Analysis of the inflammatory response induced by rhMMP-12 catalytic domain instilled in mouse airways

Nénan, Soazig; Planquois, Jean-Michel; Berna, Patrick; Mendez, I de; Hitier, Simon; Shapiro, Steve D.; Boichot, Elisabeth; Lagente, Vincent; Bertrand, Claude

doi:10.1016/j.intimp.2004.10.011

Cited by 47 publications

(36 citation statements)

References 50 publications

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“…37) On the other hand, intratracheal instillation of recombinant human MMP-12 can induce airway inflammation in naive mice. 35) Thus, MMP-12 might be one of the important mediators in the pathogenesis of allergic airway inflammation.…”

Section: Discussionmentioning

confidence: 99%

Increase in the Expression of Matrix Metalloproteinase-12 in the Airways of Rats with Allergic Bronchial Asthma

Chiba

Sakai

et al. 2007

Biological & Pharmaceutical Bulletin

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Matrix metalloproteinases (MMPs) are a family of proteinases which have zinc dependent structure centre, widely degrade extracellular matrix components and cleave non-matrix proteins including growth factors, chemoattractants and cell surface receptors.1) Most of the MMPs contain pro-domain which is accordant, through binding of the cysteine of pro-domain and histidines of the catalytic domain, to the metallo cation Zn 2ϩ . MMPs are activated by cleavage to set the catalytic domain free to act on substrates.MMP-12, which is also called metalloelastase and macrophage elastase, was firstly detected in mouse peritoneal macrophage.2) Subsequently, it was also found in alveolar macrophages, 3,4) bronchial epithelial cells 5) and airway smooth muscle cells. 6) Like other MMPs, MMP-12 consists of a pro-domain, a catalytic domain and a hemopexin domain, and is activated by taking off the pro-domain and then the hemopexin domain remaining the catalytic domain. It has been reported that the rat MMP-12 catalytic domain can degrade collagen-V and partially degrade collagen-I 7) and other extracellular matrix proteins. 8)Although the role of MMP-12 in airways is not fully understood, an involvement of MMP-12 has been reported in cigarette smoking-associated airway inflammatory diseases, such as chronic obstructive pulmonary disease (COPD). 9)MMP-12 has been reported to be associated with cigarette smoke-induced emphysema 10) and macrophage migration. 11)In patients with COPD, MMP-12-positive macrophages were increased in airway epithelial layers and bronchoalveolar lavage fluids when compared with normal subjects. 12) On the other hand, an upregulation of MMP-12 was also found in a mouse model of airway inflammation and remodeling induced by IL-13.13) Furthermore, an increased expression of MMP-12 was reported in a mouse model of allergic airway inflammation: the MMP-12 upregulation was not observed in IL-13-knockout mice.14) It is thus possible that MMP-12 is also relating to airway diseases mediated by Th2 cytokines such as allergic bronchial asthma. Indeed, MMP-12 has been proposed as one of the asthma candidate genes. 15) In the present study, the changes in the expression and localization of MMP-12 in airways of repeatedly antigen-challenged rats were investigated to reveal whether or not MMP-12 has relevance to allergic bronchial asthma. MATERIALS AND METHODS Sensitization and Antigenic ChallengeMale Wistar rats (6 weeks of age, specific pathogen-free, 170-190 g; Charles River Japan, Inc.) were sensitized and repeatedly challenged with 2,4-dinitrophenylated Ascaris suum antigen (DNP-Asc) by the method described in the previous papers. [16][17][18][19][20][21][22][23][24] In brief, the rats were sensitized with DNP-Asc together with Bordetella pertussis and were boosted 5 d later. Eight days after the first immunization, the rats were challenged by inhaling DNP-Asc for 40 min under conscious state. Then the animals were subjected to totally three times repeated antigen challenge every 48 h with the same inhalational cha...

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Section: Discussionmentioning

confidence: 99%

Increase in the Expression of Matrix Metalloproteinase-12 in the Airways of Rats with Allergic Bronchial Asthma

Chiba

Sakai

et al. 2007

Biological & Pharmaceutical Bulletin

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“…A single instillation of rhMMP-12 into the mouse airways elicited an intense inflammatory response characterized by the development of two successive phases. Indeed, rhMMP-12 induced an acute, severe and specific recruitment of neutrophils reaching a peak at 18 h, while the number of macrophages remained stable throughout this period (30). The non-selective MMP inhibitor, marimastat, dexamethasone and the PDE4 inhibitor, rolipram, administered orally 1 h before rhMMP-12 instillation, were able to significantly decrease neutrophil recruitment at 4 and 24 h. However, only marimastat was effective regarding macrophage recruitment at day 7.…”

Section: Role Of Mmp In the Development Of Airway Inflammation In Chrmentioning

confidence: 95%

Role of matrix metalloproteinases in the development of airway inflammation and remodeling

Lagente

Manoury

Nénan

et al. 2005

Braz J Med Biol Res

147

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“…It was reported that rhMMP-12 catalytic domain, directly injected in the airways of mice, is able to induce an intense inflammatory response characterized by a rapid accumulation of neutrophils correlated to a transient increase in cytokines and chemokines levels (TNF-a, MIP-1a, MCP-1, IL-6, and KC) and to gelatinase activation. A later response was also observed mainly due to macrophage recruitment (Nenan et al, 2005). The effect of rhMMP-12 was also examined on human alveolar type II-like epithelial cells (A549) and on human bronchial epithelial cells (BEAS-2B), showing that rhMMP-12 enhances the release of several chemokines by A549 cells, in particular, monocyte chemoattractant protein-1 (MCP-1)/CCL2, growth-related oncogene-a (GRO-a)/CXCL1, and IL-8/CXCL8 (Le Quement et al, 2008).…”

Section: Spinal Cord Injury (Sci)mentioning

confidence: 96%

Human matrix metalloproteinases: An ubiquitarian class of enzymes involved in several pathological processes

Sbardella

Fasciglione

Gioia

et al. 2012

Molecular Aspects of Medicine

209

212

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a b s t r a c tHuman matrix metalloproteinases (MMPs) belong to the M10 family of the MA clan of endopeptidases. They are ubiquitarian enzymes, structurally characterized by an active site where a Zn 2+ atom, coordinated by three histidines, plays the catalytic role, assisted by a glutamic acid as a general base. Various MMPs display different domain composition, which is very important for macromolecular substrates recognition. Substrate specificity is very different among MMPs, being often associated to their cellular compartmentalization and/or cellular type where they are expressed. An extensive review of the different MMPs structural and functional features is integrated with their pathological role in several types of diseases, spanning from cancer to cardiovascular diseases and to neurodegeneration. It emerges a very complex and crucial role played by these enzymes in many physiological and pathological processes.

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Analysis of the inflammatory response induced by rhMMP-12 catalytic domain instilled in mouse airways

Cited by 47 publications

References 50 publications

Increase in the Expression of Matrix Metalloproteinase-12 in the Airways of Rats with Allergic Bronchial Asthma

Increase in the Expression of Matrix Metalloproteinase-12 in the Airways of Rats with Allergic Bronchial Asthma

Role of matrix metalloproteinases in the development of airway inflammation and remodeling

Human matrix metalloproteinases: An ubiquitarian class of enzymes involved in several pathological processes

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