Analysis of the spontaneous<i>in vitro</i>anti-HIV-1 antibody secretion by peripheral blood mononuclear cells in HIV-1 infection

Vendrell, Jean-Pierre; Segondy, Michel; Ducos, J; Reynes, Jacques; Huguet, Marie-France; Nicolas, Jean‐Claude; Serre, A

doi:10.1111/j.1365-2249.1991.tb05614.x

Cited by 29 publications

(3 citation statements)

References 24 publications

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“…In individuals who progress toward AIDS the level of anti-core antibodies progressively falls [32,33]. In addition, longitudinal studies of the immune response in HIV-1-infected patients have demonstrated that the reduction in antibody reactivity to HIV-1 p24 and pl7 is an early predictor of disease progression [34][35][36]. The disappearance of anti-core antibody producing cells from peripheral blood during the latest phases of the disease could be an expression of the general deregulation of the immune system, with a decrease of T helper lymphocytes function, but also to a further sequestration of secreting cells at the sites of active viral replication.…”

Section: Discussionmentioning

confidence: 99%

In vitro anti-HIV-1 antibody production in subjects in different stages of HIV-1 infection

Rusconi

Riva

Meroni

et al. 1995

Clinical and Experimental Immunology

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SUMMARY We evaluated the in vitro antibody production from peripheral blood mononuclear cells (PBMC) against HIV‐1 proteins in infected adults. Fifty‐four HIV‐1 infected patients (four recent seroconverters, 15 asymptomatics with a CD4 count higher than 500/μl, 27 asymptomatics with a CD4 count between 200 and 500/μl and eight symptomatic patients) were tested. PBMC were incubated in the presence or absence of 1% pokeweed mitogen (PWM) at 37°C for 8 days. Western blot assay, p24 antigen ELISA and anti‐p24 antibody ELISA were performed on serum and culture supernatants. Spontaneous production of anti‐env antibody in culture supernatants was evidenced in all subjects. All the positive supernatants for anti‐core antibodies (18/54) were derived from asymptomatic patients. PBMC from recent seroconverters and from symptomatic patients did not produce any anti‐core antibody. Antibody production decreased after stimulation with PWM. The concentration of p24 antigen did not significantly increase in p24 positive supernatants following acidification (P= 0·1), suggesting that the inability to detect p24 antibody was not due to the anti‐p24 antibody complexed to p24 antigen in culture supernatants. In vitro production of anti‐p24 antibodies was significantly more frequent in asymptomatic subjects with high CD4+ cell counts (P= 002) and was absent in recent seroconverters. This last finding suggests that during the initial phases of the infection, anti‐p24 antibody production may be restricted to cells residing in lymphoid organs. In addition, the lower percentage of anti‐core antibody in people with low CD4+ cell counts is not merely a consequence of the binding of the antibody to an increased amount of antigen, but probably reflects an impaired production or a sequestration of producing cells in lymphoid tissue during the late stages of the infection.

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Section: Discussionmentioning

confidence: 99%

In vitro anti-HIV-1 antibody production in subjects in different stages of HIV-1 infection

Rusconi

Riva

Meroni

et al. 1995

Clinical and Experimental Immunology

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“…detection of HIV-spccifk antibody immunocompromised patients such as children infeeted in utero ^^^^^f«" ^y PBMC has been put forward as a valuable tool for [I] and organ-transplant recipients [2.3] or patients with AIDS '^«"fi'-'^^"on of early HIV infection in subjects at risk [18][19][20]. MJ We therefore wondered whether T. gondii infection would Diagnosis of the acute phase of 7. gondii infection is induce a specific antibody secretion by PBMC and whether the generally based on the detection of T. gondii-spcdnc IgM and detection of this produetion might help to d.stmguish unambiIgG antibodies.…”

Section: Introduction Blood Mononuclear Cells (Pbmc) Has Been Well Domentioning

confidence: 99%

“…Several investigators consider the detection of T. gondH-spcdfic IgA [6][7][8] and more recently IgE [9] antibodies in serum as a SUBJECTS AND METHODS useful additional criterion to date the parasite contamination. Detection of antigen-specific antibody secreting peripheral Subjects Fifty-six patients were divided into three groups on the basis of Correspondence: Dr J. P. Vendrell Cell preparatitin and culture conditions PBMC culiure supernatants were prepared as previously described [20]. PBMC (5x 10*/ml) from three randomly selected women of group A were eultured for 6 days with 50 ^g/ml of cyctoheximide (Boerhinger Mannheitn, Marburg, Germany).…”

Section: Introduction Blood Mononuclear Cells (Pbmc) Has Been Well Domentioning

confidence: 99%

Secretion of Toxoplasma gondii-specfic antibody in vitro by peripheral blood mononuclear cells as a new marker of acute toxoplasmosis

Vendrell

Pratlong

Decoster

et al. 1992

Clinical and Experimental Immunology

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SUMMARYAntigen-specific antibody secretion in vitro by peripheral blood mononuclear cells (PBMC) reflects an in vivo stimulation of the immune system by the antigen. Primary infection of immunoeompetent patients with 7. g^ow/n causes an acute infection followed by chronic toxoplasmosis. We examined in vitro anti-Toxopiasma antibody production by PBMC during the acute and ehronic phases of toxoplasmosis. PBMC from patients with acute or chronic toxoplasmosis and scronegative subjects were cultured for up to 6 days. Anti-Toxoplastna antibodies were assayed in supernatants by ELISA and immunoblotting. Anti-Toxoplasma antibodies were detected in supernatants of PBMC from 29 pregnant women who seroeonverted during gestation. PBMC from 17 patients who had chronic toxoplasmosis and PBMC from 10 seronegative healthy controls did not secrete Toxoplasma-specific antibodies. This in vitro antibody secretion was spontaneous, active and transient since it disappeared between 11 and 24 weeks after seroeonversion. Anti-Toxoplasma antibody secretion by PBMC from patients with acute toxoplasmosis is consistent with an in vii'o stimulation of the immune system by T. gondii antigens. Our results represent a new approach for studying the immunological response during T. gondii infection and could have important implications for the diagnosis of acute and re-activated toxoplasmosis.

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Cytomegalovirus‐specific in vitro antibody production by peripheral blood lymphocytes from renal transplant recipients with CMV infection

Segondy

Barat

Mourad

et al. 1993

Journal of Medical Virology

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Cytomegalovirus-specific in vitro antibody production (CMV-IVAP) by peripheral blood lymphocytes (PBL) was investigated in 12 renal transplant recipients. CMV-IVAP, CMV-serology, and viral cultures were carried out weekly during at least 8 weeks after transplantation. Nine episodes of CMV infection occurred in eight patients. CMV-IVAP was positive in eight episodes; IgG and/or IgA and/or IgM antibodies reacting with several CMV polypeptides were secreted by PBL. In two patients with primary CMV infection, only IgA antibodies were detected. Cytomegalovirus cultures were positive in eight episodes and serological evidence of CMV infection was obtained in five episodes. In one case, CMV-IVAP was observed before CMV isolation and in another case, before serological evidence of CMV infection. Our study indicates that CMV-IVAP could represent an additional tool for the diagnosis of CMV infection. Our study indicates that CMV-IVAP could represent an additional tool for the diagnosis of CMV infection in renal transplant recipients.

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Analysis of the spontaneousin vitroanti-HIV-1 antibody secretion by peripheral blood mononuclear cells in HIV-1 infection

Cited by 29 publications

References 24 publications

In vitro anti-HIV-1 antibody production in subjects in different stages of HIV-1 infection

In vitro anti-HIV-1 antibody production in subjects in different stages of HIV-1 infection

Secretion of Toxoplasma gondii-specfic antibody in vitro by peripheral blood mononuclear cells as a new marker of acute toxoplasmosis

Cytomegalovirus‐specific in vitro antibody production by peripheral blood lymphocytes from renal transplant recipients with CMV infection

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