Analysis of the gene family encoding the Entamoeba histolytica galactose-specific adhesin 170-kDa subunit

Purdy, Jay E.; Mann, Barbara J.; Shugart, Erika C.; Petri, William A.

doi:10.1016/0166-6851(93)90177-y

Cited by 37 publications

(17 citation statements)

References 11 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The heavy subunit of the Gal-GalNAc adhesin is encoded by five genes in E. histolytica strain HM1:IMSS (83,121,155). The derived amino acid sequences suggest that the heavy subunit is an integral membrane protein with a small cytoplasmic tail and a large extracellular N-terminal domain containing a cysteinerich region.…”

Section: Early Invasive Lesions With Superficial Ulcerationmentioning

confidence: 99%

Pathogenesis of Intestinal Amebiasis: From Molecules to Disease

Espinosa‐Cantellano¹,

Martı́nez-Palomo²

2000

Clinical Microbiology Reviews

191

115

View full text Add to dashboard Cite

Section: Early Invasive Lesions With Superficial Ulcerationmentioning

confidence: 99%

Pathogenesis of Intestinal Amebiasis: From Molecules to Disease

Espinosa‐Cantellano¹,

Martı́nez-Palomo²

2000

Clinical Microbiology Reviews

191

115

View full text Add to dashboard Cite

“…Three genes encoding the 170 kDa heavy subunit and two encoding the 31-35 kDa light subunit have been cloned and sequenced (Mann et al, 1991;Tannich et al , 1991Tannich et al , , 1992McCoy et al, 1993;Purdy et al, 1993). The 170 kDa subunit has a transmembrane domain whereas the 31 kDa subunit isoform but not the 35 kDa isoform is glycophosphatidylinosito1 (GP1)-linked (McCoy et al, 1993).…”

Section: Gavgalnac-binding Lectinmentioning

confidence: 99%

Glycobiology of Parasites: Role of Carbohydrate–Binding Proteins and Their Ligands in the Host–Parasite Interaction

Ward¹

1996

Glycosciences

View full text Add to dashboard Cite

“…Each lectin subunit is represented in the E. histolytica genome by multiple alleles; however, the functional distinctions between these alleles have not yet been established. Six genes with 53 to 85% identity encode Lgl isoforms, and at least two genes encoding specific Hgl isoforms (Hgl-2 and Hgl-3) have been isolated and well studied (15,19). Furthermore, another Hgl isoform and an incomplete sequence have been described from the genome (10).…”

mentioning

confidence: 99%

Stress by Heat Shock Induces Massive Down Regulation of Genes and Allows Differential Allelic Expression of the Gal/GalNAc Lectin in Entamoeba histolytica

et al. 2006

View full text Add to dashboard Cite

Gene expression analysis by microarray assay revealed that when exposed to stress, Entamoeba histolytica exhibits a specific heat shock response, together with a dramatic overall reduction in gene transcription as well as differential allelic expression of key genes participating in virulence, such as the galactose/N-acetylgalactosamine (Gal/GalNAc) lectin.Amoebiasis is a disease caused by the enteric protozoan parasite Entamoeba histolytica. Following invasion of human tissue by E. histolytica, the two major clinical manifestations are hemorrhagic colitis and liver abscesses (18). For infection to succeed, invading trophozoites must produce an adaptive response that ensures their protection against the host response and survival. Hence, E. histolytica proteins whose production is triggered or modulated by environmental stress are of great interest, since characterization of these species should help us understand the mechanisms which sustain pathogenesis and could lead to new treatments for amoebiasis.Microbial pathogens have evolved a number of strategies for protecting themselves from their hosts. One of these is the so-called heat shock response, which is elicited by a sudden increase in ambient temperature (13) and induces the synthesis of a limited set of proteins (called heat shock proteins [HSPs] or molecular chaperones). Homologues of known HSPs have been identified and partially characterized in E. histolytica (1,11). With the aim of determining gene expression changes during E. histolytica's adaptive response during infection, we developed an oligonucleotidebased microarray with transcript information randomly obtained from a cultured virulent strain of the pathogen. Array analysis revealed that gene transcription in E. histolytica exposed to heat shock is dramatically reduced, since 471 of 1,131 unique genes were down regulated, whereas specific HSP-encoding genes were up regulated. In conjunction with real-time PCR results, these genetic information data reveal for the first time a very interesting differential allelic expression of key genes participating in virulence, such as the immunodominant antigen Gal/GalNAc lectin, certain cysteine proteinases, and the so-called 20-kDa antigen.The aims of this study were (i) to establish a highly discriminating method for monitoring gene expression changes in E. histolytica and (ii) to determine the mRNA expression profile of E. histolytica cells growing in a drastically modified environment. We decided to construct an oligonucleotide-based microarray, using information obtained directly from sequence analysis of E. histolytica transcripts, a strategy that is generally thought to overcome problems due to gene redundancy and the presence of introns. A cDNA library of the virulent E. histolytica strain HM-1:IMSS and a liver-specific cDNA subtraction library were prepared and sequenced. The bioinformatic analysis of sequenced clones enabled us to define 1,300 bona fide transcripts, all from parasites growing in vitro and enriched with randomly chosen transcrip...

show abstract

Analysis of the gene family encoding the Entamoeba histolytica galactose-specific adhesin 170-kDa subunit

Cited by 37 publications

References 11 publications

Pathogenesis of Intestinal Amebiasis: From Molecules to Disease

Pathogenesis of Intestinal Amebiasis: From Molecules to Disease

Glycobiology of Parasites: Role of Carbohydrate–Binding Proteins and Their Ligands in the Host–Parasite Interaction

Stress by Heat Shock Induces Massive Down Regulation of Genes and Allows Differential Allelic Expression of the Gal/GalNAc Lectin in Entamoeba histolytica

Contact Info

Product

Resources

About