Analysis of the influence of drug resistance factors on the efficacy of combinations of antibiotics for multidrug-resistant Pseudomonas aeruginosa isolated from hospitals located in the suburbs of Kanto area, Japan

Kataoka, Hiroshi; Ida, Takashi; Ishii, Yoshikazu; Tateda, Kazuhiro; Oguri, Toyoko; Yoshida, Atsushi; Okuzumi, Katsuko; Oishi, Tsuyoshi; Tsukahara, Miyuki; Mori, Shinichiro; Yoneyama, Akio; Araoka, Hideki; Mitsuda, Toshihiro; Sumitomo, Midori; Moriya, Kyoji; Goto, Mieko; Nakamori, Yoshitaka; Shibayama, Akiyoshi; Ohmagari, Norio; Sato, Tomoaki; Yamaguchi, Keizo

doi:10.1016/j.jgar.2013.03.005

Cited by 16 publications

(10 citation statements)

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“…In Japan, as many as 80-90% of the MBL-producing P. aeruginosa were reported to be resistant to carbapenems, aminoglycosides, and quinolones [ 14 , 15 ]. Notably, several molecular epidemiological surveys report the increasing prevalence of multidrug-resistant P. aeruginosa (MDRP); in these the predominance of IMP-type MBL genes as well as ST235 and ST357 among MDRP is documented [ 15 - 20 ].…”

Section: Introductionmentioning

confidence: 99%

Molecular analysis of the integrons of metallo-β-lactamase-producing Pseudomonas aeruginosa isolates collected by nationwide surveillance programs across Japan

et al. 2015

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BackgroundWe investigate the evolving molecular epidemiology of metallo-β-lactamase (MBL)-producing Pseudomonas aeruginosa isolates collected in a 100 institution, nationwide surveillance study in Japan from 2004 to 2006.ResultsMBL-producers were detected in 23/996 isolates (2.3%) in 2004 and 21/992 (2.1%) in 2006. Antimicrobial resistance (specifically, carbapenem resistance) rates between two periods did not differ significantly. MBL-producers were more prevalent in urinary tract isolates. blaIMP-1 group was the most predominant (38 isolates, 80%), followed by 3 blaIMP-7, 2 blaIMP-11 group, and 1 blaVIM-1. All MBL genes were identified in 16 different class 1 integrons, most of which were novel to INTEGRALL database. A total of 17 isolates of sequence type (ST) 235, a recognized worldwide drug-resistant lineage, were distributed in 5 geographic regions across Japan. ST235 isolates included a sublineage associated with In113-like integron. ST357 was identified in 14 isolates, 9 of which harboring a sole blaIMP-1 gene cassette (In994) were recovered from Chugoku region in 2004. ST357 isolates with blaIMP-11 group or ST235 with blaIMP-7 emerged in 2006. We also report for the first time the presence of novel fosI gene cassette in strains other than Mycobacterium spp.ConclusionsOur data give an important “snapshot” of the molecular characteristics and dynamics of MBL-producing lineages in P. aeruginosa in Japan. The significant association of specific genotypes and integrons implies that dissemination and transmission of the preexisting resistant lineage, rather than horizontal gene transfer in situ, might largely explain their endemicity.

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Section: Introductionmentioning

confidence: 99%

Molecular analysis of the integrons of metallo-β-lactamase-producing Pseudomonas aeruginosa isolates collected by nationwide surveillance programs across Japan

et al. 2015

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“…Antibiotic combination therapy study groups also reported that a combination of ABK plus AZT showed synergistic effects as well as the combinations of CL plus rifampin, and AMK plus AZT ( Figure 6 ). 21 These results suggest that ABK is a useful agent for MDRP infections used in combination therapies.…”

Section: Antibacterial Activities Against Multidrug-resistant mentioning

confidence: 89%

Arbekacin: another novel agent for treating infections due to methicillin-resistant Staphylococcus aureus and multidrug-resistant Gram-negative pathogens

Matsumoto¹

2014

CPAA

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Arbekacin sulfate (ABK), an aminoglycoside antibiotic, was discovered in 1972 and was derived from dibekacin to stabilize many common aminoglycoside modifying enzymes. ABK shows broad antimicrobial activities against not only Gram-positive bacteria including methicillin-resistant Staphylococcus aureus (MRSA) but also Gram-negative bacteria such as Escherichia coli, Pseudomonas aeruginosa, and Klebsiella pneumoniae. ABK has been approved as an injectable formulation in Japan since 1990, under the trade name Habekacin, for the treatment of patients with pneumonia and sepsis caused by MRSA. The drug has been used in more than 250,000 patients, and its clinical benefit and safety have been proven over two decades. ABK currently shows promise for the application for the treatment of multidrug-resistant Gram-negative bacterial infections such as multidrug-resistant strains of P. aeruginosa and Acinetobacter baumannii because of its synergistic effect in combination with beta-lactams.

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“…We also examined the growth inhibition of multidrugresistant P. aeruginosa. Importantly, CoOEOP-HasA effectively inhibited the growth of multidrug-resistant P. aeruginosa (MDRP0043 [14] ) (Figure 3(b)), which has been declared a hazardous bacterium by the WHO. [15] Thus, the use of reconstructed proteins with heteroporphyrins shows promising potential to combat multidrug-resistant P. aeruginosa.…”

Section: Methodsmentioning

confidence: 99%

A Heme‐Acquisition Protein Reconstructed with a Cobalt 5‐Oxaporphyrinium Cation and Its Growth‐Inhibition Activity Toward Multidrug‐Resistant Pseudomonas aeruginosa

et al. 2022

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Using artificial hemes for the reconstruction of natural heme proteins represents a fascinating approach to enhance the bioactivity of the latter. We report the synthesis of various metal 5-oxaporphyrinium cations as cofactors, and a cobalt 5-oxaporphyrinium cation was successfully incorporated into the heme-acquisition protein (HasA) secreted by Pseudomonas aeruginosa. We hypothesize that the oxaporphyrinium cation strongly binds to the HasA-specific outer membrane receptor (HasR) due to its cationic charge, which prevents the subsequent acquisition of heme. In fact, the reconstructed HasA inhibited the growth of Pseudomonas aeruginosa and even of multidrug-resistant P. aeruginosa.

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Analysis of the influence of drug resistance factors on the efficacy of combinations of antibiotics for multidrug-resistant Pseudomonas aeruginosa isolated from hospitals located in the suburbs of Kanto area, Japan

Cited by 16 publications

References 20 publications

Molecular analysis of the integrons of metallo-β-lactamase-producing Pseudomonas aeruginosa isolates collected by nationwide surveillance programs across Japan

Molecular analysis of the integrons of metallo-β-lactamase-producing Pseudomonas aeruginosa isolates collected by nationwide surveillance programs across Japan

Arbekacin: another novel agent for treating infections due to methicillin-resistant Staphylococcus aureus and multidrug-resistant Gram-negative pathogens

A Heme‐Acquisition Protein Reconstructed with a Cobalt 5‐Oxaporphyrinium Cation and Its Growth‐Inhibition Activity Toward Multidrug‐Resistant Pseudomonas aeruginosa

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