Analysis of the genetic relationship of gamma-irradiated in vitro mutants derived from standard-type chrysanthemum cv. Migok

Kang, Eunjeong; Lee, Yu‐Mi; Sung, Sang Yeop; Ha, Bo‐Keun; Kim, Sang Hoon; Kim, Dong Sub; Kim, Jin-Baek; Kang, Si-Yong

doi:10.1007/s13580-013-0124-9

Cited by 22 publications

(19 citation statements)

References 17 publications

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“…A total of 18 bands were generated from the five ISSR primers, and eight of them were polymorphic bands representing 44.4% of the total generated bands with an average of 1.6 polymorphic bands per primer pair. These results were similar to that reported for Chrysanthemum (Kang et al, 2013). S1, available online), while the UBC 809 and UBC 842 primers gave the lowest percentage of polymorphic bands (33.3%).…”

Section: Polymorphism Detected By Issr Markersupporting

confidence: 90%

Genetic diversity and variation analysis of mutant lines derived from γ-ray and chemical mutagen treatments in blackberry (Rubus fruticosus)

Ryu

Kim

et al. 2014

Plant Genet. Resour.

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Blackberry is a fruiting berry species with very high nutrient contents. With the recent increasing consumer demand for blackberries, new sources of germplasm and breeding techniques are required to improve blackberry production. This study was carried out to evaluate the genetic diversity (GD) and relationship among 55 blackberry (Rubus fruticosus) mutants derived from g-ray treatment (52 lines) and N-methyl-N 0 -nitrosourea (MNU) treatment (three lines) using an inter-simple sequence repeat marker. A total of 18 bands were amplified with an average of 3.6 bands per primer. Among them, eight bands were identified to be polymorphic with a rate of 44.4%. In addition, the GD information content values were highest in the 60 Gy treatment population and the GD values were higher in the g-ray treatment populations than in the MNU treatment population. According to a cluster analysis, all the mutant lines can be classified into five categories, and the genetic distance was greatest between the 80 Gy-irradiated population and other populations. These results indicate that mutant lines have high GD and can be effectively utilized for improving blackberry breeding.

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Section: Polymorphism Detected By Issr Markersupporting

confidence: 90%

Genetic diversity and variation analysis of mutant lines derived from γ-ray and chemical mutagen treatments in blackberry (Rubus fruticosus)

Ryu

Kim

et al. 2014

Plant Genet. Resour.

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“…The Lady Group cultivars, derived from the original cultivar by radiomutation, could be distinguished from each other by using RAPD markers of only a single primer or sets of two or three primers. Kang et al (2013) compared the polymorphisms, genetic diversity, and genetic distances between the original chrysanthemum cultivar and gamma-irradiated in vitro plants using AFLP markers and identified 83% (866 bands) of the polymorphisms using 12 primer combinations.…”

Section: Discussionmentioning

confidence: 99%

Assessing the Genetic Diversity of Chrysanthemum Cultivars with Microsatellites

Zhang

Wang²,

Ma³

et al. 2013

J. Amer. Soc. Hort. Sci.

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The morphological characteristics of chrysanthemum (Chrysanthemum ×morifolium) are rich in variation. However, as a result of the aneuploid polyploidy of the chrysanthemum genome and the lack of proper tools, the genomic information of this crop is limited. Development of microsatellite markers has been an increasing trend in crop genetic studies because of the applicability of these markers in breeding programs. In this study, we reported the development of a simple sequence repeat in chrysanthemums using a magnetic beads enrichment method. An enriched genomic library with AC and GT microsatellite motifs was constructed, and 53 positive clones were detected by a colony polymerase chain reaction (PCR) technique. Of these clones, 35 showed high-quality sequences, and 35 primer pairs were designed accordingly. Twenty-six (74.29%) of the 35 primer pairs revealed polymorphisms on a set of 40 chrysanthemum cultivars. There were 172 alleles amplified over 26 loci with an average of 6.615 alleles per locus. The mean values of gene diversity corrected for the sample size and the inbreeding coefficient were 0.609 and 0.119 over 26 loci, respectively, which indicated that the majority of the microsatellite loci is highly informative. Cluster analysis based on 26 polymorphic loci demonstrated that the selected cultivars were clustered according to geographical origin. This study shows the isolation efficiency of the magnetic beads technique; the abundance of microsatellites in chrysanthemum; and the potential application for the cultivar classification, the studies on genetic diversity, and molecular breeding of chrysanthemums, which is beneficial to promoting the conservation and sustainable use of this crop.

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“…However, the groups did not appear to be formed based on the ɤ-ray doses. It has been shown that physical mutageneses, such as ion-beam, x-ray, ɤ-ray, sometimes cause random variance (Kurowska et al, 2012;Kang et al, 2013). In contrast to the TE-TRAP, the phylogenetic tree and PCA based on the TD or AFLP systems did not result in any suitable groupings.…”

Section: Discussionmentioning

confidence: 96%

Development of a transposon-based marker system for mutation breeding in sorghum (Sorghum bicolor L.)

Kwon

Ryu

et al. 2016

Genet. Mol. Res.

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ABSTRACT. Under certain circumstances, transposable elements (TE) can create or reverse mutations and alter the genome size of a cell. Sorghum (Sorghum bicolor L.) is promising for plant transposon tagging due to its small genome size and its low content of repetitive DNA. We developed a marker system based on targeted region amplification polymorphisms (TE-TRAP) that uses the terminal inverted repeats (TIRs) of transposons. A total of 3816 class 2 transposons belonging to the PIF/Harbinger family were identified from the whole sorghum genome that produced five primers, including eight types of TIRs. To define the applicability and utilization of TE-TRAP, we used 21 individuals that had been bred after ɤ-ray irradiation. In total, 31 TE- TRAP, 16 TD, and 21 AFLP primer combinations generated 1133, 223, and 555 amplicons, respectively. The percent polymorphic marker was 62.8, 51.1, and 59.3% for the TE-TRAP, TD, and AFLP markers, respectively. Phylogenetic and principal component analyses revealed that TE-TRAP divided the 21 individuals into three groups. Analysis of molecular variance suggested that TE-TRAP had a higher level of genetic diversity than the other two marker systems. After verifying the efficiency of TE-TRAP, 189 sorghum individuals were used to investigate the associations between the markers and the ɤ-ray doses. Two significant associations were found among the polymorphic markers. This TE-based method provides a useful marker resource for mutation breeding research.

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Analysis of the genetic relationship of gamma-irradiated in vitro mutants derived from standard-type chrysanthemum cv. Migok

Cited by 22 publications

References 17 publications

Genetic diversity and variation analysis of mutant lines derived from γ-ray and chemical mutagen treatments in blackberry (Rubus fruticosus)

Genetic diversity and variation analysis of mutant lines derived from γ-ray and chemical mutagen treatments in blackberry (Rubus fruticosus)

Assessing the Genetic Diversity of Chrysanthemum Cultivars with Microsatellites

Development of a transposon-based marker system for mutation breeding in sorghum (Sorghum bicolor L.)

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