Analysis of the Methanolic Extract of the Shoot of Plectranthus Hadiensis

Menon, Darsan B; Sasikumar, J. M.; Gopalakrishnan, V. K.

doi:10.7897/2230-8407.04549

Cited by 4 publications

(6 citation statements)

References 8 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Concerning its phytochemical analysis and biological activity, polyphenols, alkaloids, tannins, cardiac glycosides, saponins, and terpenoids have been identified in different parts, such as roots, stems, leaves, and whole extracts [17][18][19][20][21][22][23][24][25][26][27][28][29][30][31][32]. Although some of these compounds have been isolated, the phytochemistry of this genus is far from being fully known [31].…”

Section: Introductionmentioning

confidence: 99%

“…Other studies reported on its anti-inflammatory activity (inhibiting cyclooxygenases 1 and 2 (COX-1, COX-2) and lipoxygenases 2 and 15 (LOX-2, LOX-15) [17,31,36]. It also exerts antioxidant [20][21][22]24,32,36], larvicidal [18], insecticidal [23], and antiplasmodial activities [25,27], and is used to treat sore throat, fever, and nauseas [37].…”

Section: Introductionmentioning

confidence: 99%

“…Concerning its antitumoral properties, this plant has been described to be useful for the treatment of abdominal, brain, breast, intestinal, prostate, skin, throat, and uterine cancers as well as of leukemia [37]. Methanolic and ethanolic P. hadiensis shoot extracts revealed cytotoxicity towards brine shrimp [20,22], and stem extracts were tested against HeLa cells [26]. The terpenoid fraction (monoterpenes and sesquiterpenes) isolated from the shoot had antiproliferative activity against human colon cancer cells (HCT-15) but was only minimally cytotoxic towards noncancerous normal cells.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Phytochemical Study and Antiglioblastoma Activity Assessment of Plectranthus hadiensis (Forssk.) Schweinf. ex Sprenger var. hadiensis Stems

et al. 2022

View full text Add to dashboard Cite

Glioblastoma (GB) is the most malignant form of primary astrocytoma, accounting for more than 60% of all brain tumors in adults. Nowadays, due to the development of multidrug resistance causing relapses to the current treatments and the development of severe side effects resulting in reduced survival rates, new therapeutic approaches are needed. The genus Plectranthus belongs to the Lamiaceae family and is known to be rich in abietane-type diterpenes, which possess antitumor activity. Specifically, P. hadiensis (Forssk.) Schweinf. ex Sprenger has been documented for the use against brain tumors. Therefore, the aim of this work was to perform the bioguided isolation of compounds from the acetonic extract of P. hadiensis stems and to investigate the in vitro antiglioblastoma activity of the extract and its isolated constituents. After extraction, six fractions were obtained from the acetonic extract of P. hadiensis stems. In a preliminary biological screening, the fractions V and III showed the highest antioxidant and antimicrobial activities. None of the fractions were toxic in the Artemia salina assay. We obtained different abietane-type diterpenes such as 7α-acetoxy-6β-hydroxyroyleanone (Roy) and 6β,7β-dihydroxyroyleanone (DiRoy), which was also in agreement with the HPLC-DAD profile of the extract. Furthermore, the antiproliferative activity was assessed in a glioma tumor cell line panel by the Alamar blue assay. After 48 h treatment, Roy exerted strong antiproliferative/cytotoxic effects against tumor cells with low IC50 values among the different cell lines. Finally, we synthesized a new fluorescence derivative in this study to evaluate the biodistribution of Roy. The uptake of BODIPY-7α-acetoxy-6β-hydroxyroyleanone by GB cells was associated with increased intracellular fluorescence, supporting the antiproliferative effects of Roy. In conclusion, Roy is a promising natural compound that may serve as a lead compound for further derivatization to develop future therapeutic strategies against GB.

show abstract

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Phytochemical Study and Antiglioblastoma Activity Assessment of Plectranthus hadiensis (Forssk.) Schweinf. ex Sprenger var. hadiensis Stems

et al. 2022

View full text Add to dashboard Cite

show abstract

“…The top genera medicinal orchids are Dendrobium (23 species), Calanthe (7 species), Liparis (6 species), Bulbophyllum, Cymbidium, Eulophia, Habenaria, Phaius (each 4 species) and Coelogyne, Phalaenopsis, Vanilla (each with 3 species). Genera of Calanthe, Coelogyne, Cymbidium, and Dendrobium [3] and Vanilla are among the medicinal orchid [21].…”

Section: Resultsmentioning

confidence: 99%

Inventorying medicinal orchid in Indonesia from global database

Wati¹,

Astuti²,

Cahyaningsih³

2023

E3S Web of Conf.

View full text Add to dashboard Cite

The global database plays an important role in preserving vital data and information that aids in the conservation and sustainable use of organisms, including plant species. Many data related to Orchidaceae as one of the largest families of flowering plant species are stored in global databases. An inventory study was done on the medicinal orchid species distributed in Indonesia from several global databases. This study aimed to acquire data from several global databases (i.e., POWO, MPNS, and IUCN REDLIST) related to what and where species are distributed in Indonesia, which species have medicinal records, and other information supporting conservation and sustainable use. The result showed six thousand orchid species with 200 genera in Indonesia. Bulbophyllum, Dendrobium, and Crepidium are the top five biggest genera. More than five thousand orchid species are endemic and distributed mainly in Papua, Kalimantan, and Jawa. About 130 orchid species have medicinal use; 115 orchid species are documented as medicinal plants worldwide, including 39 species recorded in Indonesia. IUCN Redlist has assessed 430 species, with 87 species included in IUCN Redlist species; one medicinal species listed as endangered species (Vanilla planifolia Andrews); and five species assessed as least concerned species. These findings could be important as a foundation for future conservation and sustainable use studies, not only in Indonesia but also in the world.

show abstract

“…A leaf extract displayed low larvicidal activity against the fourth instar larvae of Aedes aegypti , a dengue fever vector (LC 50 : 489.278 µ g/mL) [ 112 ]. A terpene-rich methanolic extract of the shoot part and a methanolic extract of the stem was investigated for cytotoxicity using a shrimp brine lethality assay (LC 50 : 145 µ g/mL) and against HeLa cells (141.3 μ g/mL) [ 113 , 114 ]. Other studies include (a) antibacterial susceptibility single dose and antioxidant activity studies [ 115 – 117 ]; (b) two studies investigating the antioxidant, antiproliferative, and antiinflammatory properties of the shoot parts [ 118 , 119 ]; and (c) a study screening several Plectranthus species, including P. hadiensis , for antiinflammatory effects [ 120 ].…”

Section: Resultsmentioning

confidence: 99%

A Bibliographic Assessment Using the Degrees of Publication Method: Medicinal Plants from the Rural Greater Mpigi Region (Uganda)

Schultz

Anywar

Quave

et al. 2021

Evidence-Based Complementary and Alternative Medicine

View full text Add to dashboard Cite

In ethnopharmacological research, many field assessment tools exist. Yet, these miss that critical point of how to really determine which species merit the costly lab studies, e.g., evaluation of traditional use via pharmacological assays and isolation of bioactive secondary metabolites. This gap can be filled with the introduction of a new tool for literature assessment: the Degrees of Publication (DoPs). In this study, its application is illustrated through an extensive bibliographic assessment of 16 medicinal plant species that were recently identified in the Greater Mpigi region of Uganda as being frequently used by local traditional healers in the treatment of medical disorders (namely, Albizia coriaria, Cassine buchananii, Combretum molle, Erythrina abyssinica, Ficus saussureana, Harungana madagascariensis, Leucas calostachys, Microgramma lycopodioides, Morella kandtiana, Plectranthus hadiensis, Securidaca longipedunculata, Sesamum calycinum subsp. angustifolium, Solanum aculeastrum, Toddalia asiatica, Warburgia ugandensis, and Zanthoxylum chalybeum). These species are suspected to be understudied, and a thorough bibliographic assessment has not been previously performed. Thus, the objectives of our study were to undertake a comparative assessment of the degree to which each of these plant species has been studied in the past, including evaluation of the quality of the journals where results were published in. The determination of the DoPs enabled successful assessment of the degrees to which each individual plant species has been studied so far, while also taking into account the methodological “research chain of ethnopharmacology” from ethnobotanical studies (“traditional use”) to pharmacological assays (“bioactivity”) and finally to pharmacognostic research (“structure elucidation”). The significance of a research paper was assessed by determining whether its journal and publishing house were members of the Committee on Publication Ethics (COPE). In total, 634 peer-reviewed publications were reviewed covering the period of 1960–2019, 53.3% of which were published in journals and by publishing houses affiliated with COPE (338 publications). The literature assessment resulted in the identification of understudied plants among the selected species. The majority of plants reviewed have not been sufficiently studied; six species were classified as being highly understudied and three more as being understudied: C. buchananii, F. saussureana, L. calostachys, M. lycopodioides, M. kandtiana, and S. calycinum subsp. angustifolium and A. coriaria, P. hadiensis, and S. aculeastrum, respectively. The newly introduced DoPs are a useful tool for the selection of traditionally used species for future laboratory studies, especially for pharmacological bioassays, isolation procedures, and drug discovery strategies.

show abstract

Analysis of the Methanolic Extract of the Shoot of Plectranthus Hadiensis

Cited by 4 publications

References 8 publications

Phytochemical Study and Antiglioblastoma Activity Assessment of Plectranthus hadiensis (Forssk.) Schweinf. ex Sprenger var. hadiensis Stems

Phytochemical Study and Antiglioblastoma Activity Assessment of Plectranthus hadiensis (Forssk.) Schweinf. ex Sprenger var. hadiensis Stems

Inventorying medicinal orchid in Indonesia from global database

A Bibliographic Assessment Using the Degrees of Publication Method: Medicinal Plants from the Rural Greater Mpigi Region (Uganda)

Contact Info

Product

Resources

About