Analysis of the role of transcription factor VAD-5 in conidiation of Neurospora crassa

Sun, Xiepeng; Yu, Luning; Li, Nan; Wei, Shiping; Yu, Yufei; Zhang, Hanxing; Zhang, Xinyu; Li, Shaojie

doi:10.1016/j.fgb.2012.03.003

Cited by 30 publications

(26 citation statements)

References 34 publications

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“…One of these genes, bcltf2 , encodes a putative C2H2‐TF with described orthologues that are known to be involved in developmental processes. Mutants of N. crassa lacking the orthologue SAH‐1 ( short aerial hyphae‐1 ) produce shortened aerial hyphae and show an earlier and slightly increased conidial production (Colot et al ., ; Chen et al ., ; Sun et al ., ), whereas COS1 ( conidiophore stalk‐less 1 ) from M. oryzae is required for the formation of conidia (Zhou et al ., ). AZF1p ( asparagine‐rich zinc finger protein 1 ) from S. cerevisiae serves a dual function depending on the provided carbon source by regulating different sets of genes under fermentative and non‐fermentative growth (Slattery et al ., ).…”

Section: Discussionmentioning

confidence: 99%

Light governs asexual differentiation in the grey mould fungus Botrytis cinerea via the putative transcription factor BcLTF2

Cohrs

Simon

Viaud

et al. 2016

Environmental Microbiology

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Botrytis cinerea is a plant pathogenic fungus known for its utilization of light as environmental cue to regulate asexual differentiation: conidia are formed in the light, while sclerotia are formed in the dark. As no orthologues of known regulators of conidiation (e.g., Aspergillus nidulans BrlA, Neurospora crassa FL) exist in the Leotiomycetes, we initiated a de novo approach to identify the functional counterpart in B. cinerea. The search revealed the light-responsive C2H2 transcription factor BcLTF2 whose expression - usually restricted to light conditions - is necessary and sufficient to induce conidiation and simultaneously to suppress sclerotial development. Light-induced expression of bcltf2 is mediated via a so far unknown pathway, and is attenuated in a (blue) light-dependent fashion by the White Collar complex, BcLTF1 and the VELVET complex. Mutation of either component leads to increased bcltf2 expression and causes light-independent conidiation (always conidia phenotype). Hence, the tight regulation of bcltf2 governs the balance between vegetative growth that allows for the colonization of the substrate and subsequent reproduction via conidia in the light. The orthologue ssltf2 in the closely related species Sclerotinia sclerotiorum is not significantly expressed suggesting that its deregulation may cause the lack of the conidiation program in this fungus.

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Section: Discussionmentioning

confidence: 99%

Light governs asexual differentiation in the grey mould fungus Botrytis cinerea via the putative transcription factor BcLTF2

Cohrs

Simon

Viaud

et al. 2016

Environmental Microbiology

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“…Detailed analysis of available microarray data related to the antioxidant and redox systems of Neurospora is beyond the scope of this review. However, for example, the transcription factors VAD-5, CRE-1, and CPC-1 may be regulators of the ROS homeostasis (153,154,160). In addition, mutations in several serine-threonine protein kinases result in enhanced sensitivity to oxidative stress, suggesting the involvement of these kinases in the control of the antioxidant and redox system of Neurospora (123).…”

Section: Regulation Of the Antioxidant System In Neurospora Crassamentioning

confidence: 99%

Interconnections of Reactive Oxygen Species Homeostasis and Circadian Rhythm inNeurospora crassa

Gyöngyösi

Káldi

2014

Antioxidants & Redox Signaling

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Significance: Both circadian rhythm and the production of reactive oxygen species (ROS) are fundamental features of aerobic eukaryotic cells. The circadian clock enhances the fitness of organisms by enabling them to anticipate cycling changes in the surroundings. ROS generation in the cell is often altered in response to environmental changes, but oscillations in ROS levels may also reflect endogenous metabolic fluctuations governed by the circadian clock. On the other hand, an effective regulation and timing of antioxidant mechanisms may be crucial in the defense of cellular integrity. Thus, an interaction between the circadian timekeeping machinery and ROS homeostasis or signaling in both directions may be of advantage at all phylogenetic levels. Recent Advances: The Frequency-White Collar-1 and White Collar-2 oscillator (FWO) of the filamentous fungus Neurospora crassa is well characterized at the molecular level. Several members of the ROS homeostasis were found to be controlled by the circadian clock, and ROS levels display circadian rhythm in Neurospora. On the other hand, multiple data indicate that ROS affect the molecular oscillator. Critical Issues: Increasing evidence suggests the interplay between ROS homeostasis and oscillators that may be partially or fully independent of the FWO. In addition, ROS may be part of a complex cellular network synchronizing non-transcriptional oscillators with timekeeping machineries based on the classical transcription-translation feedback mechanism. Future Directions: Further investigations are needed to clarify how the different layers of the bidirectional interactions between ROS homeostasis and circadian regulation are interconnected. Antioxid. Redox Signal. 20,[3007][3008][3009][3010][3011][3012][3013][3014][3015][3016][3017][3018][3019][3020][3021][3022][3023]

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“…For example, mutants lacking the transcription factor fluffy [ fl / NCU08726 ; ( Bailey and Ebbole 1998 )] and the transcriptional adapter rco-1 / NCU06205 ( Yamashiro et al 1996 ) are blocked at distinct points in the process. Other work has demonstrated transcription factors that influence conidiation under different environmental conditions or the extent of conidiation, including vad-5 / NCU06799 ( Sun et al 2012 ), chc-1 / NCU00749 ( Sun et al 2011 ) and hsf-2 / NCU08480 ( Thompson et al 2008 ). The white collar complex (WCC) containing the transcription factors wc-1 / NCU02356 and wc-2/ NCU00902 has been demonstrated to directly control expression of 24 transcription factor genes under blue light regulation ( Smith et al 2010 ; Chen et al 2009 ).…”

Section: Resultsmentioning

confidence: 99%

Functional Profiling of Transcription Factor Genes inNeurospora crassa

Carrillo¹,

Schacht²,

Cabrera³

et al. 2017

G3 Genes|Genomes|Genetics

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Regulation of gene expression by DNA-binding transcription factors is essential for proper control of growth and development in all organisms. In this study, we annotate and characterize growth and developmental phenotypes for transcription factor genes in the model filamentous fungus Neurospora crassa. We identified 312 transcription factor genes, corresponding to 3.2% of the protein coding genes in the genome. The largest class was the fungal-specific Zn2Cys6 (C6) binuclear cluster, with 135 members, followed by the highly conserved C2H2 zinc finger group, with 61 genes. Viable knockout mutants were produced for 273 genes, and complete growth and developmental phenotypic data are available for 242 strains, with 64% possessing at least one defect. The most prominent defect observed was in growth of basal hyphae (43% of mutants analyzed), followed by asexual sporulation (38%), and the various stages of sexual development (19%). Two growth or developmental defects were observed for 21% of the mutants, while 8% were defective in all three major phenotypes tested. Analysis of available mRNA expression data for a time course of sexual development revealed mutants with sexual phenotypes that correlate with transcription factor transcript abundance in wild type. Inspection of this data also implicated cryptic roles in sexual development for several cotranscribed transcription factor genes that do not produce a phenotype when mutated.

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Analysis of the role of transcription factor VAD-5 in conidiation of Neurospora crassa

Cited by 30 publications

References 34 publications

Light governs asexual differentiation in the grey mould fungus Botrytis cinerea via the putative transcription factor BcLTF2

Light governs asexual differentiation in the grey mould fungus Botrytis cinerea via the putative transcription factor BcLTF2

Interconnections of Reactive Oxygen Species Homeostasis and Circadian Rhythm inNeurospora crassa

Functional Profiling of Transcription Factor Genes inNeurospora crassa

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