The indoor environment in non-university classrooms is one of the most analyzed problems in the thermal comfort and indoor air quality (IAQ) areas. Traditional schools in southern Europe are usually equipped with heating-only systems and naturally ventilated, but climate change processes are both progressively increasing average temperatures and lengthening the warm periods. In addition, air renewal is relayed in these buildings to uncontrolled infiltration and windows' operation, but urban environmental pollution is exacerbating allergies and respiratory conditions among the youth population. In this way, this exposure has a significant effect on both the academic performance and the general health of the users. Thus, the analysis of the occupants' noticed symptoms and their perception of the indoor environment is identified as a potential complementary tool to a more comprehensive indoor comfort assessment. The research presents an analysis based on environmental sensation votes, perception, and indoor-related symptoms described by students during lessons contrasted with physical and measured parameters and operational scenarios. This methodology is applied to 47 case studies in naturally ventilated classrooms in southern Europe. The main conclusions are related to the direct influence of windows' operation on symptoms like tiredness, as well as the low impact of CO 2 concentration variance on symptomatology because they usually exceeded recommended levels. In addition, this work found a relationship between symptoms under study with temperature values and the environmental perception votes, and the special impact of the lack of suitable ventilation and air purifier systems together with the inadequacy of current thermal systems.Atmosphere 2020, 11, 357 2 of 27 windows' operation, much more than usually found in central and northern Europe. This develops a behavior that could be defined as hybrid or mixed mode, with thermal systems operated and with a significant part of the time with the windows open. In addition, climate change processes are progressively lengthening the warm periods with greater presence within the school season. In addition, urban environmental pollution and pollen are exacerbating allergies and respiratory conditions among the youth population [19,20], especially in the case of outdoor atmospheric particulate matter (PM) with a diameter of less than 2.5 micrometers (PM 2.5) [12,21,22]. This context generates a situation of specificity where further study is necessary, given the different exposure scenarios with a greater influx from the outside although varying over time.Given that ventilation is one of the main variables which affects the degree of environmental comfort [23,24], the European ventilation standard EN 13779:2008 [25], through its Spanish transposition [26], establishes a minimum outdoor airflow to guarantee the adequate indoor air quality (IAQ) in non-residential buildings. Mainly, its focus is to control CO 2 concentration, pollutants, and suspended particles [27] to avoid ...