Analysis of the Mechanisms Underlying the Specificity of the Variation Potential Induced by Different Stimuli

Abstract

Plants are able to perceive diverse environmental factors and form an appropriate systemic functional response. Systemic responses are induced by stimulus-specific long-distance signals that carry information about the stimulus. Variation potential is proposed as a candidate for the role of such a signal. Here, we focus on the mechanisms that determine the specificity of the variation potential under the action of different local stimuli. Local stimuli such as heating, burning and wounding cause variation potential, the parameters of which differ depending on the type of stimulus. It was found that the stimulus-specific features of the hydraulic signal monitored by changes in leaf thickness and variation potential, such as a greater amplitude upon heating and burning and a significant amplitude decrement upon burning and wounding, were similar. The main features of these signals are the greater amplitude upon heating and burning, and a significant amplitude decrement upon burning and wounding. Together with the temporal correspondence of signal propagation, this evidence indicates a role for the hydraulic signal in the induction of stimulus-specific variation potential. Experiments using mechanosensitive channel inhibitors have demonstrated that the hydraulic signal contributes more to the induction of the variation potential in the case of rapidly growing stimuli, such as burning and wounding, than in the case of gradual heating. For thermal stimuli (gradual heating and burning), a greater contribution, compared to wounding, of the chemical signal related to reactive oxygen species to the induction of the variation potential was demonstrated. Thus, the specificity of the parameters of the variation potential is determined by the different contributions of hydraulic and chemical signals.