatment of seeds were evaluated on clover plants during two years of vegetation. The response of plants to plasma treatment was increment in root growth and the nodule formation. Significant changes in the length and weight of roots were noted in the first year of vegetation. The increase in isoflavonoids content observed after plasma and radio wave treatments can be explained by the advancement in nodulation process.

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Analysis of the factors influencing red clover (Trifolium pratense L., Fabaceae) isoflavone content

Cited by 3 publications

References 0 publications

Phytochemical Composition of Different Red Clover Genotypes Based on Plant Part and Genetic Traits

Phytochemical Composition of Different Red Clover Genotypes Based on Plant Part and Genetic Traits

The Effects of the Trifolium Pratense L. Extract on the Leukemia Inhibitory Factor and its Receptor in The Endometrial Epithelial Cell Line

Short-Term and Long-Term Effects of Plasma and Radio Wave Seed Treatments on Red Clover Plants

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