Analysis of the Glyphodes pyloalis larvae immune transcriptome in response to parasitization by its endoparasitoid, Aulacococentrum confusum

Sheng, Sheng; Wang, Jiao; Chu, Jie; Ding, Jian-Hao; Liu, Zhixiang; Jiang, De-Lei; Liang, Xinhao; Shao, Zuo-min; Wang, Jun; Wu, Fu‐An

doi:10.1016/j.cbd.2021.100803

Cited by 6 publications

(7 citation statements)

References 47 publications

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“…The Hsp70 and Hsp90 genes were identified from the previously constructed transcriptome dataset (ACC.SRR9959773, Sheng et al, 2021 ). ORFfinder 1 was used to predict the open reading frames (ORFs) of GpHsps and CLUSTALX analysis was conducted to obtain aligned amino acid sequences.…”

Section: Methodsmentioning

confidence: 99%

Characterization, and Functional Analysis of Hsp70 and Hsp90 Gene Families in Glyphodes pyloalis Walker (Lepidoptera: Pyralidae)

et al. 2021

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Glyphodes pyloalis Walker (Lepidoptera: Pyralidae) is a destructive mulberry pest, causing great damage to mulberry in China. Heat shock proteins (Hsps) are involved in various signal pathways and regulate lots of physiological processes in insects. The function of Hsps in G. pyloalis, however, has still received less attention. Here, we identified five Hsp genes from G. pyloalis transcriptome dataset including two Hsp70 family genes (GpHsp71.3 and GpHsp74.9) and three Hsp90 family genes (GpHsp82.4, GpHsp89, and GpHsp93.4). Quantitative Real-time PCR validation revealed that all Hsps of G. pyloalis have significant expression in pupal and diapause stage, at which the larvae arrest the development. Expressions of GpHsp71.3 and GpHsp82.4 were increased significantly after thermal treatment at 40°C, and this upregulation depended on heat treatment duration. Furthermore, silencing GpHsp82.4 by RNA interference led to a significant increase in mortality of G. pyloalis larvae under the heat stress compared to the control group. After starvation stress, the expression levels of GpHsp82.4 and GpHsp93.4 were significantly increased. At last, after being parasitized by the parasitoid wasp Aulacocentrum confusum, Hsp70 and Hsp90 genes of G. pyloalis were decreased significantly in the early stage of parasitization and this moderation was affected by time post-parasitization. This study highlights the function of G. pyloalis Hsps in response to environmental stress and provides a perspective for the control of this pest.

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Section: Methodsmentioning

confidence: 99%

Characterization, and Functional Analysis of Hsp70 and Hsp90 Gene Families in Glyphodes pyloalis Walker (Lepidoptera: Pyralidae)

et al. 2021

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“…The GpPGRP‐S 2 and GpPGRP‐S 3 sequences were identified from the G. pyloalis transcriptome (Sheng et al, 2021), and 15 detoxification genes ( GpCXE1 , GpCXE2 , GpCXE3 , GpCXE4 , GpCXE5 , GpCXE6 , GpCXE7, GpGSTd , GpGSTe , GpGSTo , GpGSTs , GpGSTu , GpGSTz , GpP450 , and GpP450‐TBP ) were selected randomly from this transcriptome. Quantitative real‐time polymerase chain reaction (qRT‐PCR) was used to validate the expression levels of GpPGRP s in different time and tissues and the expression levels of detoxification genes in G. pyloalis larvae.…”

Section: Methodsmentioning

confidence: 99%

“…The GpPGRP-S2 and GpPGRP-S3 sequences were identified from the G. pyloalis transcriptome (Sheng et al, 2021), and 15 detoxification genes (GpCXE1, GpCXE2, GpCXE3, GpCXE4, GpCXE5, GpCXE6, GpCXE7, GpGSTd, GpGSTe, GpGSTo, GpGSTs, GpGSTu, GpGSTz, GpP450, and GpP450-TBP) were selected randomly from this transcriptome.…”

Section: Expression Pattern Of Gppgrps and Detoxification Genesmentioning

confidence: 99%

A role of peptidoglycan recognition protein in mediating insecticide detoxification in Glyphodes pyloalis

Jiang

Ding

Liu

et al. 2021

Arch Insect Biochem Physiol

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Glyphodes pyloalis Walker has become one of the most significant mulberry pests, and it has caused serious economic losses in major mulberry growing regions in China. Peptidoglycan recognition proteins (PGRPs) are responsible for initiating and regulating immune signalling pathways in insects. However, their roles responding to chemical pesticides is still less known. This study aimed to investigate the possible detoxication function of GpPGRP‐S2 and GpPGRP‐S3 in G. pyloalis in response to chlorfenapyr and phoxim. The chlorfenapyr and phoxim treatment significantly induced the expression level of GpPGRP‐S3 at 48 h. In addition, the expression levels of GpPGRP‐S2 and GpPGRP‐S3 in the chlorfenapyr/phoxim treatment group were significantly higher in midgut than those in the control group at 48 h. The results of the survival experiment showed that silencing either GpPGRP‐S2 or GpPGRP‐S3 would not influence the survival rate of G. pyloalis which treated with phoxim, however, silencing GpPGRP‐S2 or GpPGRP‐S3 would cause G. pyloalis to be more easily killed by chlorfenapyr. The expression of carboxylesterase GpCXE1 was significantly induced by chlorfenapyr/phoxim treatment, while it was suppressed once silenced GpPGRP‐S2 followed with chlorfenapyr treatment or silenced GpPGRP‐S3 followed with phoxim treatment. These results might suggest that under the chlorfenapyr/phoxim treatment condition, the connection between GpPGRPs and detoxification genes in insect was induced to maintain physiological homeostasis; and these results may further enrich the mechanisms of insects challenged by insecticides.

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“…In parallel, a key study analyzing the effect of parasitism on host gene regulation was that of Chevignon et al (2015) [ 20 ], in which transcriptomes were obtained from the unparasitized and parasitized Manduca sexta (Lepidoptera: Sphingidae) and the specific effects of Cotesia congregata (Hymenoptera: Braconidae) parasitism on host gene expression were revealed. Recently, to gain insight into the effects of parasitization by Aulacocentrum confusum (Hymenoptera: Braconidae) on host Glyphodes pyloalis (Lepidoptera: Pyralidae), Sheng et al (2021) [ 21 ] conducted a transcriptome analysis between parasitized and unparasitized host and identified a battery of differently expressed immune-related genes in parasitized larvae. In a follow-up study, RNA-seq was used to quantify the gene expression levels of the host midgut after parasitization, revealing that the metabolic pathway-related and immune genes were significantly upregulated [ 22 ].…”

Section: Introductionmentioning

confidence: 99%

Host Transcriptome Analysis of Spodoptera frugiperda Larvae Parasitized by Microplitis manilae

Gulinuer

Xing

Yang

2023

Insects

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It has been extensively found that parasitoids manipulate host physiology to benefit the survival and development of their offspring. However, the underlying regulatory mechanisms have not received much attention. To reveal the effects of parasitization of the larval solitary endoparasitoid Microplitis manilae (Hymenoptera: Braconidae) on host Spodoptera frugiperda (Lepidoptera: Noctuidae), one of the most destructive agricultural pests in China, deep-sequencing-based transcriptome analysis was conducted to compare the host gene expression levels after 2 h, 24 h, and 48 h parasitization. A total of 1861, 962, and 108 differentially expressed genes (DEGs) were obtained from the S. frugiperda larvae at 2 h, 24 h, and 48 h post-parasitization, respectively, compared with unparasitized controls. The changes in host gene expressions were most likely caused by the injection of wasp parasitic factors, including PDVs, that were injected along with the eggs during oviposition. Based on the functional annotations in GO and KEGG databases, we revealed that most DEGs were implicated in host metabolism and immunity. Further analysis of the common DEGs in three comparisons between the unparasitized and parasitized groups identified four genes, including one unknown and three prophenoloxidase (PPO) genes. Moreover, 46 and 7 common DEGs involved in host metabolism and immunity were identified at two or three time points after parasitization, respectively. Among these, most DEGs showed increased expressions at 2 h post-wasp parasitization while exhibiting significantly decreased expression levels at 24 h post-parasitization, demonstrating the expression regulations of M. manilae parasitization on host metabolism and immune-related genes. Further qPCR verification in 20 randomly selected DEGs confirmed the accuracy and reproducibility of the gene expression profiles generated from RNA-seq. This study reveals the molecular regulatory network about how host insects respond to wasp parasitism, laying a solid foundation for revealing the physiological manipulation of wasp parasitization on host insects, which facilitates the development of biological control practices for parasitoids.

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Analysis of the Glyphodes pyloalis larvae immune transcriptome in response to parasitization by its endoparasitoid, Aulacococentrum confusum

Cited by 6 publications

References 47 publications

Characterization, and Functional Analysis of Hsp70 and Hsp90 Gene Families in Glyphodes pyloalis Walker (Lepidoptera: Pyralidae)

Characterization, and Functional Analysis of Hsp70 and Hsp90 Gene Families in Glyphodes pyloalis Walker (Lepidoptera: Pyralidae)

A role of peptidoglycan recognition protein in mediating insecticide detoxification in Glyphodes pyloalis

Host Transcriptome Analysis of Spodoptera frugiperda Larvae Parasitized by Microplitis manilae

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