The dynamic load generated by traffic has a negative impact not only on human health and a well-being, but also on the lifetime of building structures. The article deals with the proposal of the prognostic vibro-acoustic analysis methodology of the building of the Slovak National Theatre, which, based on real vibro-acoustic measurements on a similar elevated building structure and potential sources of excitation, was applied to the premises of the cultural stand. The article also analyses the impact of vibration transmission from a potential source, which is a rail vehicle, on the generation of low-frequency noise in acoustically protected spaces.