Analysis of the nondialyzable constituents in a sample of succus liquiritiae

Abstract

The nondialyzable components in a sample of succus liquirifiur have been separated into four fractions by ammonium sulphate fractionation and precipitation with organic solvents. The chemical composition and some properties of these fractions are given.Succus fiquirifiae contains a hemicellulose fraction, starch, and a fair amount of various brown peptide-carbohydrate compounds (glycoproteins).The inhalant allergen in succ. /iq. is associated with one of the glycoprotein fractions. The relation of the allergenicity of succ. liq. to browning reactions is discussed.

The Chemical Classification of Atopic Allergens: An Attempt at Integration

“…liquorice [8], tomato [21], ipecac [7], cow's milk [22] and egg-white [23]. A brief scrutiny of the analytical composition of these allergens at once exposes the irrelation between whole allergen molecules from different allergenic materials.…”

Kapok Allergens

“…The failure to stain with protein dyes has been interpreted be fore (8,19) as indicative of an almost complete blockade of free protein amino groups, in accordance with the evidence for N-glycosidic linkages; the negative results with the carbohydrate stain are consistent with a branched hetcropolysaccharide structure of the glycan moiety, as in the case of allergens from liquorice (19). The successful uptake of protein dye after pretreatment with NaOIi favours an explanation in terms of the unmasking of protein amino groups by the mechanism of /^-elimination induced by rearrange ments in the substituent sugar residues under alkaline conditions (16).…”

Allergens

“…A comprehensive review of the chemistry of allergens is beyond the scope of this paper both because of space limitations and because recent publication of a monumental, critical review containing 558 references would make such an attempt redundant from a literature reference standpoint. Berrens (1971), in a monograph of 298 pages titled "The Chemistry of Atopic Allergens," has reviewed the subject of allergens in eight categories, namely the pollens, including timothy and cocksfoot (Augustin and Hayward, 1962), ryegrass (Johnson and Marsh, 1966a), alder (Herbertson et al, 1958), ragweed, the greatest single cause of allergy from a natural source in the U. S. (Goldfarb, 1968;King et al, 1967;Richter and Sehon, 1960;Robbins et al, 1966;Underdown and Goodfriend, 1969); allergens from vegetable dusts and fibers, including ipecac (Berrens and Young, 1963), liquorice (Berrens, 1964), pyrethrum (Zucker, 1965), kapok (Berrens, 1966a; Coulson et al, 1944), and cotton linters (Berrens and Versie, 1967;Coulson and Stevens, 1940); allergens from seeds, mainly cottonseed (Spies et al, 1940b(Spies et al, , 1960 and castor beans (Panzani and Layton, Spies and Coulson, 1964); allergens from epithelial tissues such as horse dandruff (Stanworth, 1957), human dandruff 1968a), and insects, exclusive of biting and stinging varieties (Shulman, 1968); molds (Barker et al, 1967;Prince et al, 1961); the ubiquitous house dust allergens (Berrens, 1970); allergens from foods (Bleumink, 1970), including tomato (Bleumink et al, 1967), cow's milk (Bleumink and Young, 1968;Goldstein and Heiner, 1970;Hanson and Johansson, 1970;Spies, 1973), egg (Ble...…”