Analysis of the Relationship of Itgb3, Fv, and Fii Gene Polymorphism With the Development of Diabetic Foot Syndrome

Abstract

Introduction. There is a steady increase in the incidence of type 2 diabetes worldwide. One of the most formidable complications of this pathology is the development of diabetic foot syndrome, which is accompanied by up to 50% of cases of high amputations, disability, increased mortality and a decrease in the quality of life of patients. The aim of the study: to study the prevalence of genotype and allele variants of the T1565C polymorphism of the ITGB3 gene, the G1691A polymorphism of the FV gene, and the G20210A polymorphism of the FII gene in patients with the development of diabetic foot syndrome, as well as in the control group living in the Trans-Baikal Territory. Material and methods. Polymorphism T1565C of the ITGB3 gene, G1691A polymorphism of the FV gene, and G20210A polymorphism of the FII gene were studied by PCR methods in 100 apparently healthy individuals and 198 patients with a mixed form of diabetic foot syndrome. The statistical significance of differences was assessed using Pearson’s chi-square test. Results. The T/T genotype of T1565C polymorphism of the ITGB3 gene among healthy individuals was found in 70%, among patients with diabetic foot – 60.3%. The T/C genotype of the studied polymorphism of the ITGB3 gene in the control group was recorded in 29%, in the group of patients in 37.7%. The C/C genotype was detected in 1% of healthy individuals and 2% of diabetic foot patients. The G/G genotype of the G1691A polymorphism of the FV gene was observed in 97% of healthy individuals and in 94% of patients among patients. The G/A genotype was detected in 3% in the control group and in 6% in the group of diabetic foot patients. The homozygous A/A genotype was not registered in the studied groups. The G/G genotype of the G20210A polymorphism of the FII gene was registered in 92% of healthy individuals and in 95.5% of patients with diabetic foot. The G/A genotype was detected in 8% of cases in the control group and 4.5% of cases among patients with diabetic foot. The homozygous A/A genotype was not detected in the examined groups. Conclusion: we did not find significant differences in the frequency of occurrence of genotype variants and alleles of the studied polymorphisms of the ITGB3, FV, FII genes among healthy individuals and patients with diabetic foot living in the Trans-Baikal Territory.