Analysis of the genetic diversity of ovine herpesvirus 2 in samples from livestock with malignant catarrhal fever

Russell, George C.; Scholes, S. F. E.; Twomey, D. F.; Courtenay, A. E.; Grant, Dawn M.; Lamond, Bruce; Norris, David C.; Willoughby, Kim; Haig, David M.; Stewart, James P.

doi:10.1016/j.vetmic.2014.04.011

Cited by 13 publications

(21 citation statements)

References 29 publications

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“…The top hit was interleukin-4 ( p = 3.2x10 -5 ), with similarity extending over the majority of the mature polypeptide (residues 26 to 151 of the alignment). This is in accord with previous suggestions that the A9.5 and Ov9.5 proteins share structural similarities with four-helix-bundle cytokines, in particular IL-4 [ 24 ] [ 25 ].…”

Section: Resultssupporting

confidence: 93%

“…To study possible functional consequences of sequence diversity, the A9.5 protein sequences were compared with previously established Ov9.5 sequences [ 25 ]. Phylogenetic analysis indicated that the A9.5 alleles were less diverse than the Ov9.5 alleles (compare branch lengths in Fig 3 ).…”

Section: Resultsmentioning

confidence: 99%

“…Each set of assays was controlled by the inclusion of known AlHV-1 positive and negative bovine genomic DNA samples and potential cross-contamination between reactions was controlled by the inclusion of template-free reactions. To study viral variation in positive samples, segments of the ORF50 gene, encoding the lytic cycle trans-activator protein, and of the spliced A9.5 gene [ 24 ] were sequenced and analysed as described previously for OvHV-2 [ 25 ]. ORF50 encodes a transcription factor expected to be highly conserved, whilst A9.5 encodes a predicted glycoprotein of unknown function that has been demonstrated to be polymorphic in both AlHV-1 and OvHV-2 [ 25 ],[ 24 ].…”

Section: Methodsmentioning

confidence: 99%

“…The novel spliced gene A9.5 is predicted to encode a secreted glycoprotein that has no homologue outside the MCF viruses [ 24 ]. A9.5 does have a detectable homologue in OvHV-2 (Ov9.5) that has been shown to be both polymorphic and highly variable with nine alleles showing as little as 50% predicted amino acid sequence identity [ 25 ]. So far, only two alleles of A9.5 have been identified: one (A9.5*0101] [ 24 ] in the C500 strain of AlHV-1, that has been passaged in vivo in the UK for almost 50 years [ 26 ], and the other (A9.5*0201) [ 27 ] in a diagnostic sample taken from an Ankole cow with MCF.…”

Section: Introductionmentioning

confidence: 99%

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Alcelaphine Herpesvirus-1 (Malignant Catarrhal Fever Virus) in Wildebeest Placenta: Genetic Variation of ORF50 and A9.5 Alleles

et al. 2015

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Alcelaphine herpesvirus–1 (AlHV-1), a causative agent of malignant catarrhal fever in cattle, was detected in wildebeest (Connochaetes taurinus) placenta tissue for the first time. Although viral load was low, the finding of viral DNA in over 50% of 94 samples tested lends support to the possibility that placental tissue could play a role in disease transmission and that wildebeest calves are infected in utero. Two viral loci were sequenced to examine variation among virus samples obtained from wildebeest and cattle: the ORF50 gene, encoding the lytic cycle transactivator protein, and the A9.5 gene, encoding a novel polymorphic viral glycoprotein. ORF50 was well conserved with six newly discovered alleles differing at only one or two base positions. In contrast, while only three new A9.5 alleles were discovered, these differed by up to 13% at the nucleotide level and up to 20% at the amino acid level. Structural homology searching performed with the additional A9.5 sequences determined in this study adds power to recent analysis identifying the four-helix bundle cytokine interleukin-4 (IL4) as the major homologue. The majority of MCF virus samples obtained from Tanzanian cattle and wildebeest encoded A9.5 polypeptides identical to the previously characterized A9.5 allele present in the laboratory maintained AlHV-1 C500 strain. This supports the view that AlHV-1 C500 is suitable for the development of a vaccine for wildebeest-associated MCF.

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Section: Resultssupporting

confidence: 93%

Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Alcelaphine Herpesvirus-1 (Malignant Catarrhal Fever Virus) in Wildebeest Placenta: Genetic Variation of ORF50 and A9.5 Alleles

et al. 2015

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“…Zazwyczaj MCF objawia się gorączką, utratą apetytu, wzmożoną produkcją wydzieliny z nosa i oczu, zmętnieniem rogówki, zapaleniem spojówek, krwistymi biegunkami, ataksją oraz powstawaniem zmian skórnych. W zależności od gatunku zakażonego zwierzęcia, śmierć następuje po kilku dniach od wystąpienia objawów, a typowe uszkodzenia obejmują liczne stany zapalne błon śluzowych różnych narządów (zwłaszcza jelit, pęcherza moczowego i płuc), zapalenie tętnic oraz stłuszczenie kłębuszkowe (14,69,75). Śmiertelność wynosi od 50% do 70%, zakażone zwierzęta zwykle padają w ciągu 48 godzin od wystąpienia pierwszych objawów (69,95).…”

Section: Zakażenia Herpeswirusowe Zwierząt Gospodarskich I Towarzysząunclassified

Herpesviral infections of farm and companion animals

Brzezicka¹,

Cymerys²,

Słońska³

et al. 2018

Medycyna Weterynaryjna

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Herpesviridae is a large family of DNA viruses, capable of infecting higher as well as lower vertebrates. To date, more than one hundred species have been isolated and identified, and new species are still being discovered. For many years mammalian herpesviruses have been of interest to researchers because of their prevalence and pathogenicity, as well as significant economic losses associated with herpesviral infections, especially in livestock.In the course of their evolution, herpesviruses have perfectly adapted to their hosts and have developed the ability to establish latency. For years, many diverse efforts have been made to eliminate herpesviral infections, but the vaccines produced are generally ineffective and do not provide protection against the establishment of latency. Therefore, further research on their pathogenesis and continuous monitoring are needed to prevent the occurrence and spread of herpesvirus infections, particularly in farm and companion animals.

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Histophilus somni disease conditions with simultaneous infections by ovine gammaherpesvirus 2 in cattle herds from Southern Brazil

et al. 2023

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Analysis of the genetic diversity of ovine herpesvirus 2 in samples from livestock with malignant catarrhal fever

Cited by 13 publications

References 29 publications

Alcelaphine Herpesvirus-1 (Malignant Catarrhal Fever Virus) in Wildebeest Placenta: Genetic Variation of ORF50 and A9.5 Alleles

Alcelaphine Herpesvirus-1 (Malignant Catarrhal Fever Virus) in Wildebeest Placenta: Genetic Variation of ORF50 and A9.5 Alleles

Herpesviral infections of farm and companion animals

Histophilus somni disease conditions with simultaneous infections by ovine gammaherpesvirus 2 in cattle herds from Southern Brazil

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