Analysis of the mechanism of discrepant nuclear morphometric results comparing preoperative biopsy and prostatectomy specimens

Kanamaru, Hiroshi; Zhang, Yan Hu; Takahashi, Masahiko; Nakamura, Naohiro; Ishida, Hirokazu; Akino, Hironobu; Muranaka, K; Okada, Kenichiro

doi:10.1016/s0090-4295(00)00583-5

Cited by 4 publications

(2 citation statements)

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“…We have previously reported that the results of nuclear morphometric measurement (nuclear size and shape) of prostatic tissue could be seriously distorted during the postoperative processing of surgical specimens 24,25 . We therefore hypothesized that the incorporation of an internal standard (normal cell nuclei) could minimize the effect of such tissue processing artifacts, and we proposed a new morphometric parameter, NAI, which is defined as the ratio of MNA for cancer cells to MNA for normal cells.…”

Section: Discussionmentioning

confidence: 99%

Prognostic value of nuclear area index in patients with bladder cancer

et al. 2004

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Background : To assess the prognostic usefulness of the nuclear area index (NAI), a new nuclear morphometric parameter expressed as the mean nuclear area (MNA) ratio of cancer to normal transitional cells in patients with bladder cancer, who have undergone radical cystectomy. Methods : Measurements of the nuclear areas of cancer and normal transitional cells were carried out on the histological slides of 73 patients with bladder cancer. The clinical usefulness of MNA, NAI, grade, and TNM categories for the prediction of the cause-specific survival of the patients was examined.Results : The median values of MNA and NAI in the 73 patients were 39 m m 2 and 1.2, respectively. Cause-specific survival rates of the patients were calculated according to stage (T1-2 vs T3-4), grade (grade 2 vs grade 3), MNA ( < 39 m m 2 vs ≥ 39 m m 2 ) and NAI value ( < 1.2 vs ≥ 1.2). Using univariate analysis, all these parameters were statistically significant prognostic factors. However, by multivariate analysis, NAI was the only independent variable for the survival of the patients ( P < 0.01). Causespecific survival rates of patients with NAI values of less than 1.2 were significantly higher than those with NAI values of 1.2 or more, in both grade 2 and grade 3 tumors. Conclusions : These results suggest that NAI could provide improved prognostic information for patients with bladder cancer.

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Section: Discussionmentioning

confidence: 99%

Prognostic value of nuclear area index in patients with bladder cancer

et al. 2004

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“…However, it is fundamental in morphometric studies of the nuclei of prostatic epithelial cells, that there is an initial immediate fixation of the material (27), as it occurred in the present study.…”

Section: Commentsmentioning

confidence: 96%

Bladder drainage and glandular epithelial morphometry of the prostate in benign prostatic hyperplasia with severe symptoms

2006

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Objective: Morphometrically analyze the cells nuclei of the basal layer of the prostatic glandular epithelium in 20 patients aged between 57 and 85 years presenting benign prostatic hyperplasia with severe symptoms, catheterized or not. Materials and Methods:Patients with score of severe prostatic symptoms (with indication for transurethral resection of the prostate) were distributed according to the presence or absence of bladder drainage previous to the surgery, in the treated group (n = 10, catheter during 3 months) and in the control group (n = 10, without catheter). After obtaining prostate fragments through transurethral resection and the use of morphometric techniques, 100 nuclei of prostatic glands epithelium cells were studied (as to size and form), and compared to 500 nuclei from patients submitted to catheter drainage and 500 nuclei of non-catheterized patients. Results:Significantly reduced values of the major, medium and minor nuclear diameters, volume, area and perimeter, contour index and nuclear volume-nuclear area ratio were observed in the treated group in relation to the control group. As to the form, eccentricity and coefficient of nuclear form, there were significant differences between treated and control groups. Conclusion: Long-term catheter bladder drainage in patients presenting benign prostatic hyperplasia with severe symptoms is associated to the reduction of morphometric parameters of the nuclei of prostatic glands' epithelial cells, suggesting a likely decompressive duct effect.

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