Analysis of the Genetic Stability of Banana Tissue Culture Propagated Plantlets cv. Ney Poovan (AB) Using Morphological and Molecular Markers

Bhalang, D.; Prabhuling, G.; Hipparagi, Kulapati; Raghavendra, S.; Prakash, Dhan; Babu, A. Giridhar

doi:10.20546/ijcmas.2018.701.121

Cited by 10 publications

(6 citation statements)

References 15 publications

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“…Differences in regeneration abilities under different treatments may be due to differences in endogenous levels of growth hormones in these tissues. Several researchers have well documented the utility of molecular analysis of in vitro regenerated plantlets [35,45,46]. Somaclonal variation is often induced by the culture media and subculture cycles [47].…”

Section: Discussionmentioning

confidence: 99%

In vitro propagation and assessment of genetic stability in date palm as affected by chitosan and thidiazuron combinations

Al-Mayahi

2022

Journal of Genetic Engineering and Biotechnology

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Background Mass propagation of date palm has attracted the interest of commercial producers. However, this technique still faces many obstacles that hinder production. This study investigated the effect of chitosan (CHT) at various concentrations for the possibility to apply it in combination with thidiazuron (TDZ) on the growth and development of tissue cultures of Barhee cultivar. Results The results showed that CHT and TDZ on in vitro proliferation of Barhee date palm cultivar were significant. The highest response rate and the number of shoots per jar were found in MS media supplemented with 15 mgL–1 CHT and 0.5 mgL–1 TDZ combination. Furthermore, we found that the combined application between 20 mg L−1 CHT+ 1.0 mg L−1 TDZ resulted in the highest shoots content of endogenous IAA, compared with other treatments. At the same time, the data revealed that the maximum cytokinins (CKs) content of shoots occurred in a medium supplemented with 15 mg L−1 CHT and 0.5 mg L−1 TDZ. The genetic stability of the discussed micropropagation protocol was confirmed in this study by DNA-based technique RAPD (random amplified polymorphic DNA). The results may indicate that the micropropagation protocol developed in this research paper was appropriate and applicable for producing genetically stable date palm cv Barhee plants. Conclusion Applying the strategy of culture treatment with (CHT) and (TDZ) can be valuable for improving the propagation of date palm cv Barhee in vitro.

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Section: Discussionmentioning

confidence: 99%

In vitro propagation and assessment of genetic stability in date palm as affected by chitosan and thidiazuron combinations

Al-Mayahi

2022

Journal of Genetic Engineering and Biotechnology

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“…High BA concentrations (15-30 mgL -1 ) could cause somaclonal concentrations in bananas and rice by increasing the number of chromosomes and stimulating morphogenesis by destroying the cell cycle (Bairu et al 2011). BA concentration of 5-8 mgL -1 causes small amounts of somaclonal variations in bananas (<6%) (Bhalang et al 2018), and 3 mgL -1 BA in media could cause somaclonal variations of pineapple plants cultured in vitro compared with parental plants (Kohpaii et al 2017). In this study, genetic diversity in plants cultured in vitro could be compared with parent plants because of the media that included BA, a growth regulator, at a high concentration of 2 ppm.…”

Section: Genetic Similarity Analysismentioning

confidence: 99%

Short Communication: Genetic similarity analysis of in vitro cultivated pineapple (Ananas comosus) from Sipahutar, North Sumatra, Indonesia using ISSR markers

Harahap

NUSYIRWAN²,

AFIVA³

et al. 2022

Biodiversitas

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Abstract. Harahap F, Nusyirwan N, Afiva A, Prasetya E, Suriani C, Hasibuan RFM, Poerwanto R. 2022. Short Communication: Genetic similarity analysis of in vitro cultivated pineapple (Ananas comosus) from Sipahutar, North Sumatra, Indonesia using ISSR markers. Biodiversitas 23: 5623-5628. Sipahutar pineapple from North Sumatra, Indonesia, is considered difficult to breed, resulting in a lack of seed availability. However, tissue culture allows the emergence of somaclonal variations, especially in plantlets undergoing several subculture stages. This study aimed to identify somaclonal variations in Sipahutar pineapple seedlings generated from in vitro culture using Inter Simple Sequence Repeat (ISSR) markers. Fifteen accessions were used in this study consisting of Sipahutar pineapple grown in its original environment, a laboratory environment, acclimatized, and in vitro cultured. Total of 10 primers, of which seven primers detected polymorphisms among the population. This study resulted in a low genetic similarity among the Sipahutar pineapple population. The genetic diversity of Sipahutar pineapple plants propagated by in vitro culture had a similarity level of 75%-94%, showing somaclonal variations in vitro culture. The genetic similarity of pineapple accessions was divided into two main clusters, namely cluster A consisted of 11 accessions and cluster B consisted of 4 accessions. Cluster separation does not correlate to the source of plant materials studied.

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“…Perlakuan BA pada konsentrasi tinggi (15-30 mgL -1 ) menyebabkan variasi somaklonal pada pisang dan padi dengan cara meningkatkan jumlah kromosom (Bairu et al, 2011). Namun demikian, studi lain juga melaporkan bahwa penggunaan BA tidak menyebabkan munculnya variasi somaklonal, atau menyebabkan variasi somaklonal dalam nilai yang kecil (< 6%; Bhalang et al, 2018). Hingga saat ini, pengaruh hormon BA terhadap variasi somaklonal masih diperdebatkan.…”

Section: Bunclassified

PENGUJIAN PENANDA RANDOM AMPLIFIED POLYMORPHISM DNA UNTUK MENGETAHUI KESTABILAN GENETIK KLON JATI (Tectona grandis)

Nurtjahjaningsih¹,

Herawan²,

Rachma

et al. 2018

JPTH

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This study aimed to test RAPD markers to assess genetic stability of teak clones. Two experimental steps were carried out. First, nine RAPD markers were screened to verify the level of polymorphic loci using non clone samples; second, the polymorphic loci were applied to test genetic stability of clones from tissue culture. To test polymorphism levels of the primers, DNA was isolated from eight leaf samples that were collected from a seed orchard located at Watusipat, Gunung Kidul. To verify genetic stability of clones, DNA was isolated from leaf samples of 24 ramets of 3 clones after second sub-culturing. Results showed that amplification of 5 out of 9 RAPD primers were consistent and produced 12 polymorphic loci. The number of polymorphic alleles per locus ranged between 1 and 3; the allele sizes were between 400 and 1050 base pairs (bps). The percentage of polymorphic loci was 100%; it meant that overall loci have high polymorphism level. Based on these loci showed that the 24 ramets are clones; there was no somaclonal variation or high genetic stability. However, these loci need to be validated using more stable DNA markers. ABSTRAKPenelitian ini bertujuan untuk menguji penanda RAPD pada kestabilan genetik klon jati. Pengujian ini melalui dua tahap penelitian yaitu memilih penanda RAPD yang mengamplifikasikan lokus polimorfik pada sampel bukan klon, kemudian target lokus polimorfik tersebut diujikan pada sampel klon hasil kultur jaringan. Sampel DNA bukan klon berasal dari kebun benih jati di Watusipat, Gunung Kidul. Sampel DNA klon berasal dari 24 ramet, dari 3 klon setelah 2 kali sub kultur. Hasil penelitian menunjukkan bahwa 5 dari 9 penanda RAPD pada sampel bukan klon, bersifat konsisten dan menghasilkan 12 lokus polimorfik. Jumlah alel polimorfik per lokus berkisar antara 1 sampai dengan 3; ukuran alel berkisar antara 400 sampai 1050 pasangan basa (bp). Persentase lokus polimorfik bernilai 100% menunjukkan bahwa seluruh lokus mempunyai nilai polymorphism tinggi. Berdasarkan 12 lokus tersebut, 24 ramet menunjukkan genotipe yang sama pada masing-masing klonnya. Namun demikian, target lokus tersebut masih harus divalidasi menggunakan penanda DNA yang sifatnya lebih stabil. Kata kunci: kultur jaringan, pemilihan primer, lokus polimorfik, variasi somaklonal

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Analysis of the Genetic Stability of Banana Tissue Culture Propagated Plantlets cv. Ney Poovan (AB) Using Morphological and Molecular Markers

Cited by 10 publications

References 15 publications

In vitro propagation and assessment of genetic stability in date palm as affected by chitosan and thidiazuron combinations

In vitro propagation and assessment of genetic stability in date palm as affected by chitosan and thidiazuron combinations

Short Communication: Genetic similarity analysis of in vitro cultivated pineapple (Ananas comosus) from Sipahutar, North Sumatra, Indonesia using ISSR markers

PENGUJIAN PENANDA RANDOM AMPLIFIED POLYMORPHISM DNA UNTUK MENGETAHUI KESTABILAN GENETIK KLON JATI (Tectona grandis)

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