Analysis of the meiotic role of the mitochondrial ribosomal proteins Mrps17 and Mrpl37 in <i>Saccharomyces cerevisiae</i>

Hanlon, Sean E.; Xu, Zhiheng; Norris, David; Vershon, Andrew K.

doi:10.1002/yea.1174

Cited by 7 publications

(5 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Possible extraribosomal functions have to date been found for three of the yeast mitochondrial ribosomal proteins only. Mrps17p and Mrpl37p may play a special role in yeast sporulation [33]. The mitochondrial ribosomal protein of the small subunit, yDAP-3 [34] is well conserved between yeast and human cells and besides its translational role has a distinct function in apoptosis.…”

Section: Discussionmentioning

confidence: 99%

The mitochondrial ribosomal protein of the large subunit, Afo1p, determines cellular longevity through mitochondrial back-signaling via TOR1

Heeren

Rinnerthaler

Laun

et al. 2009

Aging

View full text Add to dashboard Cite

Yeast mother cell-specific aging constitutes a model of replicative aging as it occurs in stem cell populations of higher eukaryotes. Here, we present a new long-lived yeast deletion mutation,afo1 (for aging factor one), that confers a 60% increase in replicative lifespan. AFO1/MRPL25 codes for a protein that is contained in the large subunit of the mitochondrial ribosome. Double mutant experiments indicate that the longevity-increasing action of the afo1 mutation is independent of mitochondrial translation, yet involves the cytoplasmic Tor1p as well as the growth-controlling transcription factor Sfp1p. In their final cell cycle, the long-lived mutant cells do show the phenotypes of yeast apoptosis indicating that the longevity of the mutant is not caused by an inability to undergo programmed cell death. Furthermore, the afo1 mutation displays high resistance against oxidants. Despite the respiratory deficiency the mutant has paradoxical increase in growth rate compared to generic petite mutants. A comparison of the single and double mutant strains for afo1 and fob1 shows that the longevity phenotype of afo1 is independent of the formation of ERCs (ribosomal DNA minicircles). AFO1/MRPL25 function establishes a new connection between mitochondria, metabolism and aging.

show abstract

Section: Discussionmentioning

confidence: 99%

The mitochondrial ribosomal protein of the large subunit, Afo1p, determines cellular longevity through mitochondrial back-signaling via TOR1

Heeren

Rinnerthaler

Laun

et al. 2009

Aging

View full text Add to dashboard Cite

show abstract

“…Nonsynonymous amino acid substitutions identified in Mrpl7, Hem13, and Ppa2 are predicted by mutfunc 17 to have functional impacts as they affect conserved amino acids (Supporting Information Table S3 ). Mrpl37 works as a component of the mitochondrial ribosome 77 and its Arg89Ser substitution is predicted by NetPhosYeast 15 to introduce a phosphorylation site, with Ste7 as the predicted corresponding kinase. The mechanisms through which this phosphorylation site introduction aids in acquiring ethanol tolerance remain to be tackled.…”

Section: Resultsmentioning

confidence: 99%

Integrated Multi-Omics Analysis of Mechanisms Underlying Yeast Ethanol Tolerance

et al. 2021

View full text Add to dashboard Cite

For yeast cells, tolerance to high levels of ethanol is vital both in their natural environment and in industrially relevant conditions. We recently genotyped experimentally evolved yeast strains adapted to high levels of ethanol and identified mutations linked to ethanol tolerance. In this study, by integrating genomic sequencing data with quantitative proteomics profiles from six evolved strains (data set identifier PXD006631) and construction of protein interaction networks, we elucidate exactly how the genotype and phenotype are related at the molecular level. Our multi-omics approach points to the rewiring of numerous metabolic pathways affected by genomic and proteomic level changes, from energy-producing and lipid pathways to differential regulation of transposons and proteins involved in cell cycle progression. One of the key differences is found in the energy-producing metabolism, where the ancestral yeast strain responds to ethanol by switching to respiration and employing the mitochondrial electron transport chain. In contrast, the ethanol-adapted strains appear to have returned back to energy production mainly via glycolysis and ethanol fermentation, as supported by genomic and proteomic level changes. This work is relevant for synthetic biology where systems need to function under stressful conditions, as well as for industry and in cancer biology, where it is important to understand how the genotype relates to the phenotype.

show abstract

“…Spore formation in Saccharomyces cerevisiae is a complex and tightly regulated pathway involving the induction of a large number of genes. Hanlon et al [21] identified MRPS17 in the cDNA library of spore-specific genes. The homology search showed that the first third of Mrps17 has strong sequence similarity with the bacterial S17 protein, which indicates that Mrps17 is a mitochondrial ribosomal protein.…”

Section: Discussionmentioning

confidence: 99%

MRPS17 promotes invasion and metastasis through PI3K/AKT signal pathway and could be potential prognostic marker for gastric cancer

Zhou

Ouyang

et al. 2021

J. Cancer

View full text Add to dashboard Cite

In this study, the molecular mechanisms through which Mitochondrial Ribosomal Protein S17 (MRPS17) contributes to gastric cancer (GC) and its prognostic significance in GC have been explored. As a protein encoding gene, MRPS17 encodes a 28s proteins belonging the ribosomal protein S17P family. The specific roles and molecular mechanisms of MRPS17 in cancers remain ambiguous. It was revealed by analyzing data from TCGA and GEO that elevated expression of MRPS17 was significantly associated with invasion of GC and poor survival of GC patients. Then through univariate and multivariate Cox regression analyses it was demonstrated that MRPS17 an independent prognostic factor for GC patients (P<0.001). It was demonstrated by differentially expressed gene analysis and functional enrichment analysis that MPRS17 is related to PI3K/AKT pathway and Cell adhesion molecules (CAMs), while its function is mediated by collagen-containing extracellular matrix and receptor ligand/regulator activity. Then it was proven by in-vitro experiments that knocking down of MRPS17 gene in AGS and SGC7901 cells would significantly inhibit proliferation and invasion capability of these cells. Furthermore, it was revealed by cell immunofluorescence assay that as a ribosomalprotein, MRPS17 was mainly distributed in the cytoplasmic surface of cell membrane. Additionally, activation of PI3K/AKT pathway is responsible for malignant progression of glioma that was promoted by MRPS17. In conclusion, it was revealed in the present study that MRPS17 promoted invasion and metastasis of GC and potential molecular mechanisms through which it exerted its influences on GC were explored, suggesting its potential as a novel prognostic biomarker for GC.

show abstract

Analysis of the meiotic role of the mitochondrial ribosomal proteins Mrps17 and Mrpl37 in Saccharomyces cerevisiae

Cited by 7 publications

References 36 publications

The mitochondrial ribosomal protein of the large subunit, Afo1p, determines cellular longevity through mitochondrial back-signaling via TOR1

The mitochondrial ribosomal protein of the large subunit, Afo1p, determines cellular longevity through mitochondrial back-signaling via TOR1

Integrated Multi-Omics Analysis of Mechanisms Underlying Yeast Ethanol Tolerance

MRPS17 promotes invasion and metastasis through PI3K/AKT signal pathway and could be potential prognostic marker for gastric cancer

Contact Info

Product

Resources

About