Analysis of the human karyotype using a reassociation technique

Comparative Banding Pattern Analyses in Human Chromosomes Induced by Urea and Trypsin Treatments

“…No difference has been observed between the two X-chromosomes in the female. The patterns produced by these two methods are also similar to those shown by quinacrine fluorescence (Caspersson et al 1970(Caspersson et al , 1971) and other techniques by Giemsa staining (Sumner et al 1971;Schnedl 1971;Drets and Shaw 1971). In contrast to the fluorescence technique, dark stained bands can also be detected by the present techniques at the centromere regions of most chromosomes.…”

“…tions followed by Giemsa staining (Sumner et al 1971;Schnedl 1971;Drets and Shaw 1971). These techniques have become available to identify each chromosome pair in the human complement.…”

Differential staining of cultured bone marrow cell chromosomes of Rana pipiens.

“…G-, C-, and Q-banding patterns were reported for T. alpestris apuanus, T. cristatus carnifex, T. marmoratus marmoratus, T. italicus, and T. vulgaris meridionalis ). This latter work involved the production of G-bands according to Drets and Shaw (1971), Dutrillaux (1971), Sumner et al, (1971), andSchnedl (1971). C-bands have also been reported on the lampbrush chromosomes of T. marmoratus (Batistoni et al 1974).…”

“…Slides were either air-dried or flame-dried, depending on the specific banding method studied. The method of Schnedl (1971) yielded optimal results when chromosomes were exposed to 0.07N NaOH for 90 sec, incubated for 24 hr in Sorenson buffer (pH 6.8), and stained in Giemsa for 40min. Since dotted regions correspond across both sister chromatids, there is a suggestion of banding in some of the chromo somes (Fig.…”

A phylokaryotypic evaluation of the genus Tursiops (family Delphinidae)

“…In many species C-band constitutive heterochromatin is primarily composed of highly repetitious satellite DNA (Yunis, et al, 1971;Schedl, 1971;Jones, 1977;Comings, 1978) although a small portion of constitutive heterochromatin does not contain repetitious DNA (Comings and Mattochia, 1972;Arrighi, et al, 1974). Satellite DNAs are DNAs of different buoyant densities than the main bulk of DNA when separated by high speed gradient ultracentrifugation (Kit, 1961).…”