Analysis of the genotoxic potential of low concentrations of Malathion on the Allium cepa cells and rat hepatoma tissue culture

Bianchi, Jaqueline; Mantovani, Mário Sérgio; Marin-Morales, Maria Aparecida

doi:10.1016/j.jes.2015.03.034

Cited by 52 publications

(35 citation statements)

References 74 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Root meristems of Allium cepa L. (onion) are regarded in the scientific community as an effective bioassay for the assessment of acute toxicity at the cellular level of chemical compounds, since they have low chromosome number (2n = 16), which favors detection of chromosomal or clastogenic changes, alterations in the mitotic spindle or aneugenic activities, and changes in cell proliferation (Türkoğlu, 2007;Neves et al, 2014;Bianchi et al, 2015). This test system is internationally accepted by research agencies as an assessment tool with accurate sensitivity for analysis of cytotoxicity, genotoxicity and mutagenicity of the substance of interest, since the results obtained mostly show satisfactory similarity to those obtained via animal testing systems and cell cultures (Herrero et al, 2012;Lacerda et al, 2014;Tabrez et al, 2011;Gomes et al, 2013;Dliveira et al, 2013;Campos-Ventura et al, 2016;Moura et al, 2016;Santana et al, 2016;Bezerra et al, 2016;Nunes et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

Cytotoxic, genotoxic and mutagenic potential of UHT whole milk

2017

View full text Add to dashboard Cite

This study evaluated the action at the cellular level of long life whole milk, full type of six renowned companies operating in the Brazilian market, as well as in other South American countries. The evaluation was performed using root meristem cells of Allium cepa L., at exposure times 24 and 48 hours, directly in milk products marketed. The results indicated that all the milk samples reduced root meristem cell proliferation, proving, in this study, a significant cytotoxic effect. Still, exposure to milk resulted in a significant frequency of mitotic spindle changes in meristem cells, characterizing these foods as genotoxic and mutagenic under the study conditions. It can be concluded that the long life milk samples caused significant genetic instability to cells of the examined tissue. The results obtained for cytotoxic, mutagenic and genotoxic action of these long life milks are of great relevance because, to date, there are no published toxicity studies on such foods and food additives present in the composition.

show abstract

Section: Introductionmentioning

confidence: 99%

Cytotoxic, genotoxic and mutagenic potential of UHT whole milk

2017

View full text Add to dashboard Cite

show abstract

“…The meristem tissue of roots of Allium cepa L. (onion) is regarded in the scientific community as an effective bioassay for the assessment of acute toxicity at the cellular level of chemical compounds, due to low chromosome number (2n = 16), which facilitates the detection of chromosomal abnormalities and mitotic spindle changes (Neves, Ferreira, Lima, & Peron, 2014, Bianchi, Mantovani, & Marin-Morales, 2015. This test system is internationally accepted by research agencies as an assessment tool with accurate sensitivity for initial analysis of cytotoxicity, genotoxicity and mutagenicity of the substance of interest, as the results obtained show satisfactory similarity to those observed with animal test systems and cell cultures (Türkoğlu, 2007, Herrero et al, 2011, Tabrez et al, 2011, Gomes et al, 2013, Lacerda, Malaquias, & Peron, 2014, CamposVentura & Marin-Morales, 2016, Moura et al, 2016, Campus-Pereira, Gonçalves, Hara, & Marin-Morales, 2016.…”

Section: Introductionmentioning

confidence: 99%

<b>Processed fruit juice ready to drink: screening acute toxicity at the cellular level

Silva

Sales

Santos

et al. 2017

Acta Sci. Biol. Sci.

View full text Add to dashboard Cite

ABSTRACT. The present study evaluated the acute toxicity at the cellular level of processed juice ready for consumption Orange and Grape flavors, produced by five companies with significant influence on the food market of South American countries, especially in Brazil. This evaluation was performed in root meristem cells of Allium cepa L., at the exposure times of 24 and 48 hours, directly with marketed liquid preparations. Based on the results, it was found that fruit juices, of all companies considered, promoted significant antiproliferative effect to root meristems at the exposure time of 24 hours and resulted in at both exposure times, statistically significant number of mitotic spindle changes and chromosomal breaks. Therefore, under the study conditions, all juice samples analyzed were cytotoxic, genotoxic and mutagenic to root meristem cells. These results indicate that such beverages have relevant potential to cause cellular disorders and, thus, need to be evaluated more fully in more complex test systems, as those in rodents, and then establish specific toxicity at the cellular level of these juices and ensure the well-being of those who consume them.Keywords: industrialized juice, cytotoxicity, genotoxicity, mutagenicity, meristem tissue.Sucos de frutas industrializados prontos para consumo: screening da toxicidade aguda em nível celular RESUMO. Objetivou-se neste trabalho avaliar a toxicidade aguda em nível celular de sucos industrializados prontos para beber, sabor laranja e uva, de cinco empresas alimentícias de reconhecida reputação no mercado de alimentos em países da América do Sul, especialmente o Brasil. Esta avaliação se deu por meio das células meristemáticas de raízes de Allium cepa L., nos tempos de exposição 24 e 48h, diretamente nos preparados líquidos comercializados. Com base nos resultados obtidos verificou-se que os sucos de frutas, de todas as empresas consideradas, promoveram expressivo efeito antiproliferativo aos meristemas de raízes já no tempo de exposição 24h, e ocasionaram número estatisticamente significativo de alterações de fuso mitótico e quebras cromossômicas nas células do tecido analisado em todo o tempo de análise. Portanto, nas condições de estudo estabelecidas, os sucos das empresas avaliadas foram citotóxicos, genotóxicos e mutagênicos. Estes resultados são importantes em razão de indicarem que tais alimentos têm relevante potencial em causar distúrbios celulares e, portanto, devem ser avaliados em sistemas com testes mais complexo, como os em roedores, para, dessa forma, estabelecer com propriedade a toxicidade em nível celular desses alimentos e assegurar o bem-estar daqueles que os consomem.Palavras-chave: sucos industrializados, citotoxicidade, genotoxicidade, mutagenicidade, tecido meristemático.

show abstract

“…The root meristem of Allium cepa L. (onion) is considered in the scientific field as an efficient bioassay for the initial screening of genetic toxicity of chemical compounds because of the reduced chromosomal number (2n = 16), which favors the detection of mitotic spindle or aneugenic changes, and disturbances in cellular proliferation index (NEVES et al, 2014;MANTOVANI;. It is a bioassay accepted internationally by research agencies as an instrument for the evaluation with accurate sensitivity to analyze the cytotoxicity and genotoxicity of the substance of interest, since the results obtained demonstrate, in most cases, satisfactory similarity to those obtained through animal testing systems and in cell cultures (TÜKOĞLU, 2007;HERRERO et al, 2011;LACERDA;TABREZ et al, 2011;GOMES et al, 2013;OLIVEIRA et al, 2013;CAMPOS;MARIN-MORALES, 2016;MOURA et al, 2016.…”

Section: Introductionmentioning

confidence: 99%

Industrial milk powder in bioassays for evaluation of cytotoxicity and genotoxicity

Moura¹,

Marques²,

Pereira³

et al. 2017

Biosci. J.

View full text Add to dashboard Cite

Considering the widespread consumption of milk powder by the general population as well as the lack of studies on the toxicity of such industrialized foods, this study evaluated the cytotoxic and genotoxic potential of powdered milk from four reputed companies in the food market of Brazil and other South American countries. Milk samples were evaluated in root meristem cells of Allium cepa L., at concentrations of 0.065 and 0.13 g/mL, for 24 and 48 hours of exposure; and by means of cell viability in culture of cells of normal lineage, via MTT test, for 24 hours, at concentrations of 0.016; 0.032; 0.065 and 0.13g/mL. The concentration 0.13 g/mL was the one suggested for consumption in all milk packages evaluated in this study. In A. cepa, we observed that the milks, at both concentrations and at the two exposure times investigated, reduced the cellular proliferation of root meristems demonstrating a significant cytotoxicity. Furthermore, 0.13g/mL milks at the exposure time of 24h induced an expressive frequency of cellular alterations in the plant tissue, showing to be genotoxic. In the in vitro evaluation, three milks at 0.065 g/mL and all milks at 0.13 g/mL have significantly reduced cell viability, proving to be cytotoxic to the analyzed cell culture. Therefore, under the studied conditions, the powdered milks evaluated caused significant genetic instability to the cells of the test systems used.

show abstract

Analysis of the genotoxic potential of low concentrations of Malathion on the Allium cepa cells and rat hepatoma tissue culture

Cited by 52 publications

References 74 publications

Cytotoxic, genotoxic and mutagenic potential of UHT whole milk

Cytotoxic, genotoxic and mutagenic potential of UHT whole milk

<b>Processed fruit juice ready to drink: screening acute toxicity at the cellular level

Industrial milk powder in bioassays for evaluation of cytotoxicity and genotoxicity

Contact Info

Product

Resources

About