Analysis of the Microbiota on Lettuce (Lactuca sativa L.) Cultivated in South Korea to Identify Foodborne Pathogens

Yu, Yeon-Cheol; Yum, Su-Jin; Jeon, Da-young; Jeong, Hee Gon

doi:10.4014/jmb.1803.03007

Cited by 18 publications

(25 citation statements)

References 53 publications

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“…Proteobacteria , particularly Gammaproteobacteria , was the dominant phylum of isolated endophytes, consistent with the root and leaf microbial communities of a congeneric plant species, Lactuca sativa 39 , 40 . One species of the Gammaproteobacteria , K. cowanii , was detected in all L. serriola populations.…”

Section: Discussionsupporting

confidence: 57%

Invasive Lactuca serriola seeds contain endophytic bacteria that contribute to drought tolerance

Jeong

Kim

Choi

et al. 2021

Sci Rep

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The mutualistic relationship between alien plant species and microorganisms is proposed to facilitate or hinder invasive success, depending on whether plants can form novel associations with microorganisms in the introduced habitats. However, this hypothesis has not considered seed endophytes that would move together with plant propagules. Little information is available on the seed endophytic bacteria of invasive species and their effects on plant performance. We isolated the seed endophytic bacteria of a xerophytic invasive plant, Lactuca serriola, and examined their plant growth-promoting traits. In addition, we assessed whether these seed endophytes contributed to plant drought tolerance. Forty-two bacterial species were isolated from seeds, and all of them exhibited at least one plant growth-promoting trait. Kosakonia cowanii occurred in all four tested plant populations and produced a high concentration of exopolysaccharides in media with a highly negative water potential. Notably, applying K. cowanii GG1 to Arabidopsis thaliana stimulated plant growth under drought conditions. It also reduced soil water loss under drought conditions, suggesting bacterial production of exopolysaccharides might contribute to the maintenance of soil water content. These results imply that invasive plants can disperse along with beneficial bacterial symbionts, which potentially improve plant fitness and help to establish alien plant species.

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Section: Discussionsupporting

confidence: 57%

Invasive Lactuca serriola seeds contain endophytic bacteria that contribute to drought tolerance

Jeong

Kim

Choi

et al. 2021

Sci Rep

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“…Serratia and Pantoea are primary spoilage bacteria in meat and meat products during aerobic storage [ 65 , 66 ]. Therefore, Proteus can be used as a basis for biomarker research to find harmful bacteria [ 67 ]. P. unclassified was negatively correlated with 23 species and consistent with these observations.…”

Section: Discussionmentioning

confidence: 99%

Dynamic Changes of Bacterial Communities and Microbial Association Networks in Ready-to-Eat Chicken Meat during Storage

Qiu

Xiao

et al. 2022

Foods

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Ready-to-eat (RTE) chicken is a popular food in China, but its lack of food safety due to bacterial contamination remains a concern, and the dynamic changes of microbial association networks during storage are not fully understood. This study investigated the impact of storage time and temperature on bacterial compositions and microbial association networks in RTE chicken using 16S rDNA high-throughput sequencing. The results show that the predominant phyla present in all samples were Proteobacteria and Firmicutes, and the most abundant genera were Weissella, Pseudomonas and Proteus. Increased storage time and temperature decreased the richness and diversity of the microorganisms of the bacterial communities. Higher storage temperatures impacted the bacterial community composition more significantly. Microbial interaction analyses showed 22 positive and 6 negative interactions at 4 °C, 30 positive and 12 negative interactions at 8 °C and 44 positive and 45 negative interactions at 22 °C, indicating an increase in the complexity of interaction networks with an increase in the storage temperature. Enterobacter dominated the interactions during storage at 4 and 22 °C, and Pseudomonas did so at 22 °C. Moreover, interactions between pathogenic and/or spoilage bacteria, such as those between Pseudomonas fragi and Weissella viridescens, Enterobacter unclassified and Proteus unclassified, or those between Enterobacteriaceae unclassified and W.viridescens, were observed. This study provides insight into the process involved in RTE meat spoilage and can aid in improving the quality and safety of RTE meat products to reduce outbreaks of foodborne illness.

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“…All metropolitan regions had positive samples for Salmonella spp., revealing its wide geographic distribution. The presence of S. aureus in the studied samples suggests danger to the population, since Yu et al (2018) classifies it as one of the main pathogens causing food pathologies, with emphasis on intoxications. This microorganism proves to be an excellent indicator of manipulation of objects and food, because it is part of the microbiota of the skin and mucosa of the human being (Brooks et al, 2014).…”

Section: Microbiological and Parasitological Analysismentioning

confidence: 99%

Detection of enteropathogens and research of pesticide residues in Lactuca sativa from traditional and agroecological fairs

et al. 2022

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Agroecological production represents a new reality in vegetable farming. Thus, the study aimed to search for the presence of pesticides in agroecological samples and microbiological and parasitological contamination in Lactuca sativa L. of agroecological and conventional fairs in Alagoas. Thirty-two samples were collected, a hygienic-sanitary checklist was performed, along with coliform, Salmonella spp., Staphylococcus aureus research and Gas Chromatography with Mass Spectrometer (GC-MS) for pesticide research; using two methodologies for parasitological studies. Thirty-two samples were analyzed, with a sanitary adequacy level at 45% and 38% for fairs (agroecological and conventional) respectively, with three samples (9.4%) in total, being adequate to the microbiological pattern according to RDC no. 12/2001. Among the 23 samples which were tested positive for parasites, 69.5% originated from samples extracted at conventional fairs, and 53% of every positive samples presented Entamoeba coli as the main contaminant. All together, no traces of deltamethrin were detected, but in two of the samples a peak retention for diphenoconazole was detected. In conclusion, the fairs represent an adequate environment for human pathogens and indicators. Therefore greater hygiene training of vegetable traders and consumers in the state of Alagoas is required, especially in conventional fairs, which presented more critical microbiological and parasitological indexes comparing to fairs with agroecological products; however, the presence of difenoconazole in two samples of agricultural fairs suggests accidental contamination or indiscriminate use of this chemical in the production of Lactuca sativa, however, products with agroecological origin represents a better choice for the consumers.

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Analysis of the Microbiota on Lettuce (Lactuca sativa L.) Cultivated in South Korea to Identify Foodborne Pathogens

Cited by 18 publications

References 53 publications

Invasive Lactuca serriola seeds contain endophytic bacteria that contribute to drought tolerance

Invasive Lactuca serriola seeds contain endophytic bacteria that contribute to drought tolerance

Dynamic Changes of Bacterial Communities and Microbial Association Networks in Ready-to-Eat Chicken Meat during Storage

Detection of enteropathogens and research of pesticide residues in Lactuca sativa from traditional and agroecological fairs

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