Analysis of the relationship between Wx gene polymorphisms and amylose content in hulless barley

Abstract

Wu K., Yao X., Yao Y., Chi D., Feng Z. (2017): Analysis of the relationship between Wx gene polymorphisms and amylose content in hulless barley. Czech J. Genet. Plant Breed., 53: 144−152.I 2 -KI staining was used to phenotype 151 hull-less barley plants, which determined that five belonged to the waxy phenotype, namely 14-Z152, IG107028, Puebla, Hu Zhu Shuang Cao Ren, and APM-HC1905. Using the dual-wavelength method, the average amylose content of the 151 samples was 25.9%, ranging from 4.9 to 38.5%. The average amylose content of the five waxy varieties was 14.3%, ranging from 4.9 to 18.6%. Genomic DNA from 48 samples showing a significantly variable amylose content was used as template and PCR amplified using the primer pair P4. Statistical analysis indicated that the PCR product size positively correlated with amylose content. The Wx gene locus was determined to be polymorphic, and was positively correlated with amylose. Based on the electrophoresis results, the 48 samples were divided into 4 types. PCR product types I, II, III, and IV were 457, 481, 489, and 491 bp in length, respectively, with the respective amylose content ranges of 4.9-27%, 29-30%, 31-35%, and 36-38%. The primer P4 can be used as a complementary marker for the selection of hullles barley germaplasm with different amylose content.

Identification microRNAs and target genes in Tibetan hulless barley to BLS infection

“…This plant is widely cultivated on the Qinghai-Tibet Plateau and has served as a staple food for the Tibetan people since the 5th century CE (Zeng et al, 2018). The grains of Tibetan hulless barley not only contain abundant nutritional components and physiologically active compounds but are also rich in edible pigments necessary for the food manufacturing industry (Li et al, 2011;Wu et al, 2017). Barley leaf stripe (BLS), caused by Pyrenophora graminea, is a serious fungal disease affecting the yield and quality of Tibetan hulless barley on the Qinghai-Tibet Plateau (Si et al, 2019).…”

Identification and Profiling of microRNAs and Their Target Genes in Tibetan Hulless Barley in Response to Barley Leaf Stripe (BLS) Infection

“…This plant is widely cultivated on the Qinghai-Tibet Plateau and has served as a staple food for the Tibetan people since the 5th century CE [1,2]. Grains of Tibetan hulless barley not only contain abundant nutritional components and physiologically-active compounds, but also are rich in edible pigments necessary the food manufacturing industry [3,4].…”

Identification of molecular markers for starch content in barley (Hordeum vulgare L.) by genome-wide association studies based on bulked samples

“…Starch is the main storage component in barley grain (Wu et al ., 2017), and it ranges from 38.7 to 59.3% in the barley core collection of the USDA National Small Grain Collection (Bowman et al ., 2001). The content and properties of starch affect the quality of barley products (Zhu, 2017).…”