Analysis of the Molecular Evolutionary History of the Ascorbate Peroxidase Gene Family: Inferences from the Rice Genome

Teixeira, Felipe Karam; Menezes-Benavente, Larissa; Margis, Rogério; Margis‐Pinheiro, Márcia

doi:10.1007/s00239-004-2666-z

Cited by 169 publications

(181 citation statements)

References 39 publications

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“…This enzyme catalyzes the reduction of H 2 O 2 using ascorbate as the specific electron donor (Asada, 1992;Teixeira et al, 2004). In our study, treatment with cement dust enhanced APX activity in Arabidopsis plants.…”

Section: Discussionsupporting

confidence: 51%

Effects of Cement Dust on the Physiological Activities of <i>Arabidopsis thaliana</i>

Abu-Romman¹,

Alzubi²

2015

American Journal of Agricultural and Biological Sciences

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Air pollution exerts detrimental effects on plant ecosystems and restricts agricultural productivity. Cement dust is considered to be one of the most harmful air pollutants of industrial origin and is a limiting factor for plant growth and productivity. This study aims to uncover the impacts of cement dust on photosynthetic pigments, protein content, hydrogen peroxide (H 2 O 2 ) accumulation, lipid per oxidation and antioxidant enzyme activities of Arabidopsis thaliana. In this study, Arabidopsis plants were dusted with cement at a rate of 1.5 g per 1-m 2 area and measurements were undertaken at 10 days after cement dust application. Treatment with cement dust resulted insignificant reductions in chlorophyll content and total soluble protein accumulation. Neither carotenoid nor starch content of Arabidopsis plants was affected by exposure to cement dust, whereas protease activity was significantly enhanced in cement dust-treated plants. Furthermore, exposure to cement dust significantly enhanced the production of H 2 O 2 , a product of oxidative stress, in the leaves of Arabidopsis plants. Moreover, Malondialdehyde (MDA) content, a product of lipid per oxidation, significantly increased after exposure to cement dust. In response to cement dust, activities of scavenging enzymes such as Ascorbate Peroxides (APX), Superoxide Dismutase (SOD) and Guaiacol Peroxidase (GPX) increased, whereas the activity of Catalase (CAT) activity decreased. The present results suggest that cement dust induced oxidative stress in Arabidopsis plants through the generation of Reactive Oxygen Species (ROS), induction of lipid peroxidation and up regulation of antioxidant enzyme activities.

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Section: Discussionsupporting

confidence: 51%

Effects of Cement Dust on the Physiological Activities of <i>Arabidopsis thaliana</i>

Abu-Romman¹,

Alzubi²

2015

American Journal of Agricultural and Biological Sciences

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“…5A), which is putatively localized in the thylakoid (Teixeira et al, 2004). Additionally, OsHTAS was simultaneously localized to the nucleus and cytoplasm (Fig.…”

Section: Discussion the Oshtas Gene Functions In Leaf Blade To Modulamentioning

confidence: 90%

The RING Finger Ubiquitin E3 Ligase OsHTAS Enhances Heat Tolerance by Promoting H2O2-Induced Stomatal Closure in Rice

et al. 2015

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Heat stress often results in the generation of reactive oxygen species, such as hydrogen peroxide, which plays a vital role as a secondary messenger in the process of abscisic acid (ABA)-mediated stomatal closure. Here, we characterized the rice (Oryza sativa) HEAT TOLERANCE AT SEEDLING STAGE (OsHTAS) gene, which plays a positive role in heat tolerance at the seedling stage. OsHTAS encodes a ubiquitin ligase localized to the nucleus and cytoplasm. OsHTAS expression was detected in all tissues surveyed and peaked in leaf blade, in which the expression was concentrated in mesophyll cells. OsHTAS was responsive to multiple stresses and was strongly induced by exogenous ABA. In yeast two-hybrid assays, OsHTAS interacted with components of the ubiquitin/26S proteasome system and an isoform of rice ascorbate peroxidase. OsHTAS modulated hydrogen peroxide accumulation in shoots, altered the stomatal aperture status of rice leaves, and promoted ABA biosynthesis. The results suggested that the RING finger ubiquitin E3 ligase OsHTAS functions in leaf blade to enhance heat tolerance through modulation of hydrogen peroxide-induced stomatal closure and is involved in both ABA-dependent and DROUGHT AND SALT TOLERANCE-mediated pathways.

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“…Recent genome-wide analysis in higher plants indicated that APX belongs to a multigenic family, since there are nine genes in Arabidopsis, eight in rice, and seven in tomato. [43][44][45] These APX families are further classified into three subfamilies according to subcellular localization, viz., chloroplastic, cytosolic, and microbody isoenzymes. 5) Chloroplastic isoenzymes have stroma-soluble (sAPX) and thylakoid membranebound (tAPX) forms.…”

Section: Enzymatic and Molecular Properties Of Apx Isoenzymesmentioning

confidence: 99%

Recent Advances in Ascorbate Biosynthesis and the Physiological Significance of Ascorbate Peroxidase in Photosynthesizing Organisms

Ishikawa

Shigeoka

2008

Bioscience, Biotechnology, and Biochemistry

250

168

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Ascorbate (AsA), the most abundant water-soluble redox compound in plants and eukaryotic algae, has multiple functions. There is compelling genetic evidence that the biosynthesis of AsA proceeds via a D-mannose/ L-galactose pathway and is the most significant source of AsA in plants. AsA plays important roles in antioxidative defense, particularly via the AsA/glutathione cycle. AsA peroxidase (APX) plays a central role in the cycle and is emerging as a key enzyme in cellular H 2 O 2 metabolism. Plants possess diverse APX isoenzymes in cellular compartments, including the chloroplast, cytosol, and microbody. In algae, however, the number and distribution of APX proteins are quite limited. Recent progress in molecular biological analysis of APX isoenzymes has revealed elaborate mechanisms for the tissue-dependent regulation of two chloroplastic APX isoenzymes by alternative splicing, and for redox regulation of cytosolic APX gene expression in response to light stress. Furthermore, transgenic plants overexpressing a chloroplastic APX isoenzyme enable us to evaluate the behavior of the enzyme under conditions of photo-oxidative stress. Molecular physiological analysis has revealed that cytosolic APX is part of the system modulating the cellular H 2 O 2 level in redox signaling.

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Analysis of the Molecular Evolutionary History of the Ascorbate Peroxidase Gene Family: Inferences from the Rice Genome

Cited by 169 publications

References 39 publications

Effects of Cement Dust on the Physiological Activities of <i>Arabidopsis thaliana</i>

Effects of Cement Dust on the Physiological Activities of <i>Arabidopsis thaliana</i>

The RING Finger Ubiquitin E3 Ligase OsHTAS Enhances Heat Tolerance by Promoting H2O2-Induced Stomatal Closure in Rice

Recent Advances in Ascorbate Biosynthesis and the Physiological Significance of Ascorbate Peroxidase in Photosynthesizing Organisms

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