Analysis of the ovine respiratory syncytial virus (RSV) G glycoprotein gene defines a subgroup of ungulate RSV

Abstract

Respiratory syncytial virus (RSV) has been isolated from sheep suffering from respiratory tract disease. Since the greatest differences between bovine RSV and human RSV are found on the attachment G protein, we have determined the nucleotide and deduced amino acid sequences of the G gene of ovine RSV. The latter contained 838 nucleotides and had a major open reading frame encoding a protein of 263 residues, and shared 73 % nucleotide sequence identity with that of bovine RSV. The deduced amino acid sequence of the ovine RSV G protein showed only 60 % amino acid identity with the G protein of bovine RSV. Despite the low level of identity, there were similarities in the predicted hydropathy profiles of the G proteins of ovine and bovine RSV. The intergenic sequences for the SH-G and G-F gene junctions of ovine RSV showed 64 and 57% identity respectively with the corresponding regions of the bovine RSV. Our results indicate that ovine and bovine RSV might be classified as two subgroups of an ungulate RSV.

Analysis of Ruminant Respiratory Syncytial Virus Isolates by RNAse Protection of the G Glycoprotein Transcripts

“…10 Polyclonal antibodies to bovine RSV immunoprecipitated the N, P, M, F, G, 22K, 1C, and 1B proteins of ovine RSV strains WSU 83-1578 and WSU 87-6750. 21 The SH protein is a surface viral antigen that is present in 4 different glycosylated forms and might provoke a heterogeneous and divergent immune response between different subgroups. Studies on bovine and ovine SH protein using RPA and monoclonal antibodies are needed to further examine the genetic and antigenic variations between the 2 ruminant RSV subgroups.…”

Analysis of bovine respiratory syncytial virus envelope glycoproteins in cell fusion.

“…BRSV G and SH proteins are highly variable compared to the G and SH proteins of ORSV (60 % and 69 % amino acid identity, respectively ; Mallipeddi & Samal, 1993 ;Alansari & Potgieter, 1994 b) and HRSV (30 % and 38 % amino acid identity respectively ; Lerch et al, 1990 ;Samal & Zamora, 1991). In order to determine whether typespecific interactions are required to elicit cell fusion, a combination of BRSV envelope proteins with ORSV or HRSV envelope proteins was coexpressed (Fig.…”

The Ovine Respiratory Syncytial Virus F Gene Sequence and its Diagnostic Application

“…The F25 primer used for first strand cDNA synthesis (5Ј-TGATCTCATATTTGCAGGG-3Ј) represents the complementary sequence of bases 43-25 of the published sequence of M2 gene of ovine RSV. 4 The second primer, F19 (5Ј-GAGACAAGGGTGTATATAG-3Ј), representing part of the published intergenic sequence between F and G genes of ovine RSV, 19 was used with the F25 primer for amplification.…”