2017
DOI: 10.1371/journal.pntd.0006089
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Analysis of the interactome of Schistosoma mansoni histone deacetylase 8

Abstract: BackgroundHistone deacetylase 8 from Schistosoma mansoni (SmHDAC8) is essential to parasite growth and development within the mammalian host and is under investigation as a target for the development of selective inhibitors as novel schistosomicidal drugs. Although some protein substrates and protein partners of human HDAC8 have been characterized, notably indicating a role in the function of the cohesin complex, nothing is known of the partners and biological function of SmHDAC8.Methodology/Principal findings… Show more

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Cited by 9 publications
(14 citation statements)
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“…This gene has been linked to normal egg production and number of eggs produced [20], and our EBi3 -/-I mice eliminated smaller quantities of eggs in feces. SmMBD forms part of the NuRD repression complex along with the HDAC8 enzyme [42]. In our study, this machinery was downregulated in male parasites recovered from EBi3 -/-I mice due to decreased SmMBD expression; SmHDAC8 expression levels revealed only a loss of sex-specific function in worms recovered from EBi3 -/-I mice.…”
Section: Discussionmentioning
confidence: 52%
“…This gene has been linked to normal egg production and number of eggs produced [20], and our EBi3 -/-I mice eliminated smaller quantities of eggs in feces. SmMBD forms part of the NuRD repression complex along with the HDAC8 enzyme [42]. In our study, this machinery was downregulated in male parasites recovered from EBi3 -/-I mice due to decreased SmMBD expression; SmHDAC8 expression levels revealed only a loss of sex-specific function in worms recovered from EBi3 -/-I mice.…”
Section: Discussionmentioning
confidence: 52%
“…The SmRho family was further characterized by Vermeire and coworkers [19] and they identified S. mansoni orthologs of RhoA, CDC42 and Rac1. Interestingly, using the yeast two-hybrid methodology and mass spectrometry analysis, we recently identified the S. mansoni Ras homolog 1 (SmRho1), as a SmHDAC8 partner protein [15]. Moreover, after a more detailed reconstruction of the SmRho1 gene using RT-PCR, we discovered that two distinct genes encode Rho1 isoforms: Rho1.1 and 1.2 .…”
Section: Resultsmentioning
confidence: 99%
“…To confirm in vivo the direct interaction between SmHDAC8 and SmRho1 shown by Y2H screening [15], we performed co-immunoprecipitation (CoIP) experiments on adult worms (Fig 4A) and schistosomula (Fig 4B). Using anti-SmHDAC8 and SmRho1 antibodies, endogenous proteins were reciprocally and mutually immunoprecipitated and identified by Western blotting.…”
Section: Resultsmentioning
confidence: 99%
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