Analysis of the immunoproteome of Mycoplasma mycoides subsp. mycoides small colony type reveals immunogenic homologues to other known virulence traits in related Mycoplasma species

Jores, Joerg; Meens, Jochen; Buettner, Falk F. R.; Linz, Bodo; Naessens, Jan; Gerlach, Gerald-F.

doi:10.1016/j.vetimm.2009.04.016

Cited by 42 publications

(56 citation statements)

References 32 publications

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“…Several studies employing different experimental approaches, e.g., in silico analysis, proteomics, cloning, etc., have described the identification of immunogenic Mycoplasma mycoides subsp. mycoides proteins (15)(16)(17)(18)20), yet as expected, each methodology had its limitation. This study is based on a thorough comparison of recombinant immunogens, using a standardized expression system employing synthetic, codon-optimized genes.…”

Section: Discussionmentioning

confidence: 80%

“…mycoides proteins included for evaluation in this study are listed in Table 1. Nine of the proteins were previously identified as immunogenic with diagnostic potential in collaborative studies (16,17). Eight additional proteins displaying strong serological responses and high disease specificity were also included (15,(18)(19)(20).…”

Section: Methodsmentioning

confidence: 99%

“…Previous research efforts have resulted in the identification of immunogenic Mycoplasma mycoides subsp. mycoides proteins with diagnostic potential, and a cocktail ELISA comprising eight selected mycoplasma antigens has been developed (15)(16)(17)(18). Although sensitive and specific, the rather large number of included recombinants may limit the production practicality of this assay in Africa.…”

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confidence: 99%

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Development of a Novel Cocktail Enzyme-Linked Immunosorbent Assay and a Field-Applicable Lateral-Flow Rapid Test for Diagnosis of Contagious Bovine Pleuropneumonia

et al. 2016

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e Contagious bovine pleuropneumonia (CBPP) is a severe respiratory disease that is widespread in sub-Saharan Africa. It is caused by Mycoplasma mycoides subsp. mycoides, a bacterium belonging to the Mycoplasma mycoides cluster. In the absence of an efficient CBPP vaccine, improved and easy-to-use diagnostic assays for recurrent testing combined with isolation and treatment of positive animals represent an option for CBPP control in Africa. Here we describe the comprehensive screening of 17 immunogenic Mycoplasma mycoides subsp. mycoides proteins using well-characterized bovine sera for the development of a novel cocktail enzyme-linked immunosorbent assay (ELISA) for laboratory use. Two recombinant Mycoplasma immunogens, MSC_0136 and MSC_0636, were used to set up a standardized cocktail ELISA protocol. According to the results from more than 100 serum samples tested, the sensitivity and specificity of the novel cocktail ELISA were 85.6% and 96.4%, respectively, with an overall diagnostic accuracy comparable to that of the Office International des Epizooties (OIE)-prescribed serological assays. In addition, we provide a proof of principle for a field-applicable, easy-to-use commercially produced prototype lateral-flow test for rapid (<30-min) diagnosis of CBPP.

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Section: Discussionmentioning

confidence: 80%

Section: Methodsmentioning

confidence: 99%

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confidence: 99%

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Development of a Novel Cocktail Enzyme-Linked Immunosorbent Assay and a Field-Applicable Lateral-Flow Rapid Test for Diagnosis of Contagious Bovine Pleuropneumonia

et al. 2016

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“…This is similar to findings for other mammals infected with mycoplasmas where housekeeper proteins, such as M. suis DnaK and glyceraldehyde-3-phosphate dehydrogenase and Mycoplasma mycoides subsp. mycoides small-colony DnaK, pgk, and elongation factors G and Tu were found to be immunogenic (3,4).…”

Section: Discussionmentioning

confidence: 99%

Detection of Humoral Response Using a Recombinant Heat Shock Protein 70, DnaK, ofMycoplasma haemofelisin Experimentally and Naturally Hemoplasma-Infected Cats

Barker

Helps

Heesom

et al. 2010

Clin Vaccine Immunol

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Hemoplasmas is the trivial name given to a group of erythrocyte-parasitizing bacteria of the genus Mycoplasma. Of the feline hemoplasmas, Mycoplasma haemofelis is the most pathogenic, while "Candidatus Mycoplasma haemominutum" and "Candidatus Mycoplasma turicensis" are less pathogenic. Shotgun libraries of fragmented M. haemofelis genomic DNA were constructed, and random colonies were selected for DNA sequencing. In silico-translated amino acid sequences of putative open reading frames were compared to mass spectrometry data from M. haemofelis protein spots identified as being immunogenic by two-dimensional gel electrophoresis and Western blotting. Three of the spots matched the predicted sequences of a heat shock protein 70 (DnaK) homolog, elongation factor Ts, and a fragment of phosphoglycerate kinase found during library screening. A full-length copy of the M. haemofelis dnaK gene was cloned into Escherichia coli and recombinantly expressed. Recombinant M. haemofelis DnaK was purified and then used in Western blotting and an enzyme-linked immunosorbent assay (ELISA) to investigate the humoral immune response during acute infection in cats experimentally infected with M. haemofelis, "Ca. Mycoplasma haemominutum," or "Ca. Mycoplasma turicensis". The recombinant M. haemofelis DnaK ELISA also was used to screen clinical samples submitted for hemoplasma PCR testing to a commercial laboratory (n ‫؍‬ 254). Experimentally infected cats became seropositive following infection, with a greater and earlier antibody response seen in cats inoculated with M. haemofelis than those seen in cats inoculated with "Ca. Mycoplasma haemominutum" or "Ca. Mycoplasma turicensis," by both Western blotting and ELISA. Of the clinical samples, 31.1% had antibodies detected by the ELISA but only 9.8% were positive by PCR for one or more hemoplasmas.

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“…Unexpectedly, fructose-bisphosphate aldolase class II, TPI, PGK, GAPDH, phosphoglyceromutase (GPM), enolase, glucose-6-phosphate isomerase, and L-lactate dehydrogenase of Mycoplasma mycoides subsp. mycoides small colony type (MmmSC) were identified as novel immunogenic proteins by immunoblot analysis with sera from MmmSC-infected animals and MALDI-ToF mass spectrometry [37]. Furthermore, the bactericidal assay using mouse antiserum killed 70.55% of M. gallisepticum R low cells.…”

Section: A N U S C R I P Tmentioning

confidence: 99%

Expression and immunological characteristics of the surface-localized pyruvate kinase in Mycoplasma gallisepticum

et al. 2015

Microbial Pathogenesis

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Analysis of the immunoproteome of Mycoplasma mycoides subsp. mycoides small colony type reveals immunogenic homologues to other known virulence traits in related Mycoplasma species

Cited by 42 publications

References 32 publications

Development of a Novel Cocktail Enzyme-Linked Immunosorbent Assay and a Field-Applicable Lateral-Flow Rapid Test for Diagnosis of Contagious Bovine Pleuropneumonia

Development of a Novel Cocktail Enzyme-Linked Immunosorbent Assay and a Field-Applicable Lateral-Flow Rapid Test for Diagnosis of Contagious Bovine Pleuropneumonia

Detection of Humoral Response Using a Recombinant Heat Shock Protein 70, DnaK, ofMycoplasma haemofelisin Experimentally and Naturally Hemoplasma-Infected Cats

Expression and immunological characteristics of the surface-localized pyruvate kinase in Mycoplasma gallisepticum

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