Analysis of the role of Aurora B on the chromosomal targeting of condensin I

Takemoto, Ai; Murayama, Akiko; Katano, Miyuki; Urano, Takeshi; Furukawa, Koichi; Yokoyama, Shigeyuki; Yanagisawa, Junn; Hanaoka, Fumio; Kimura, Kazuhiro

doi:10.1093/nar/gkm157

Cited by 58 publications

(52 citation statements)

References 43 publications

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“…However, the extent of the functional contribution of aurora B to chromosomal loading of condensins appears to vary among different species or cell types. For example, depletion of aurora B from Xenopus egg extracts barely or only subtly affects the level of condensin I loaded onto metaphase chromosomes and the resulting chromosome morphology (MacCallum et al 2002;Takemoto et al 2007). In HeLa cells, inhibition or depletion of aurora B reduces the amount of condensin I, but not of condensin II, loaded onto chromosomes in prometaphase (Lipp et al 2007).…”

Section: Aurora Bmentioning

confidence: 99%

Condensins: universal organizers of chromosomes with diverse functions

2012

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Condensins are multisubunit protein complexes that play a fundamental role in the structural and functional organization of chromosomes in the three domains of life. Most eukaryotic species have two different types of condensin complexes, known as condensins I and II, that fulfill nonoverlapping functions and are subjected to differential regulation during mitosis and meiosis. Recent studies revealed that the two complexes contribute to a wide variety of interphase chromosome functions, such as gene regulation, recombination, and repair. Also emerging are their cell type- and tissue-specific functions and relevance to human disease. Biochemical and structural analyses of eukaryotic and bacterial condensins steadily uncover the mechanisms of action of this class of highly sophisticated molecular machines. Future studies on condensins will not only enhance our understanding of chromosome architecture and dynamics, but also help address a previously underappreciated yet profound set of questions in chromosome biology.

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Section: Aurora Bmentioning

confidence: 99%

Condensins: universal organizers of chromosomes with diverse functions

2012

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“…The apparently stronger, albeit diffuse, INCENP signal on condensin I compared with condensin II KO metaphase chromosomes probably relates to the more diffuse nature of CAP-H OFF chromatin compared with CAP-D3 OFF chromatin. Studies of vertebrates in vivo using the aurora B inhibitor hesperadin and in vitro data depleting aurora B using a cell-free system both showed disruption of the chromosome passenger complex causing inhibition of loading of condensin I, but not condensin II, onto mitotic chromosomes I (Lipp et al, 2007;Takemoto et al, 2007). These studies and our data suggest that, although only condensin I is dependent on the chromosome passenger complex (CPC) for correct loading onto chromosomes, the CPC requires the action of both condensins for its localisation to the inner centromere of mitotic chromosomes.…”

Section: Cap-h Ko Cells Fail Cytokinesis Due To Persistence Of Anaphamentioning

confidence: 99%

Contrasting roles of condensin I and II in mitotic chromosome formation

Green

Kalitsis

Chang

et al. 2012

Journal of Cell Science

176

190

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SummaryIn vertebrates, two condensin complexes exist, condensin I and condensin II, which have differing but unresolved roles in organizing mitotic chromosomes. To dissect accurately the role of each complex in mitosis, we have made and studied the first vertebrate conditional knockouts of the genes encoding condensin I subunit CAP-H and condensin II subunit CAP-D3 in chicken DT40 cells. Live-cell imaging reveals highly distinct segregation defects. CAP-D3 (condensin II) knockout results in masses of chromatincontaining anaphase bridges. CAP-H (condensin I)-knockout anaphases have a more subtle defect, with chromatids showing fine chromatin fibres that are associated with failure of cytokinesis and cell death. Super-resolution microscopy reveals that condensin-Idepleted mitotic chromosomes are wider and shorter, with a diffuse chromosome scaffold, whereas condensin-II-depleted chromosomes retain a more defined scaffold, with chromosomes more stretched and seemingly lacking in axial rigidity. We conclude that condensin II is required primarily to provide rigidity by establishing an initial chromosome axis around which condensin I can arrange loops of chromatin.

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“…In some experimental systems, Aurora B depletion has no effect on the chromosomal targeting of condensin(s) MacCallum et al, 2002;Ono et al, 2004). However, in other studies, depletion of Aurora B leads to defects in loading of the single yeast condensin in fission yeast (Petersen and Hagan, 2003) and defects of condensin I loading in Drosophila (Giet and Glover, 2001), Xenopus (Takemoto et al, 2007) and HeLa cells (Lipp et al, 2007). Interestingly, condensin II in HeLa cells was unaffected (Lipp et al, 2007), indicating that Aurora B might preferentially affect condensin I targeting in mitosis.…”

Section: Introductionmentioning

confidence: 97%

Different roles for Aurora B in condensin targeting during mitosis and meiosis

Collette

Petty

Golenberg

et al. 2011

Journal of Cell Science

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SummaryCondensin complexes are essential for mitotic and meiotic chromosome segregation. Caenorhabditis elegans, like other metazoans, has two distinct mitotic and meiotic condensin complexes (I and II), which occupy distinct chromosomal domains and perform nonredundant functions. Despite the differences in mitotic and meiotic chromosome behavior, we uncovered several conserved aspects of condensin targeting during these processes. During both mitosis and meiosis, condensin II loads onto chromosomes in early prophase, and condensin I loads at entry into prometaphase. During both mitosis and meiosis, the localization of condensin I, but not condensin II, closely parallels the localization of the chromosomal passenger kinase Aurora B (AIR-2 in C. elegans). Interestingly, condensin I and AIR-2 also colocalize on the spindle midzone during anaphase of mitosis, and between separating chromosomes during anaphase of meiosis. Consistently, AIR-2 affects the targeting of condensin I but not condensin II. However, the role AIR-2 plays in condensin I targeting during these processes is different. In mitosis, AIR-2 activity is required for chromosomal association of condensin I. By contrast, during meiosis, AIR-2 is not required for condensin I chromosomal association, but it provides cues for correct spatial targeting of the complex.

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Analysis of the role of Aurora B on the chromosomal targeting of condensin I

Cited by 58 publications

References 43 publications

Condensins: universal organizers of chromosomes with diverse functions

Condensins: universal organizers of chromosomes with diverse functions

Contrasting roles of condensin I and II in mitotic chromosome formation

Different roles for Aurora B in condensin targeting during mitosis and meiosis

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