Analysis of the genetic distribution among members of Clostridium botulinum group I using a novel multilocus sequence typing (MLST) assay

Olsen, Jaran Strand; Scholz, Holger C.; Fillo, Silvia; Ramisse, Vincent; Lista, Florigio; Trømborg, Anette K.; Aarskaug, Tone; Thrane, Ingjerd; Blatny, Janet Martha

doi:10.1016/j.mimet.2013.11.003

Cited by 22 publications

(18 citation statements)

References 35 publications

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“…Currently, there are 13 finished genomes and 21 WGS genomic sequences available in GenBank. The availability of genomic sequence information has spawned the opportunity to use other molecular techniques such as such as multi-locus sequence typing (MLST) [73,89,88,99], variable number tandem repeat (VNTR) [89,90,42,132], and single nucleotide polymorphism (SNP) analyses [51] to characterize C. botulinum strains. Analysis of MLST sequences of housekeeping genes can differentiate strains within a Group or species, as can VNTR and SNP analyses.…”

Section: Mlst Vntr Snp Analysesmentioning

confidence: 99%

Historical and current perspectives on Clostridium botulinum diversity

Smith

Hill

Raphael

2015

Research in Microbiology

132

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Section: Mlst Vntr Snp Analysesmentioning

confidence: 99%

Historical and current perspectives on Clostridium botulinum diversity

Smith

Hill

Raphael

2015

Research in Microbiology

132

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“…These have included comparative genomic indexing (using a DNA microarray), multi-locus sequence typing (MLST), pulsed-field gel electrophoresis (PFGE), multi-locus variable number tandem repeat analysis (MVLA), amplified fragment length polymorphism (AFLP), and comparisons of core genome single nucleotide polymorphisms (SNPs) following whole genome sequencing [2, 5, 6•, 7•, 22, 43•, 44, 45, 46, 47]. These approaches typically separate strains in up to about ten lineages or clusters (depending on strains tested and criterion applied).…”

Section: Genomic Diversity Of Clostridium Botulinum Group Imentioning

confidence: 99%

Impact of Clostridium botulinum genomic diversity on food safety

Peck

Vliet

2016

Current Opinion in Food Science

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“…The nontoxigenic counterpart for group I C. botulinum is considered to be C. sporogenes (12,13). Studies investigating the genetic relationship of C. sporogenes and C. botulinum that employed molecular techniques such as multiple-locus variablenumber tandem-repeat analysis (MLVA) (14,15), DNA microarrays and analysis of the flagellar glycosylation island (FGI) (16), DNA-rRNA hybridization (17), 16S rRNA gene sequencing (18,19), and multilocus sequence typing (MLST) (20,21) have been reported and included other strains of C. sporogenes, but not PA 3679. A DNA-DNA hybridization study by Lee and Riemann (22) revealed that PA 3679 was 100% homologous to C. botulinum strain 62A, yet in a similar study, PA 3679 shared only 83% homology to C. botulinum strain A190 and 68% homology with C. sporogenes strain J-53 (23).…”

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confidence: 99%

Genetic Diversity of Clostridium sporogenes PA 3679 Isolates Obtained from Different Sources as Resolved by Pulsed-Field Gel Electrophoresis and High-Throughput Sequencing

Schill

Wang

Butler

et al. 2016

Appl Environ Microbiol

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bClostridium sporogenes PA 3679 is a nonpathogenic, nontoxic model organism for proteolytic Clostridium botulinum used in the validation of conventional thermal food processes due to its ability to produce highly heat-resistant endospores. Because of its public safety importance, the uncertain taxonomic classification and genetic diversity of PA 3679 are concerns. Therefore, isolates of C. sporogenes PA 3679 were obtained from various sources and characterized using pulsed-field gel electrophoresis (PFGE) and whole-genome sequencing. The phylogenetic relatedness and genetic variability were assessed based on 16S rRNA gene sequencing and whole-genome single nucleotide polymorphism (SNP) analysis. All C. sporogenes PA 3679 isolates were categorized into two clades (clade I containing ATCC 7955 NCA3679 isolates 1961-2, 1990, and 2007 and clade II containing PA 3679 isolates NFL, UW, FDA, and Campbell and ATCC 7955 NCA3679 isolate 1961-4). The 16S maximum likelihood (ML) tree clustered both clades within proteolytic C. botulinum strains, with clade I forming a distinct cluster with other C. sporogenes non-PA 3679 strains. SNP analysis revealed that clade I isolates were more similar to the genomic reference PA 3679 (NCTC8594) genome (GenBank accession number AGAH00000000.1) than clade II isolates were. The genomic reference C. sporogenes PA 3679 (NCTC8594) genome and clade I C. sporogenes isolates were genetically distinct from those obtained from other sources (University of Wisconsin, National Food Laboratory, U.S. Food and Drug Administration, and Campbell's Soup Company). Thermal destruction studies revealed that clade I isolates were more sensitive to high temperature than clade II isolates were. Considering the widespread use of C. sporogenes PA 3679 and its genetic information in numerous studies, the accurate identification and genetic characterization of C. sporogenes PA 3679 are of critical importance. F ood-borne botulism is a neuroparalytic disease that results from the ingestion of botulinum neurotoxin (BoNT) produced by clostridia, including Clostridium botulinum and rare strains of Clostridium baratii and Clostridium butyricum. C. botulinum is a heterogeneous species comprised of four distinct groups (groups I to IV) of strains that differ based on genetic and phenotypic properties (1). Group I (proteolytic) C. botulinum strains can also be uniquely differentiated from the other groups based on their ability to form highly heat-resistant endospores. Spores of this organism are ubiquitously found in the environment and are generally presumed to be a natural contaminant of foods that come in contact with the soil (2). The geographical distribution of C. botulinum varies by serotype. For example, in the United States, spores of C. botulinum strains producing type B toxin are typically found in the soils east of the Mississippi River, and type A strains are found in the western region of the United States. Serotype E strains are commonly found in the soil sediments of the Great Lakes regions and in Alaska...

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Analysis of the genetic distribution among members of Clostridium botulinum group I using a novel multilocus sequence typing (MLST) assay

Cited by 22 publications

References 35 publications

Historical and current perspectives on Clostridium botulinum diversity

Historical and current perspectives on Clostridium botulinum diversity

Impact of Clostridium botulinum genomic diversity on food safety

Genetic Diversity of Clostridium sporogenes PA 3679 Isolates Obtained from Different Sources as Resolved by Pulsed-Field Gel Electrophoresis and High-Throughput Sequencing

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