Analysis of the <i>N</i>-Glycoforms and Immunoactivity of Chinese Yam (<i>Dioscorea opposita</i> Thunb.) Glycoprotein 30CYGP

Li, Cheng; Lu, Yu; Chen, Xiaoyi; Yang, Min; Zou, Zihua; Han, Jiaguang; Gao, Xi; Tang, Rui; Wang, Chengjian; Huang, Linjuan; Wang, Zhongfu

doi:10.1021/acs.jproteome.9b00102

Cited by 13 publications

(3 citation statements)

References 36 publications

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“…This experiment was performed as previously reported (Li et al, 2016(Li et al, , 2019. Briefly, 250 ll of water and 200 ll of protein denaturation solution were added to 2 mg of PD-1 proteins obtained from 293T cells, prior to heating at 100°C for 10 min.…”

Section: Release Of N-glycans and N-glycan Ms Analysismentioning

confidence: 99%

N‐glycosylation of PD‐1 promotes binding of camrelizumab

et al. 2020

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PD-1 is a highly glycosylated inhibitory receptor expressed mainly on T cells. Targeting of PD-1 with monoclonal antibodies (MAbs) to block the interaction with its ligand PD-L1 has been successful for the treatment of multiple tumors. However, polymorphisms at Nglycosylation sites of PD-1 exist in the human population that might affect antibody binding, and dysregulated glycosylation has been observed in the tumor microenvironment. Here, we demonstrate varied N-glycan composition in PD-1, and show that the binding affinity of camrelizumab, a recently approved PD-1-specific MAb, to non-glycosylated PD-1 proteins from E. coli is substantially decreased compared with glycosylated PD-1. The structure of the camrelizumab/PD-1 complex reveals that camrelizumab mainly utilizes its heavy chain to bind to PD-1, while the light chain sterically inhibits the binding of PD-L1 to PD-1. Glycosylation of asparagine 58 (N58) promotes the interaction with camrelizumab, while the efficiency of camrelizumab to inhibit the binding of PD-L1 is substantially reduced for glycosylation-deficient PD-1. These results increase our understanding of how glycosylation affects the activity of PD-1-specific MAbs during immune checkpoint therapy.

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Section: Release Of N-glycans and N-glycan Ms Analysismentioning

confidence: 99%

N‐glycosylation of PD‐1 promotes binding of camrelizumab

et al. 2020

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“…Research found that many compounds from Chinese yam (including dioscorin, polysaccharides and glycoprotein) had favorable immunomodulatory activity [44] . Also, the immunomodulatory properties of plant-based fermented foods were well documented [45][46][47] .…”

Section: Immunomodulatory Activities Of Fcyementioning

confidence: 99%

The metabolic profiling of Chinese yam fermented by Saccharomyces boulardii and the biological activities of its ethanol extract in vitro

Li,

Shao,

Hao

et al. 2024

Food Science and Human Wellness

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Chinese yam (Dioscorea opposita Thunb.), as one of the medicinal and edible homologous plants, is rich in various nutrients and functional factors. In this study, Chinese yam fermented by Saccharomyces boulardii was performed to investigate its bioactive components and metabolic profile. And then, the main bioactive components and biological activities of fermented Chinese yam ethanol extract (FCYE) were evaluated. Results showed that there were 49 up-regulated metabolites and 52 down-regulated metabolites in fermented Chinese yam compared to unfermented Chinese yam. Besides, corresponding metabolic pathways analysis initially revealed that the distribution of bioactive substances was concentrated on alcohol-soluble small molecular substances. Ulteriorly, the total polyphenol content and the total flavonoid content in FCYE were significantly increased, and the corresponding antioxidant and immunomodulatory activities in vitro were also significantly enhanced. Our study provided a new reference for the comprehensive utilization of Chinese yam and it also laid a theoretical foundation for the development and application of natural probiotic-fermented products.

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“…[1][2][3][4][5][6] Previous studies have indicated that D. opposita Thunb. extracts demonstrate anti-inflammatory effects, 7 antioxidant effects, 8 enable potential alleviation of diarrhea, 9 an influence on gut microbiota, 10 and immunomodulatory effects. 11 Wen County of Henan Province in China is the geo-authentic production base with thousands of years of cultivation history.…”

Section: Introductionmentioning

confidence: 99%

Unveiling the impact of harvest time on Dioscorea opposita Thunb. cv. Tiegun maturity by NMR‐based metabolomics and LC–MS/MS analysis

An,

Liu,

et al. 2024

J Sci Food Agric

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BackgroundDioscorea opposita Thunb. cv. Tiegun maturity (DM) is an important factor influencing its quality. However, there are few studies on the impact of harvest time on its maturation. In this study, a nuclear magnetic resonance (NMR)‐based metabolomics approach was applied to investigate the dynamic metabolic changes of Dioscorea opposita Thunb. cv. Tiegun at six different harvest stages of stage 1 (S1), stage 2 (S2), stage 3 (S3), stage 4 (S4), stage 5 (S5) and stage 6 (S6).ResultsPrincipal component analysis (PCA) showed distinct segregation of samples obtained from S1, S2, and S3 compared to those derived from S4, S5, and S6. Interestingly, these samples from the two periods were obtained before and after frost, indicating that frost descent might be important for DM. Eight differential metabolites responsible for good separation of different groups were identified by the PCA loading plot and partial least squares‐discriminant analysis (PLS‐DA). In addition, quantitative analysis of these metabolites using liquid chromatography–tandem mass spectrometry (LC–MS/MS) determined the effects of harvest time on these metabolite contents, two of which, sucrose and allantoin, were considered as potential biomarkers to determine DM.ConclusionThe work demonstrated that NMR‐based metabolomics approach could serve as powerful tool to identify differential metabolites during harvesting processes, which also offered a fresh insight for understanding the DM and the potential mechanism of quality formation.This article is protected by copyright. All rights reserved.

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Analysis of the N-Glycoforms and Immunoactivity of Chinese Yam (Dioscorea opposita Thunb.) Glycoprotein 30CYGP

Cited by 13 publications

References 36 publications

N‐glycosylation of PD‐1 promotes binding of camrelizumab

N‐glycosylation of PD‐1 promotes binding of camrelizumab

The metabolic profiling of Chinese yam fermented by Saccharomyces boulardii and the biological activities of its ethanol extract in vitro

Unveiling the impact of harvest time on Dioscorea opposita Thunb. cv. Tiegun maturity by NMR‐based metabolomics and LC–MS/MS analysis

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