Analysis of the polyphenolic fraction of propolis from different sources by liquid chromatography–tandem mass spectrometry

Gardana, Claudio; Scaglianti, Martina; Pietta, Piergiorgio; Simonetti, Paolo

doi:10.1016/j.jpba.2007.06.022

Cited by 223 publications

(182 citation statements)

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“…The content of phenolic antioxidants showed a good correlation with the characteristic antioxidant activities (r 2 = 0.96 for propolis and 0.90 for honey) (Buratti et al 2007). Flavonoids pinobanksin, pinocembrin, quercetin, chrysin, galangin, luteolin and kaempferol were reported to be present in honey , while pinocembrin, pinobanksin and chrysin are characteristic flavonoids of propolis (Gardana et al 2007); these flavonoids were determined in most of the previously analysed European honey samples (Yao et al 2004). The screening of honey phenolic extracts by HPLC resulted in the identification of p-coumaric acid, chrysin, kaempferol, and apigenin in all samples tested.…”

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confidence: 85%

Contents of major phenolic and flavonoid antioxidants in selected Czech honey

Lachman¹,

Hejtmánková²,

Sykora³

et al. 2010

Czech J. Food Sci.

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Lachman J., Hejtmánková A., Sýkora J., Karban J., Orsák M., Rygerová B. (2010): Contents of major phenolic and flavonoid antioxidants in selected Czech honey. Czech J. Food Sci., 28: 412-426.The chemical constitution of antioxidants contained in honey is derived from its origin. Forty honey samples (harvest 2006), which came from various locations of the Czech Republic and varied in their origins, were evaluated spectrophotometrically for their total polyphenol content, total flavonoids and 3',4'-dihydroxyflavones and flavonols, and major antioxidants were identified by HPLC-DAD and GC-MS. The kind of honey, location, and date of the honey harvest were shown to have a significant effect on the contents of phenolic antioxidants (average content 11.02 mg gallic acid equivalents/100 g), total flavonoids (0.66 mg quercetin equivalents/100 g), and 3',4'-dihydroxyflavones and flavonols (4.32 µg quercetin equivalents/100 g). In the Czech honey, ferulic acid (0.11 mg/100 g) and chrysin (0.06 mg/100 g) and other minority phenolics and flavonoids were identified and quantified as honey phenolic antioxidants contained. The results obtained support and extend complete knowledge on the contents of bioactive phenolics in the Czech honey, which could serve as a good source of natural antioxidants effective in reducing the risk of the occurrence of heart disease, cancer, cataracts, different inflammatory processes and immuno-system decline.

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confidence: 85%

Contents of major phenolic and flavonoid antioxidants in selected Czech honey

Lachman¹,

Hejtmánková²,

Sykora³

et al. 2010

Czech J. Food Sci.

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“…The phenolic profile of the propolis extracts was analyzed by reversed-phase HPLC, based on the method of Gardana et al [23] with some modifications, as described below. The HPLC analysis was performed on a Knauer Smartline separation module equipped with a Knauer smartline autosampler 3800, a cooling system set to 4°C, and a Knauer UV detector 2500.…”

Section: Hplc Analysismentioning

confidence: 99%

“…A large number of analytical methods have been used for the analysis of phenolic compounds in propolis, including spectrophotometry [17], thin-layer chromatography [18], gas chromatography-mass spectrometry (GC-MS) [19], highperformance liquid chromatography (HPLC) [20], liquid chromatography-mass spectrometry (LC-MS) [21][22][23], electrospray ionization-mass spectrometry (ESI-MS) and electrospray ionization-tandem mass spectrometry (ESI-MS n ) [24], capillary electrophoresis [25], and nuclear magnetic resonance (NMR) [26]. Among these methods, MS with ESI is one of the most-used techniques in the research of natural products, as it is able to analyze complex mixtures because of its high selectivity.…”

Section: Introductionmentioning

confidence: 99%

“…The chemical composition of European propolis was determined by GC-MS [11,19], LC-MS, and ESI-MS [21][22][23][24]. Also Moreira et al [27] have recently described the phenolic content and the antioxidant potential of Portuguese propolis from two different regions, including the Northeast, but still, their phenolic components were not elucidated.…”

Section: Introductionmentioning

confidence: 99%

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Phenolic characterization of Northeast Portuguese propolis: usual and unusual compounds

et al. 2009

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In this study, an ethanolic extract from Portuguese propolis was prepared, fractionated by highperformance liquid chromatography, and the identification of the phenolic compounds was done by electrospray mass spectrometry in the negative mode. This technical approach allowed the identification of 37 phenolic compounds, which included not only the typical phenolic acids and flavonoids found in propolis from temperate zones but also several compounds in which its occurrence have never been referred to in the literature. Four of the novel phenolic compounds were methylated and/or esterified or hydroxylated derivatives of common poplar flavonoids, although six peculiar derivatives of pinocembrin/pinobanksin, containing a phenylpropanoic acid derivative moiety in their structure, were also identified. Furthermore, the Portuguese propolis sample was shown to contain a p-coumaric ester derivative dimer.

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“…Não foram encontrados compostos fenólicos comuns de plantas terrestres como ácidos fenólicos e flavonoides (Robbins, 2003;Gardana et al, 2007;Lin & Harnly, 2007 Por outro lado, o pico 3 (derivado da micosporina palitinol) apresentou uma clara resposta à luz (Fig. 9).…”

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Análise de pigmentos fotossintetizantes e substâncias fenólicas em Gracilariopsis tenuifrons (C. J. Bird & E. C. Oliveira) Fredericq & Hommersand em diferentes intensidades de luz

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Analysis of the polyphenolic fraction of propolis from different sources by liquid chromatography–tandem mass spectrometry

Cited by 223 publications

References 20 publications

Contents of major phenolic and flavonoid antioxidants in selected Czech honey

Contents of major phenolic and flavonoid antioxidants in selected Czech honey

Phenolic characterization of Northeast Portuguese propolis: usual and unusual compounds

Análise de pigmentos fotossintetizantes e substâncias fenólicas em Gracilariopsis tenuifrons (C. J. Bird & E. C. Oliveira) Fredericq & Hommersand em diferentes intensidades de luz

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