Analysis of the genetic integrity of rice (Oryza sativa L.) and bean (Phaseolus vulgaris L.) accessions stored in gene banks

Identification and analysis of the genetic integrity of different types of rice resources through SSR markers

“…Molecular markers can directly detect differences and changes in DNA without being restricted by environmental conditions 17 . In recent years, molecular marker detection technology has become a powerful supplement to morphological, cellular and biochemical markers in the identification of genetic integrity and is widely used in testing the genetic integrity of wheat, corn, cotton, rye and other crops 18 , 19 . Since there are few studies on the identification of the genetic integrity of different rice accessions, the method of using molecular technology for detecting genetic integrity is still unclear.…”

Research Article Evaluation of DNA integrity as a potential marker to detect aging in soybean (<i>Glycine</i> <i>max</i>) seeds

“…Cytogenetics, quantitative polymerase chain reaction (qPCR) and random amplified polymorphic DNA (RAPD) meet the above requirement. Cytogenetics is a low-cost method, which has been used for the characterization of aged seed lots of Araucaria angustifolia (Fontes et al, 2001), P. sativum (Khan et al, 2003), Triticum aestivum (Akhter et al, 1992;Menezes et al, 2014), Hordeum vulgare (Akhter et al, 1992) and Oryza sativa and Phaseolus vulgaris (Dantas et al, 2020). qPCR has been shown to be a useful tool in the quantification and evaluation of DNA degradation in soybean (Glycine max) based food products using the cycle threshold (C t ) variation detected using two sets of anchored primers that yielded four different length fragments of two soybean genes (Murray et al, 2009).…”