Analysis of the humoral immune response against total and recombinant antigens of Leishmania infantum: correlation with disease progression in canine experimental leishmaniasis

Nieto, C. G.; Garcia-Alonso, M.; Requena, José Marı́a; Mirón, C.; Soto, Manuel; Alonso, Carlos; Navarrete, Ignacio

doi:10.1016/s0165-2427(98)00213-x

Cited by 101 publications

(75 citation statements)

References 22 publications

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“…Interestingly, humoral immune responses against histone H2b were recently reported in dogs with canine VL. Abs to histone H2b as well as other members of the histone family were detected after the dogs developed VL due to a Leishmania infantum infection (35,36). These data suggest that histone H2b is available for immune recognition not only in situations where the immune system has controlled the infection but also in situations where the parasite has evaded protective immune mechanisms.…”

Section: Discussionmentioning

confidence: 83%

Identification and Characterization of T Cell-Stimulating Antigens from Leishmania by CD4 T Cell Expression Cloning

Probst

Stromberg

Ghalib

et al. 2001

The Journal of Immunology

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Persistent immunity against Leishmania infections in humans is mediated predominantly by CD4+ T cells of the Th1 phenotype. Herein we report the expression cloning of eight Leishmania Ags using parasite-specific T cell lines derived from an immune donor. The Ags identified by this technique include the flagellar proteins α- and β-tubulin, histone H2b, ribosomal protein S4, malate dehydrogenase, and elongation factor 2, as well as two novel parasite proteins. None of these proteins have been previously reported as T cell-stimulating Ags from Leishmania. β-tubulin-specific T cell clones generated against Leishmania major amastigotes responded to Leishmania-infected macrophages and dendritic cells. IFN-γ enzyme-linked immunospot analysis demonstrated the presence of T cells specific for several of these Ags in PBMC from self-healing cutaneous leishmaniasis patients infected with either Leishmania tropica or L. major. The responses elicited by Leishmania histone H2b were particularly striking in terms of frequency of histone-specific T cells in PBMC (1 T cell of 6000 PBMC) as well as the percentage of responding donors (86%, 6 of 7). Ags identified by T cells from immune donors might constitute potential vaccine candidates for leishmaniasis.

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Section: Discussionmentioning

confidence: 83%

Identification and Characterization of T Cell-Stimulating Antigens from Leishmania by CD4 T Cell Expression Cloning

Probst

Stromberg

Ghalib

et al. 2001

The Journal of Immunology

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“…Recent investigations disclosed that the IgG1 subtype of immunoglobulin is predominant or increasing in the severe canine kala-azar (Solano-Gallego et al 2001, Santana et al 2002, Mendes et al 2003, Nieto et al 1999. The use of anti-IgG1 conjugates in diagnostic and prognostic serological assays used for canine control (Badaró et al 1997, Borja-Cabrera et al 1999) might give an enhanced impact on the reduction of the incidence of the disease.…”

Section: Discussionmentioning

confidence: 99%

Improving methods for epidemiological control of canine visceral leishmaniasis based on a mathematical model. Impact on the incidence of the canine and human disease

Palatnik-de-Sousa

Batista-de-Melo

Borja-Cabrera

et al. 2004

An. Acad. Bras. Ciênc.

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The mathematical model described by Dye (1996) condemned the epidemiological canine visceral leishmaniasis control campaign, considering it non-efficient. Using this model, we mathematically demonstrate that the control is not efficient, only at low κ values (rate at which latent and infectious dogs are lost by the destruction program) which match the canine seropositivity observed in the field by the immunofluorescency (IF) blood eluates analysis. With higher k values, corresponding to IF (κ = 0.07) or ELISA (κ = 0.25) results in sera samples, the number of infectious dogs declines to a R o = 1 or R o = 0, respectively, interrupting the transmission and the advancement of epidemics. We also experimentally demonstrate that the dog removal, following the results of IF of sera, instead of eluates lead to a 57% (p < 0.005) decrease in canine cases and 87.5% (p < 0.005) in human cases. Our mathematical and experimental results indicate that the control campaign become more efficient by enhancing the sensitivity of the diagnostic assay.

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“…For the initial diagnosis of leishmaniasis, the ELISA procedure described by Nieto et al [27] was applied. Briefly, the antigen used for the determinations was obtained from late-log phase cultures of L. infantum promastigotes (M/CAN/ES/ 88/CHUMI, LEM 2002).…”

Section: Enzyme-linked Immunosorbent Assay (Elisa)mentioning

confidence: 99%

SDS-PAGE and Western blot of urinary proteins in dogs with leishmaniasis

et al. 2003

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-Canine leishmaniasis is an endemic disease in the Mediterranean area caused by the protozoan Leishmania infantum, which usually produces renal failure. Sodium dodecyl sulphate polyacrylamide gel electrophoresis and Western blot using antibodies to IgG and IgA from dogs were carried out in the urine of 22 dogs with leishmaniasis diagnosed by ELISA and confirmed by PCR, and 20 healthy dogs. The results were compared to renal function laboratory tests and to those from a histopathological study of the kidneys from sick animals that died naturally or were euthanized. Five different bands with molecular weights ranging from 10 to 110 kDa were obtained from the electrophoresis of the urine of healthy dogs. 33.5% of total proteins corresponded to low molecular weight proteins and the other proteins had middle and high molecular weights. However, in the group with leishmaniasis, a maximum of 11 different bands with molecular weights ranging from 10 kDa to 150 kDa were displayed in the electrophoresis of the urine. The urine electrophoretic pattern in the sick dogs was classified as mixed (proteins with high and low molecular weights) because low molecular weight proteins made up 57.9% and the rest of the proteins had middle and high molecular weights. In Western blot, none of the healthy dogs showed excretion of IgG and/or IgA, whereas IgG and IgA were detected in the Western blot of urine of 68% and 55% respectively of dogs with leishmaniasis. The results obtained in the leishmaniasis group agreed with glomerular and tubular damage, which were confirmed by the histopathological findings. leishmaniasis / proteinuria / dog / SDS-PAGE / Western blot

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Analysis of the humoral immune response against total and recombinant antigens of Leishmania infantum: correlation with disease progression in canine experimental leishmaniasis

Cited by 101 publications

References 22 publications

Identification and Characterization of T Cell-Stimulating Antigens from Leishmania by CD4 T Cell Expression Cloning

Identification and Characterization of T Cell-Stimulating Antigens from Leishmania by CD4 T Cell Expression Cloning

Improving methods for epidemiological control of canine visceral leishmaniasis based on a mathematical model. Impact on the incidence of the canine and human disease

SDS-PAGE and Western blot of urinary proteins in dogs with leishmaniasis

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