The 3D printing process allows complex structures to be obtained with low environmental impact using biodegradable materials. This work aims to develop and acoustically characterize 3D-printed panels using three types of materials, each manufactured at five infill densities (20%, 40%, 60%, 80% and 100%) with three internal configurations based on circular, triangular, and corrugated profiles. The highest absorption coefficient values (α = 0.93) were obtained from the acoustic tests for the polylactic acid material with ground birch wood particles in the triangular configuration with an infill density of 40%. The triangular profile showed the best acoustic performance for the three types of materials analysed and, from the point of view of the mechanical tests, it was highlighted that the same triangular configuration presented the highest resistance both to compression (40 MPa) and to three-point bending (50 MPa). The 40% and 60% infill density gave the highest absorption coefficient values regardless of the material analyzed. The mechanical tests for compression and three-point bending showed higher strength values for samples manufactured from simple polylactic acid filament compared to samples manufactured from ground wood particles. The standard defects of 3D printing and the failure modes of the interior configurations of the 3D-printed samples could be observed from the microscopic analysis of the panels. Based on the acoustic results and the determined mechanical properties, one application area for these types of 3D-printed panels could be the automotive and aerospace industries.