Analysis of the Protective Capacity of Three<i>Streptococcus suis</i>Proteins Induced under Divalent-Cation-Limited Conditions

Aranda, Jesús; Garrido, M. Elena; Cortés, Pilar; Llagostera, Montserrat; Barbé, Jordi

doi:10.1128/iai.00987-07

Cited by 41 publications

(50 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Interestingly, both cation-binding lipoproteins encoded by these operons (SsuiDRAFT 1237 and SsuiDRAFT 0103) are immunogenic when analysed on Western blots using immunologically reactive serum obtained from mice surviving after inoculation with the wild-type S. suis 89/1591 strain (Aranda et al, 2008). Furthermore, a high level of protection against S. suis challenge is obtained in mice when vaccinated with the product of the ssuiDRAFT 0103 ORF (Aranda et al, 2008). Different strategies have been developed to produce vaccines against pathogenic bacteria, including overexpression on the bacterial cell surface of divalent cation-uptake transporters which are induced in the presence of chelating agents, or by the construction of strains deficient in cationuptake repressors (Garrido et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

“…It has been shown that in S. suis, AdcR controls at least two operons related to Zn 2+ /Mn 2+ uptake, encoding a complete ABC transporter (SsuiDRAFT 1237-1240) and a divalent cation-binding lipoprotein (SsuiDRAFT 0103) (Aranda et al, 2008). Interestingly, both cation-binding lipoproteins encoded by these operons (SsuiDRAFT 1237 and SsuiDRAFT 0103) are immunogenic when analysed on Western blots using immunologically reactive serum obtained from mice surviving after inoculation with the wild-type S. suis 89/1591 strain (Aranda et al, 2008). Furthermore, a high level of protection against S. suis challenge is obtained in mice when vaccinated with the product of the ssuiDRAFT 0103 ORF (Aranda et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

“…In addition, iron can be particularly toxic under aerobic conditions, interacting with either oxygen or oxygen-reduced species (Touati, 2000). Consequently, micro-organisms require homeostatic mechanisms to control intracellular metal levels.Fur (ferric uptake regulator) is a transcriptional regulator of genes involved in iron uptake and storage in many Different strategies have been developed to produce vaccines against pathogenic bacteria, including overexpression on the bacterial cell surface of divalent cation-uptake transporters which are induced in the presence of chelating agents, or by the construction of strains deficient in cationuptake repressors (Garrido et al, 2008). In this context, the aim of this work has been to obtain knockout S. suis mutants for the regulator genes of either zinc/manganeseor iron-uptake systems to study their possible use as a protective tool against this pathogen.…”

mentioning

confidence: 99%

“…The survival of the mice was subsequently monitored for 7 days. Serum for Western blot assays was obtained as previously reported (Aranda et al, 2008).…”

mentioning

confidence: 99%

“…DIG-DNA-labelled protein complexes were detected according to the manufacturer's protocol (Roche). AdcR protein was purified as previously described (Aranda et al, 2008).…”

mentioning

confidence: 99%

See 4 more Smart Citations

Protective capacities of cell surface-associated proteins of Streptococcus suis mutants deficient in divalent cation-uptake regulators

et al. 2009

Self Cite

View full text Add to dashboard Cite

Many cell surface-associated, divalent cation-regulated proteins are immunogenic, and some of them confer protection against the bacterial species from which they are derived. In this work, two Streptococcus suis divalent cation uptake regulator genes controlling zinc/manganese and iron uptake (adcR and fur, respectively) were inactivated in order to study the protective capacities of their cell surface-associated proteins. The results obtained showed overexpression of a set of immunogenic proteins (including members of the pneumococcal histidine triad family previously reported to confer protection against streptococcal pathogens) in S. suis adcR mutant cell surface extracts. Likewise, genes encoding zinc transporters, putative virulence factors and a ribosomal protein paralogue related to zinc starvation appeared to be derepressed in this mutant strain. Moreover, protection assays in mice showed that although neither adcR-nor fur-regulated cell surface-associated proteins were sufficient to confer protection in mice, the combination of both adcR-and fur-regulated cell surface-associated proteins is able to confer significant protection (50 %, P50.038) against a challenge to mice vaccinated with them. INTRODUCTIONStreptococcus suis is an important pathogen that causes significant economic losses in the swine industry worldwide (Higgins et al., 1995). Infection caused by S. suis may vary from asymptomatic bacteraemia to fulminant systemic disease, with meningitis as the most outstanding feature (Higgins et al., 1995). It is also an important zoonotic agent for humans, who may acquire the infection through skin wounds upon contact with pigs and/or their by-products. Recently, during an outbreak in China, 215 human cases of S. suis infection were documented, and 39 of those patients died (Yu et al., 2006). More than 30 serotypes of S. suis, based on its capsular antigens, are currently known (Higgins et al., 1995). Serotype 2 is the most frequently isolated from diseased animals (Staats et al., 1997). Efforts to control infection are hampered by the lack of effective vaccines against S. suis. Furthermore, mechanisms involved in pathogenesis of this micro-organism are not yet completely understood (Gottschalk & Segura, 2000).Iron and zinc are essential components of many bacterial proteins either as structural or catalytic cofactors (Andrews et al., 2003;Babich & Stotzky, 1978). The concentration of both elements in the free state in the body fluids of mammals is extremely low in order to prevent bacterial proliferation. On the other hand, metals at high concentrations are toxic to micro-organisms. Toxicity occurs through the displacement of essential metals from their native binding sites or through ligand interactions (Babich & Stotzky, 1978; Chamnongpol et al., 2002). In addition, iron can be particularly toxic under aerobic conditions, interacting with either oxygen or oxygen-reduced species (Touati, 2000). Consequently, micro-organisms require homeostatic mechanisms to control intracellular metal levels.Fur (...

show abstract

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

mentioning

confidence: 99%

“…The survival of the mice was subsequently monitored for 7 days. Serum for Western blot assays was obtained as previously reported (Aranda et al, 2008).…”

mentioning

confidence: 99%

“…DIG-DNA-labelled protein complexes were detected according to the manufacturer's protocol (Roche). AdcR protein was purified as previously described (Aranda et al, 2008).…”

mentioning

confidence: 99%

See 3 more Smart Citations