Analysis of the genetic diversity of wild, Spanish populations of the species<i>Elymus caninus</i>(L.) Linnaeus and<i>Elymus hispanicus</i>(Boiss.) Talavera by PCR-based markers and endosperm proteins

Nieto-López, Rosa Marìa; Casanova, Carlos; Soler, Consuelo

doi:10.1051/agro:2000166

Cited by 5 publications

(4 citation statements)

References 21 publications

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“…The mean value and the coefficient of variation (CV) of the Jaccard similarity indices for each pair of individuals were used to estimate the genetic diversity within each population. The CV can be considered a global indicator of the genetic variability independently of the number of genotypes studied (Nieto-Lopez et al 2000). Genetic variability resulted maximal for R2X, intermediate for R4X and minimal for C4X (Table 2).…”

Section: Resultsmentioning

confidence: 99%

Genetic diversity in sexual diploid and apomictic tetraploid populations of Paspalum notatum situated in sympatry or allopatry

Daurelio

Espinoza

Quarín

et al. 2004

Plant Systematics and Evolution

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Paspalum notatum is a subtropical grass widely distributed in the temperate areas of America. Diploids are sexual while polyploids give rise to clonal seeds through aposporous apomixis. RAPD markers were used to analyze the genetic structure of three natural populations: i) diploids reproducing sexually (R2X); ii) sympatric apomictic tetraploids collected in the vicinity of the diploids (R4X); iii) allopatric apomictic tetraploids growing in isolation (C4X). The apomictic reproduction rate was evaluated by the use of molecular markers in progeny tests, while chromosome-counting allowed the verification of ploidy levels. Data revealed that the R4X group presented a variation considerably higher than that observed for C4X. Jaccard ´s coefficients were used to produce a cluster diagram using the UPGMA method. All but one tetraploid genotypes grouped together and were associated to diploid genotype A21. The possibility of occasional generation of novel tetraploid clones from the interaction between tetraploid and diploid individuals is discussed.

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Section: Resultsmentioning

confidence: 99%

Genetic diversity in sexual diploid and apomictic tetraploid populations of Paspalum notatum situated in sympatry or allopatry

Daurelio

Espinoza

Quarín

et al. 2004

Plant Systematics and Evolution

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“…, ) corroborates this hypothesis. Surprisingly, E. hispanicus has been reported to be tetraploid (Soler et al, ) and/or hexaploid (Nieto‐López et al, ; García et al, ), whereas all samples examined in the present study were found to be octoploid (Data S1; Figs. , ).…”

Section: Discussionmentioning

confidence: 48%

“…Because E. hispanicus is a wild relative of some crop cereals, several studies have focused on the genetic diversity of the species (Soler et al, ; Nieto‐López et al, ; García et al, ). Based on similarities with E. caninus relative to polymorphisms of Random Amplification of Polymorphic DNA (RAPDs), Sequence‐Tagged Sites (STSs), and endosperm proteins, these authors have suggested that E. hispanicus probably combines the H and the St genomes.…”

Section: Discussionmentioning

confidence: 99%

“…Although assuming a genomic composition for E. marginatus containing the H and the St genomes, Löve () offered no cytogenetic evidence. Regarding E. hispanicus , several authors (Soler et al, ; Nieto‐López et al, ; García et al, ) have suggested the H and St genomes as the most likely genomic combination for the species, based on the molecular similarities with E. caninus , but the exact genomic formula has not been experimentally confirmed.…”

Section: Introductionmentioning

confidence: 99%

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Discovery of the genus Pseudoroegneria (Triticeae, Poaceae) in the Western Mediterranean on exploring the generic boundaries of Elymus

Lucía

Martínez‐Ortega

Rico

et al. 2018

J of Sytematics Evolution

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In this study, we review the classification of two species, Elymus hispanicus and E. marginatus, which are restricted to highly valuable and sensitive Mediterranean ecosystems. The genomic composition of the two species is analysed by in situ hybridization. In addition, lodicule morphology and foliar anatomy of both species are compared with those of E. caninus, E. repens, E. sibiricus (i.e., the type species of Elymus s.s.) and Pseudoroegneria strigosa (i.e., the type species of Pseudoroegneria). The genomic formula 2n = 8x = 56; HStStSt is proposed for E. hispanicus and 2n = 4x = 28; StSt for E. marginatus. In this latter species, the absence of the ribosomal genes in one of the two St genomes suggests that diploidization may have occurred during the evolution of the species. Regarding foliar anatomy, E. hispanicus, E. caninus, E. repens, and E. sibiricus shared several characteristics, but the leaf blades of E. marginatus proved anatomically more similar to those of Ps. strigosa. The data compiled support the contention that: (i) E. hispanicus belongs to Elymus s.s.; (ii) E. marginatus should be transferred to Pseudoroegneria; and (iii) the morphology of the lodicules should be carefully reconsidered for appropriately describing the boundaries between Elymus s.s. and Pseudoroegneria. The new combination Ps. marginata is proposed and a detailed iconography of the plant is provided.

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Prolamin storage proteins and alloploidy in wild populations of the small grass Brachypodium distachyon (L.) P. Beauv.

et al. 2011

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This paper reports the glutenin diversity of a collection of 23 wild populations of the grass Brachypodium distachyon collected in the Mediterranean and southern areas of the Iberian Peninsula. The plant material studied included the three different cytotypes of this species: 2n = 10, 2n = 20 and 2n = 30. A specific method of extraction was used to isolate the glutenin subunits from the caryopsis. Separation by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) showed them to correspond to wheat low-molecular-weight glutenin subunits (LMW-GS). Twenty-two LMW-GS-like were identified that showed great diversity within and between populations. All the populations investigated were polymorphic for the endosperm proteins studied. The 2n = 30 forms had the largest number of subunits; these were also more diverse than those of the 2n = 10 or 2n = 20 forms. The 2n = 10 forms, the most common in the higher, interior areas of the Iberian Peninsula, showed the smallest subunit variation. In fact, negative correlations were found between subunit diversity and altitude and longitude. In contrast, a positive correlation was detected with the annual average temperature and the indices of thermicity and Mediterraneity. The similarity between the populations was estimated using the Sorensen-Dice coefficient, calculated on the basis of the presence/absence of the 22 LMW-GS proteins. The similarity indices were used to produce a dendrogram using the unweighted pair-group method with arithmetic means (UPGMA). This method produced three main groups corresponding to the three cytotypes. An analysis was made of the possible correlation between eco-geographic and climatic factors and the gene diversity and polymorphism shown in the populations. The diversity correlated positively with the number of chromosomes (2n), annual mean temperature, index of thermicity and index of Mediterraneity. In contrast, diversity correlated negatively with altitude, longitude and index of precipitation during the summer. The number of chromosomes correlated negatively with altitude, longitude and precipitation during summer and positively with all the other climatic indices. These results are coherent with the fact that diploid forms were more common in areas of the interior and at higher altitude, where the climate is more extreme.

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Analysis of the genetic diversity of wild, Spanish populations of the speciesElymus caninus(L.) Linnaeus andElymus hispanicus(Boiss.) Talavera by PCR-based markers and endosperm proteins

Cited by 5 publications

References 21 publications

Genetic diversity in sexual diploid and apomictic tetraploid populations of Paspalum notatum situated in sympatry or allopatry

Genetic diversity in sexual diploid and apomictic tetraploid populations of Paspalum notatum situated in sympatry or allopatry

Discovery of the genus Pseudoroegneria (Triticeae, Poaceae) in the Western Mediterranean on exploring the generic boundaries of Elymus

Prolamin storage proteins and alloploidy in wild populations of the small grass Brachypodium distachyon (L.) P. Beauv.

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