Analysis of the genetic diversity and relationships among and within species of Hippophae (Elaeagnaceae) based on RAPD markers

Sheng, Hongmei; An, Lizhe; Chen, T.; Su, Xu; Liu, G. X.; Zheng, X. L.; Pu, Lili; Liu, Y. J.; Lian, YW

doi:10.1007/s00606-006-0413-1

Cited by 36 publications

(27 citation statements)

References 18 publications

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“…In contrast, molecular markers are uniquely advantageous because they are not affected by the environment and are powerful tools in cultivar characterization. Although DNA-based molecular markers have been utilized in genetic studies, cultivar characterization and identification of sea buckthorn have not been conducted (Sheng et al, 2006). Information regarding genetic diversity levels and the separation of the plant individuals studied have not been reported, and no single report has identified a large number of sea buckthorn cultivars or developed methods that may be useful in future studies.…”

Section: Introductionmentioning

confidence: 99%

A new strategy for complete identification of sea buckthorn cultivars by using random amplified polymorphic DNA markers

Yang

Ding

et al. 2015

Genet. Mol. Res.

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ABSTRACT. DNA fingerprinting is both a popular and important technique with several advantages in plant cultivar identification. However, this technique has not been used widely and efficiently in practical plant identification because the analysis and recording of data generated from fingerprinting and genotyping are tedious and difficult. We developed a novel approach known as a cultivar identification diagram (CID) strategy that uses DNA markers to separate plant individuals in a more efficient, practical, and referable manner. A CID was manually constructed and a polymorphic marker was generated from each polymerase chain reaction for sample separation. In this study, 67 important sea buckthorn cultivars cultivated in China were successfully separated with random amplified polymorphic DNA markers using the CID analysis strategy, with only seven 11-nucleotide primers employed. The utilization of the CID of these 67 sea buckthorn cultivars was verified by identifying 2 randomly chosen groups of cultivars among the 67 cultivars. The main advantages of this 1837 Identification of sea buckthorn cultivars ©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 14 (1): 1836-1845 (2015) identification strategy include fewer primers used and separation of all cultivars using the corresponding primers. This sea buckthorn CID was able to separate any sea buckthorn cultivars among the 67 studied, which is useful for sea buckthorn cultivar identification, cultivar-rightprotection, and for the sea buckthorn nursery industry in China.

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Section: Introductionmentioning

confidence: 99%

A new strategy for complete identification of sea buckthorn cultivars by using random amplified polymorphic DNA markers

Yang

Ding

et al. 2015

Genet. Mol. Res.

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“…Although morphological traits are valuable in identifying genotypes, the results are not completely reliable because morphological traits can be affected by environmental factors and cultivation conditions (STRUSS et al, 2001). These markers directly measure variations at the DNA level and are not affected by environmental factors and developmental stages (SARABI et al, 2010;SARKHOSH et al, 2006).…”

Section: Introductionmentioning

confidence: 99%

Assessment of genetic diversity in the Russian olive (Elaeagnus angustifolia) based on ISSR genetic markers

Asadiar¹,

Rahmani²,

Siami³

2013

Rev. Ciênc. Agron.

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-Elaeagnus is a Eurasian tree with 77 species worldwide. In this study, ISSR markers were used to establish the level of genetic relationships and polymorphism across nine genotypes of Elaeagnus angustifolia collected from 9 different regions of West Azarbaijan province. The ISSR analysis with 11 anchored primers also generated 116 scorable loci, of which 92 were polymorphic (79.3%). The estimated Jaccard similarity coefficient ranged from 0.44 to 0.76 for the ISSR markers. Cluster analysis was carried out, based on the Unweighted Pair Group Method with Arithmetic Averages (UPGMA) and the dendrogram drawn with the help of the NTSYSpc 2.02 software. The analysis revealed 5 main clusters for the ISSR data. According to our results, there is a relatively high genetic distance across E. angustifolia genotypes in the West Azarbaijan province of Iran. Furthermore, it could be inferred that ISSR markers are suitable tools for the evaluation of genetic diversity and relationships within the Elaeagnus genus.

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“…RAPD markers have been widely used in the analysis of genetic relationships and genetic diversity in a number of plant taxa because of its simplicity, speed and relatively low cost compared to other DNA-based markers (Esselman et al 2000;Rout et al 2003;Sheng et al 2006;Yuzbasioglu et al 2006). Nevertheless, dominant inheritance and repeatability of the bands have been the two main limitations with the use of RAPD technique in the assessment of genetic diversity and genetic relationships (Fischer, 2000).…”

Section: Resultsmentioning

confidence: 99%

“…RAPD is a useful DNA-based method for assessment of genetic variation in species and genetic relationships between species due to its simplicity, speed and relatively low cost (Williams et al 1990; Fischer et al 2000). Due to advantages associated with RAPD, it has been widely used in plants to investigate genetic relationships among species and genetic diversity within species (Esselman et al 2000;Rout et al 2003;Castiglioni and De Campos, 2005;Choudhury et al 2006;Fernandez et al 2006;Sheng et al 2006;Yuzbasioglu et al 2006). Some studies have been conducted to elucidate relationships among some Phlomis species by using morphological, anatomical, palynological and cytological traits (Huber-Morath, 1982;Hedge, 1986;Taylor, 1998).…”

Section: *Corresponding Authormentioning

confidence: 99%

Phylogenetic relationships among species of the subsection Dendrophlomis Bentham

Yüzbasioglu¹,

Dadandi²

2008

Electron. J. Biotechnol.

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Abbreviations: AMOVA: analysis of molecular variance ITS: internal transcribed spacer PCR: polymerase chain reaction RAPDs: randomly amplified polymorphic DNA UPGMA: unweighted pair-group method with arithmetic averages This study used randomly amplified polymorphic DNA markers to determine genetic relationships among species of the subsection Dendrophlomis. Twenty accessions of the eleven Phlomis taxa were evaluated to determine genetic variability using fourteen ten mer primers selected from a 125 random oligonucleotide set. These 14 selected primers generated 85 RAPD bands that ranged in size from 200 to 1200 base pairs. Of the total bands, 88% (75) were polymorphic among the samples. Genetic distances among accessions were computed to produce a dendrogram based on UPGMA. Genetic distances ranged from 0.133 (between P. amanica and P. monocephala) to 0.494 (between P. chimerae and P. lunariifolia). The UPGMA tree based on distances has two major groups. The first comprised 9 taxa that were clustered into two subgroups. The first subgroup consisted of P. viscosa, P. lycia, P. amanica and P. monocephala while the second comprised P. lunariifolia, P. bourgaei, P. longifolia var. longifolia, P. grandiflora var. grandiflora and P. grandiflora var. fimbrilligera. The second group comprised 2 species, P. leucophracta and P. chimerae. Species-specific bands were observed for P. lycia, P. leucophracta, P. lunariifolia, P. bourgaei, P. chimerae and P. longifolia var longifolia. *Corresponding authorThe genus Phlomis L. comprises over 100 species including herbs, shrubs and sub-shrubs of the family Lamiaceae (Albaladejo et al. 2005). The genus is divided into two main sections, Phlomis and Phlomoides (Moench, 1794). Both sections are spread from the Mediterranean region to central Asia and China; but while species of the section Phlomoides occur mostly in central Asia and China, species of the section Phlomis appear mainly in the Mediterranean region. Turkey and Iran were indicated as the main centers of diversification in the Mediterranean region for the section Phlomis (Hedge, 1986). In particular, southern and eastern parts of the former and north-western part of the latter were proposed as centers of origin of that section. Nevertheless, Turkey has twice the number of species (34) and also nearly twice a higher endemism rate (57%) of species belonging to section Phlomis compared to Iran, where the numbers are 18% and 33% respectively (Hedge, 1986).Measurement of genetic variation within and between plant species is important for several reasons including delimitation of species, conservation of endangered species and construction of phylogenetic relationships among species. Several kinds of methods were used to measure levels and patterns of genetic variation, which range from Yüzbaşıoğlu, E. and Dadandı, M.Y.2 morphological characterization to various DNA-based markers such as restriction fragment length polymorphism (RFLPs), randomly amplified polymorphic DNA (RAPDs), amplified fragment length polymorphism (AFLPs) ...

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Analysis of the genetic diversity and relationships among and within species of Hippophae (Elaeagnaceae) based on RAPD markers

Cited by 36 publications

References 18 publications

A new strategy for complete identification of sea buckthorn cultivars by using random amplified polymorphic DNA markers

A new strategy for complete identification of sea buckthorn cultivars by using random amplified polymorphic DNA markers

Assessment of genetic diversity in the Russian olive (Elaeagnus angustifolia) based on ISSR genetic markers

Phylogenetic relationships among species of the subsection Dendrophlomis Bentham

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