Analysis of the Genome and Chromium Metabolism-Related Genes of Serratia sp. S2

Dong, Lanlan; Zhou, Simin; He, Yuan; Jia, Yan; Bai, Qunhua; Deng, Peng; Gao, Jieying; Li, Yingli; Xiao, Hong

doi:10.1007/s12010-017-2639-5

Cited by 20 publications

(4 citation statements)

References 42 publications

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“…Eslami et al [47] previously reported a 22 Kda AzoR from a halophilic bacterium whereas a 29 KDa AzoR was isolated by Nisar et al [48] from a Staphylococcus sp. Dong et al [49] reported many copies of NAD(P)H-dependent AzoR in Serratia sp. S2.…”

Section: Azor Characterizationmentioning

confidence: 99%

Degradation of sulphonated mono and di-azo dye as the sole carbon source inSerratia marcescens: Insights from combined wet and dry lab analysis

Basharat¹,

Yasmin

2023

Preprint

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The high production volume of azo dyes for manufacturing and treating various consumer products leads to deleterious environmental consequences. Bacterial agents present in the environment can degrade these dyes. We, hereby, report the isolation, decolorization and degradation of a mono (Methyl orange) and di-azoic (Congo red) compound of this class of dyes by a versatile bacterium Serratia marcescens. Our isolate showed the capability of sulphonated azo dye utilization/degradation i.e. Methyl orange and Congo red usage, with no inhibitory effects on its growth in the minimal medium. The calorimetric analysis showed 80.83% decolourization of Methyl orange and 92.7% decolourization of Congo red after 7 days of incubation in a shaking incubator at pH: 7 and temperature: 37 degrees Celsius. An azoreductase enzyme of ~25 KDa was detected after SDS-PAGE analysis. Quantitative and qualitative testing of the degradation phenomenon was followed by in silico analysis. Structural modeling followed by molecular docking in Molecular Operating Environment revealed numerous residues involved in binding and assisting degradation. Changes in the apo, holo, and dye-bound enzyme energy profiles were also observed. This is the first study reporting the capability of Serratia marcescens to use azo dyes/sulphonated azo dyes as the sole carbon source and the detailed computational analysis of the degradation phenomenon. We hope that these findings will be of use to environmental scientists, aid in better dye-degrading mutant creation to help craft future remediation strategies for sulphonated azo dyes.

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Section: Azor Characterizationmentioning

confidence: 99%

Degradation of sulphonated mono and di-azo dye as the sole carbon source inSerratia marcescens: Insights from combined wet and dry lab analysis

Basharat¹,

Yasmin

2023

Preprint

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“…In recent years, researchers have discovered some bacteria that can remove Cr(VI), including Serratia sp. S2 [3], Bacillus cereus [4], Bacillus licheniformis [5], and Sporosarcina saromensis [6]. Although most studies on Cr(VI) removal by bacteria have focused on strain isolation and cultivation, mechanistic studies on chromium metabolism genes and proteins in bacteria are also being conducted.…”

Section: Introductionmentioning

confidence: 99%

“…S2. Genome sequencing analysis revealed that its genome contains ChrA, ChrB, and ChrT genes and ChrAB gene clusters related to Cr(VI) metabolism [3]. However, these studies lacked comprehensiveness and systematicity, so we wondered if we could use modern omics techniques to combine genomics and proteomics for a multiomics analysis, forming a "DNA-protein" association network to better understand the molecular response mechanism of bacteria under exogenous Cr(VI) stress.…”

Section: Introductionmentioning

confidence: 99%

Analysis of the Genome of Chromium-resistant Serratia sp. CM01 and Chromium Metabolism Characteristics of the ChrA1-Srpc Gene

Wang,

Li,

Yin

et al. 2023

Preprint

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Objective: Serratia sp. CM01 is a naturally domesticated strain with chromium (Cr) resistance and Cr(VI) reduction ability isolated from a Cr-enriched environment. The genome of Serratia sp. CM01 was sequenced and analysed to identify key genes involved in chromium metabolism. Corresponding engineered bacteria associated with special metabolic features were constructed to further study the function of these genes and their encoded proteins in chromium metabolism. Methods: The whole genome of Serratia sp. CM01 was sequenced, and genes involved in chromium metabolism were analysed. The ChrA1 and Srpc genes were selected and introduced into E. coli using the prokaryotic expression vector pET-28a. The target proteins were identified using sodium dodecyl sulphate‒polyacrylamide gel electrophoresis (SDS‒PAGE) and Western blotting. Chromium tolerance and removal tests were used to assess the chromium metabolic capabilities of the engineered bacteria. Results: The results of genome sequencing revealed that the genome size of Serratia sp. was 4,902,254 bp, from which 12 genes involved in chromium metabolism were screened. The ChrA1 and Srpc genes were chosen, and three engineered bacteria (eChrA1, eSrpc, and eCS) were successfully constructed. The growth of the three engineered bacteria showed no difference under Cr(VI)-free circumstances (P > 0.05). In the presence of Cr(VI), the viable bacterial cell mount during the stabilization phase was eCS > eChrA1 > eSrpc, and the tolerance and removal rate of Cr(VI) was ranked eCS > eChrA1 > eSrpc. Conclusion: The genome of Serratia sp. CM01 contains genes related to chromium metabolism, such as NemA, ChrA1, Srpc and FieF. The ChrA1 and Srpc gene-encoded proteins confer Cr(VI) resistance to engineered bacteria, but the specific mechanism of chromium resistance remains to be further studied. eChrA1 has a stronger anti-Cr(VI) ability than eSrpc. The eCS-engineered bacterial strain with ChrA1 and Srpcgenes was more resistant to hexavalent chromium, and it has the potential to handle Cr(VI) pollution in the virtual environment.

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“…Chromium pollutants can be mutagenic, genotoxic, and carcinogenic in humans (Liu et al, 2015;Huang et al, 2017). Cr 6+ compounds can be easily absorbed by different parts of the human body, such as the skin, digestive tract, and respiratory tract (Dong et al, 2018). In addition, chromium can induce cell DNA damage and gene expression (Alimba et al, 2016;Wang et al, 2016;Karthik et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

Chromium bioremediation of batik industrial wastewater using a consortium of sulfate-reducing bacteria from forested wetland soil

Syawaluddin

Retnaningrum

2022

J.Degrade.Min.Land Manage.

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Chromium pollutants in textile wastewater can be removed by bioremediation using sulfate-reducing bacteria (SRB) from forested wetland soil. Biostimulation of carbon sources in the form of molasses and a supporting material in the form of zeolite to trap bacteria and create biofilms can improve the ability of SRB to bioremediate chromium. The batch bioremediation technique was further examined by including molasses, a combination of molasses and zeolite, and SRB, which has been adapted to acclimatize wastewater that is diluted two times. Adaptive SRB aged 7 days, which had reached the exponential growth phase, showed optimal bioremediation activity when molasses and zeolite were added. Results of further observations of the consortium on continuous bioremediation with the same treatment showed decontamination of chromium efficiency that reached about 94%. In addition, pH values decreased efficiency at approximately 7.3 in 14 days of incubation. The biological oxygen demand, chemical oxygen demand, and sulfate concentrations also decreased at around 89%, 92%, and 91%, respectively. SRB immobilization with zeolite-induced biofilm formation was observed at 9 days, and it further increased at 14 days. SRB cells observed were attached to the surface of the zeolite, between cells connected to each other by extracellular polymeric substances. The mass of sulfur and chromium on the surface of the zeolite increased from the 9th and 14th days. Sulfur increased from 0.07% to 0.27%, whereas chromium increased from 0.21% to 0.84%. The increase in the percentage of the two elements on the zeolite surface indicated the decontamination of sulfate and chromium pollutants in wastewater.

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Analysis of the Genome and Chromium Metabolism-Related Genes of Serratia sp. S2

Cited by 20 publications

References 42 publications

Degradation of sulphonated mono and di-azo dye as the sole carbon source inSerratia marcescens: Insights from combined wet and dry lab analysis

Degradation of sulphonated mono and di-azo dye as the sole carbon source inSerratia marcescens: Insights from combined wet and dry lab analysis

Analysis of the Genome of Chromium-resistant Serratia sp. CM01 and Chromium Metabolism Characteristics of the ChrA1-Srpc Gene

Chromium bioremediation of batik industrial wastewater using a consortium of sulfate-reducing bacteria from forested wetland soil

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