Analysis of the MAT1-2-1 gene of Colletotrichum lindemuthianum

García-Serrano, Mónica; Laguna, Emigdia Alfaro; Rodríguez-Guerra, Raúl; Simpson, June

doi:10.1007/s10267-008-0424-6

Cited by 16 publications

(15 citation statements)

References 33 publications

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“…Separation of a small subset of the strains into different groups by random amplified polymorphic DNA (RAPD) fingerprinting has been reported in earlier studies [5], [62]. In the current study, primers designed to amplify the HMG region of the MAT1-2-1 gene of C. lindemuthianum [10] failed to amplify any of the Glomerella strains, although they successfully amplified the expected fragment from a large collection of asexual, pathogenic C. lindemuthianum strains from the same regions of Brazil [33]. A primer pair specific for the HMG sequence of the Glomerella strains was developed by first amplifying the region using degenerate universal primers [42].…”

Section: Discussionmentioning

confidence: 74%

Characterization of Glomerella Strains Recovered from Anthracnose Lesions on Common Bean Plants in Brazil

et al. 2014

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Anthracnose caused by Colletotrichum lindemuthianum is an important disease of common bean, resulting in major economic losses worldwide. Genetic diversity of the C. lindemuthianum population contributes to its ability to adapt rapidly to new sources of host resistance. The origin of this diversity is unknown, but sexual recombination, via the Glomerella teleomorph, is one possibility. This study tested the hypothesis that Glomerella strains that are frequently recovered from bean anthracnose lesions represent the teleomorph of C. lindemuthianum. A large collection of Glomerella isolates could be separated into two groups based on phylogenetic analysis, morphology, and pathogenicity to beans. Both groups were unrelated to C. lindemuthianum. One group clustered with the C. gloeosporioides species complex and produced mild symptoms on bean tissues. The other group, which belonged to a clade that included the cucurbit anthracnose pathogen C. magna, caused no symptoms. Individual ascospores recovered from Glomerella perithecia gave rise to either fertile (perithecial) or infertile (conidial) colonies. Some pairings of perithecial and conidial strains resulted in induced homothallism in the conidial partner, while others led to apparent heterothallic matings. Pairings involving two perithecial, or two conidial, colonies produced neither outcome. Conidia efficiently formed conidial anastomosis tubes (CATs), but ascospores never formed CATs. The Glomerella strains formed appressoria and hyphae on the plant surface, but did not penetrate or form infection structures within the tissues. Their behavior was similar whether the beans were susceptible or resistant to anthracnose. These same Glomerella strains produced thick intracellular hyphae, and eventually acervuli, if host cell death was induced. When Glomerella was co-inoculated with C. lindemuthianum, it readily invaded anthracnose lesions. Thus, the hypothesis was not supported: Glomerella strains from anthracnose lesions do not represent the teleomorphic phase of C. lindemuthianum, and instead appear to be bean epiphytes that opportunistically invade and sporulate in the lesions.

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Section: Discussionmentioning

confidence: 74%

Characterization of Glomerella Strains Recovered from Anthracnose Lesions on Common Bean Plants in Brazil

et al. 2014

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“…Except for Colletotrichum (Cisar and TeBeest, 1999;García-Serrano et al, 2008;Menat et al, 2012) and Hymenoscyphus (Gross et al, 2012), both MAT1-1-1 and MAT1-2-1 have been successfully identified in almost every attempt to do so among members of Ascomycota to date. This supports the hypothesis that these genes are generally required for mating , although the two genes may have additional functions or differ in relative importance (Böhm et al, 2013;Yun et al, 2013).…”

Section: Mat1-2-1 Was the Only Spliced Transcript Detected Among The mentioning

confidence: 97%

Characterization and distribution of mating-type genes of the turfgrass pathogen Sclerotinia homoeocarpa on a global scale

Putman

Tredway

Carbone

2015

Fungal Genetics and Biology

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“…A pair of primers specific to C. lindemuthianum were used, namely HMGCLF and HMGCLR, which were described by García-Serrano et al (2008) and amplify the HMG region of the matingtype gene MAT1-2-1. Because these strains belong to the group of Glomerella sp that do not generate amplification bands when tested with primers specific to C. lindemuthianum as described above, a pair of primers specific to these strains of Glomerella sp, HMGGLOF and HMGGLOR, were used (Barcelos et al, 2011).…”

Section: Amplification Of the Hmg Region Of The Mating-type Gene Mat1mentioning

confidence: 99%

“…The HMG region of the mating-type gene MAT1-2-1 only amplified in strains of the C. lindemuthianum group (García-Serrano et al, 2008). When primer pairs specific for the HMG region of the mating-type gene MAT1-2-1 were tested (Barcelos et al, 2011), the expected product amplified only in G7-2, G8-1, G15-5, G25-1, G43-2, and G68-1.…”

Section: Amplification Of the Hmg Region Of The Mating-type Genementioning

confidence: 99%

Variability of Colletotrichum spp in common bean

Mota¹,

Barcelos²,

Dias³

et al. 2016

Genet. Mol. Res.

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The Colletotrichum genus presents large genetic variability, as demonstrated by the occurrence of several pathogenic races and phenotypic traits. The objective of this study was to characterize 22 strains of C. lindemuthianum and Colletotrichum spp recovered from anthracnose lesions and bean scab, and to verify the relationship between species of the Colletotrichum genus, which inhabit anthracnose and scab lesions. Colony morphology, conidium size, the presence of septa, germination, sporulation, and mycelium growth rates, were analyzed in addition to the presence of mating-type genes, IRAP markers, and pathogenicity. Strains of Colletotrichum spp presented wide variation for all evaluated traits, indicating the presence of different species. Pathogenicity tests verified that the severity of the disease caused by strains of Colletotrichum spp must be evaluated 17 days after inoculation. Molecular analysis showed that only the C. lindemuthianum strains were grouped by the IRAP markers. For the physiological traits, we observed that C. lindemuthianum mycelium growth is slower than that of Colletotrichum spp strains. The information generated in this study confirms variability in the evaluated species of Colletotrichum and may direct future basic and applied studies aiming to control these diseases in common bean.

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Analysis of the MAT1-2-1 gene of Colletotrichum lindemuthianum

Cited by 16 publications

References 33 publications

Characterization of Glomerella Strains Recovered from Anthracnose Lesions on Common Bean Plants in Brazil

Characterization of Glomerella Strains Recovered from Anthracnose Lesions on Common Bean Plants in Brazil

Characterization and distribution of mating-type genes of the turfgrass pathogen Sclerotinia homoeocarpa on a global scale

Variability of Colletotrichum spp in common bean

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