Analysis of the Genome Structure of the Nonpathogenic Probiotic
            <i>Escherichia coli</i>
            Strain Nissle 1917

Grozdanov, Lubomir; Raasch, Carsten; Schulze, Jürgen P.; Sonnenborn, U.; Gottschalk, Gerhard; Hacker, Jörg; Dobrindt, Ulrich

doi:10.1128/jb.186.16.5432-5441.2004

Cited by 323 publications

(302 citation statements)

References 60 publications

Supporting

Mentioning

290

Contrasting

Unclassified

Order By: Relevance

“…No clear distinction can be drawn between ExPEC and commensal E. coli (55). ExPEC can stably colonize the host intestine and are predominant in Ϸ20% of healthy people (54).…”

Section: Small Gene Clusters͞single Genes That May Contribute To Urovmentioning

confidence: 99%

How to become a uropathogen: Comparative genomic analysis of extraintestinal pathogenic Escherichia coli strains

Brzuszkiewicz

Brüggemann

Liesegang

et al. 2006

Proc. Natl. Acad. Sci. U.S.A.

Self Cite

330

275

View full text Add to dashboard Cite

Uropathogenic Escherichia coli (UPEC) strain 536 (O6:K15:H31) is one of the model organisms of extraintestinal pathogenic E. coli (ExPEC). To analyze this strain's genetic basis of urovirulence, we sequenced the entire genome and compared the data with the genome sequence of UPEC strain CFT073 (O6:K2:H1) and to the available genomes of nonpathogenic E. coli strain MG1655 (K-12) and enterohemorrhagic E. coli. The genome of strain 536 is Ϸ292 kb smaller than that of strain CFT073. Genomic differences between both UPEC are mainly restricted to large pathogenicity islands, parts of which are unique to strain 536 or CFT073. Genome comparison underlines that repeated insertions and deletions in certain parts of the genome contribute to genome evolution. Furthermore, 427 and 432 genes are only present in strain 536 or in both UPEC, respectively. The majority of the latter genes is encoded within smaller horizontally acquired DNA regions scattered all over the genome. Several of these genes are involved in increasing the pathogens' fitness and adaptability. Analysis of virulence-associated traits expressed in the two UPEC O6 strains, together with genome comparison, demonstrate the marked genetic and phenotypic variability among UPEC. The ability to accumulate and express a variety of virulence-associated genes distinguishes ExPEC from many commensals and forms the basis for the individual virulence potential of ExPEC. Accordingly, instead of a common virulence mechanism, different ways exist among ExPEC to cause disease.fitness ͉ genome comparison ͉ uropathogenic Escherichia coli

show abstract

“…No clear distinction can be drawn between ExPEC and commensal E. coli (55). ExPEC can stably colonize the host intestine and are predominant in Ϸ20% of healthy people (54).…”

Section: Small Gene Clusters͞single Genes That May Contribute To Urovmentioning

confidence: 99%

How to become a uropathogen: Comparative genomic analysis of extraintestinal pathogenic Escherichia coli strains

Brzuszkiewicz

Brüggemann

Liesegang

et al. 2006

Proc. Natl. Acad. Sci. U.S.A.

Self Cite

330

275

View full text Add to dashboard Cite

show abstract

“…Nissle 1917 is an excellent colonizer in mice and humans and has been detected in infants up to 6 months after initial administration (17)(18)(19)(20)(21). Analysis of the genome structure of Nissle 1917 reveals the presence of numerous fitness factors, including multiple iron uptake systems, proteases, bacteriocins, fimbriae, and other adhesions (22). This strain does not produce any known virulence factors and is known to be safe for humans because it has been widely used as a probiotic treatment for intestinal disorders, such as diarrhea, irritable bowel disease, ulcerative colitis, and Crohn's disease (23)(24)(25)(26).…”

Section: Resultsmentioning

confidence: 99%

Toward a live microbial microbicide for HIV: Commensal bacteria secreting an HIV fusion inhibitor peptide

Rao

McHugh

et al. 2005

Proc. Natl. Acad. Sci. U.S.A.

127

View full text Add to dashboard Cite

“…The sudden drop in person C, detected at day 62, could not be explained. At the end of the experiment, around day 190, the total E. coli counts varied from 1.1·10 5 to 8.8·10 6 CFU/g (geometric mean 7.5·10 5 CFU/g). It was investigated which proportion of the detected E. coli could be attributed to the Symbiofl or2 intake.…”

Section: Colonization Potential Of Symbioflor2 E Coli Following a Simentioning

confidence: 99%

“…An example is Escherichia coli Nissle 1917, which has been used in probiotic applications for decades [5]. When its genome was analyzed, of all E. coli genomes then available, it showed most similarity to that of strain E. coli CFT073, a pathogenic E. coli causing urinary tract infections [6,7]. Nevertheless, its documented history of safe use suggests that the presence of genes that, in a different genomic content, have been shown to contribute to virulence, can be considered as nonproblematic, when found in the context of the Nissle genome.…”

Section: Introductionmentioning

confidence: 99%

Virulence genes in a probioticE. oliproduct with a recorded long history of safe use

Wassenaar¹,

Zschüttig²,

Beimfohr³

et al. 2015

European Journal of Microbiology and Immunology

View full text Add to dashboard Cite

The probiotic product Symbioflor2 (DSM 17252) is a bacterial concentrate of six different Escherichia coli genotypes, whose complete genome sequences are compared here, between each other as well as to other E. coli genomes. The genome sequences of Symbioflor2 E. coli components contained a number of virulence-associated genes. Their presence seems to be in conflict with a recorded history of safe use, and with the observed low frequency of adverse effects over a period of more than 6 years. The genome sequences were used to identify unique sequences for each component, for which strain-specific hybridization probes were designed. A colonization study was conducted whereby five volunteers were exposed to an exceptionally high single dose. The results showed that the probiotic E. coli could be detected for 3 months or longer in their stools, and this was in particular the case for those components containing higher numbers of virulence-associated genes. Adverse effects from this long-term colonization were absent. Thus, the presence of the identified virulence genes does not result in a pathogenic phenotype in the genetic background of these probiotic E. coli.

show abstract

Analysis of the Genome Structure of the Nonpathogenic Probiotic Escherichia coli Strain Nissle 1917

Cited by 323 publications

References 60 publications

How to become a uropathogen: Comparative genomic analysis of extraintestinal pathogenic Escherichia coli strains

How to become a uropathogen: Comparative genomic analysis of extraintestinal pathogenic Escherichia coli strains

Toward a live microbial microbicide for HIV: Commensal bacteria secreting an HIV fusion inhibitor peptide

Virulence genes in a probioticE. oliproduct with a recorded long history of safe use

Contact Info

Product

Resources

About