Analysis of the presence of cutaneous and mucosal papillomavirus types in ductal lavage fluid, milk and colostrum to evaluate its role in breast carcinogenesis

Cazzaniga, Massimiliano; Gheit, Tarik; Casadio, Chiara; Khan, Noureen; Macis, Debora; Valenti, Francesco; Miller, Mara Jo; Sylla, Bakary S.; Akiba, Suminori; Bonanni, Bernardo; DeCensi, Andrea; Veronesi, Umberto; Tommasino, Massimo

doi:10.1007/s10549-008-0040-3

Cited by 33 publications

(23 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, contrary to a previous report (Weissenborn et al, 2005), the viral loads were not higher in AK compared with the other lesion types investigated. The viral loads in the present study might be lower due to the tape stripping method used, which reduces superficial layers harbouring HPV DNA from the lesions (Cazzaniga et al, 2008;Forslund et al, 2004). Three (15.8 %) of the HPV-positive patients harboured multiple HPV infections with any of the four novel types.…”

Section: Discussionmentioning

confidence: 64%

Four novel human betapapillomaviruses of species 2 preferentially found in actinic keratosis

Vasiljević

Hazard

Dillner

et al. 2008

Journal of General Virology

View full text Add to dashboard Cite

Recent studies have suggested an association between human papillomaviruses (HPVs), particularly species 2 members of the genus Betapapillomavirus, and squamous cell carcinoma (SCC) of the skin. As most of these viruses are uncharacterized, molecular characterization and epidemiology are needed to advance our understanding of their significance in carcinogenesis. This study determined the complete genomes of four betapapillomaviruses of species 2 from skin lesions designated , an isolate of an unpublished HPV type, and analysed their prevalence and viral loads in biopsies from SCC, actinic keratosis (AK), basal cell carcinoma, seborrhoeic keratosis and the healthy skin of 263 immunocompetent patients by HPV type-specific real-time PCR assays. Seventeen patients (6.5 %) harboured at least one of the four HPV types in their lesion, whereas seven patients (2.7 %) harboured one or more of the HPV types in healthy skin. Overall, the four viruses were more common in AK than in healthy skin (odds ratio 5.0, 95 % confidence interval 1.4-17.5), but the prevalence and viral loads were low. This characterization of expands the heterogeneity of members of species 2 of the genus Betapapillomavirus. However, as these types were found in only a few samples and in low amounts, a possible role in carcinogenesis remains elusive.

show abstract

Section: Discussionmentioning

confidence: 64%

Four novel human betapapillomaviruses of species 2 preferentially found in actinic keratosis

Vasiljević

Hazard

Dillner

et al. 2008

Journal of General Virology

View full text Add to dashboard Cite

show abstract

“…In addition, we detected cutaneous HPV types (HPV-15, -23, and -124). The presence of cutaneous HPV types was reported in the previous studies among women with breast cancer or at increased risk for breast cancer [10, 39]. The only isolate that screened by the FAB primer set has no significant similarity to any known HPV types.…”

Section: Discussionmentioning

confidence: 82%

Human Papillomavirus Is Associated with Breast Cancer in the North Part of Iran

Sigaroodi

Nadji

Naghshvar

et al. 2012

The Scientific World Journal

View full text Add to dashboard Cite

We have analyzed the possible relevance of HPV infection for breast cancer risk among Iranian women from north part of Iran. Among women with breast cancer, 25.9% had positive test results for HPV DNA in breast tumor samples in contrast to 2.4% of women with noncancer status (P=0.002). The infection of HPV has increased the risk of breast cancer (OR 14.247; 95% CI 1.558–130.284,P=0.019). The high-risk HPV genotypes (types 16 and 18) in samples of breast cancer patients were the predominant types (53.34%). Other genotypes detected in breast cancer were HPV-6, HPV-11, HPV-15, HPV-23, and HPV-124, and one isolate could not be genotyped compared to HPV reference sequences. While the sole detected HPV in control specimens was HPV-124. Our study reveals that HPV infection and age are the risk factors in breast cancer development in the north part of Iran.

show abstract

“…The assays were combined in a single chip in order to detect 19 mucosal high-risk and potential high-risk HPV types (16, 18, 26, 31, 33, 35, 39, 45, 51, 52, 53, 56, 58, 59, 66, 68, 70, 73 and 82) and 25 cutaneous HPV types from genus β (5, 8, 9, 12, 14, 15, 17, 19, 20, 21, 22, 23, 24, 25, 36, 37, 47, 49, 75, 76, 80, 92, 93 and 96). The assay for the detection of mucosal HPV type was slightly modified with respect to the previous version [11], as we have replaced the PCR forward primer for HPV-16 E7 [12]. In addition new oligonucleotides have been added to the chip in order to detect 15 α-HPV low-risk (6, 11, 34, 40, 42, 43, 44, 54, 55, 57, 61, 67, 71, 72, 74, 81, 83 and 84) and 6 γ-HPV types (4, 48, 50, 60, 65 and 95).…”

Section: Methodsmentioning

confidence: 99%

Clinicopathological Study of 13 Cases of Squamous Cell Carcinoma Complicating Hidradenitis Suppurativa

et al. 2009

View full text Add to dashboard Cite

Background: To the best of our knowledge, only 52 cases of squamous cell carcinoma (SCC) complicating hidradenitis suppurativa (HS) have been reported since 1958. We describe 13 new cases. Methods: We propose a clinical and histological analysis of our cases. We include these results in a review of previously reported cases to analyze a total of 65 patients. In our series of 13 cases, we also investigate the presence of human papillomavirus (HPV) in tumor samples, by polymerase chain reaction (PCR) on paraffin-embedded material. Results: Malignant transformation affects mainly men with a long-term history of genitoanal HS. Although our cases were 7 well-differentiated carcinomas and 6 verrucous carcinomas, lymphatic and visceral metastasis occurred in 2 and 3 cases, respectively. With PCR, we demonstrated presence of HPV in genitoanal tumoral lesions, principally HPV-16. Conclusion: SCC complicating HS evolves poorly, despite a good histological prognosis. Our results sustain the implication of HPV in the malignant transformation of HS.

show abstract

Analysis of the presence of cutaneous and mucosal papillomavirus types in ductal lavage fluid, milk and colostrum to evaluate its role in breast carcinogenesis

Cited by 33 publications

References 26 publications

Four novel human betapapillomaviruses of species 2 preferentially found in actinic keratosis

Four novel human betapapillomaviruses of species 2 preferentially found in actinic keratosis

Human Papillomavirus Is Associated with Breast Cancer in the North Part of Iran

Clinicopathological Study of 13 Cases of Squamous Cell Carcinoma Complicating Hidradenitis Suppurativa

Contact Info

Product

Resources

About