Analysis of the structure and expression pattern of MRP7 (ABCC10), a new member of the MRP subfamily

Hopper, Elizabeth; Belinsky, Martin G.; Zeng, Hao; Tosolini, Alessandra; Testa, Joseph R.; Kruh, Gary D.

doi:10.1016/s0304-3835(00)00646-7

Cited by 164 publications

(162 citation statements)

References 42 publications

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“…Internet communication, http://nutrigene.4t.com/humanabc.htm). We identified seven known transporter genes: ABCB6 (ABCB6) (Allikmets et al, 1996), ABCB2 (TAP1), ABCB3 (TAP2) (Bahram et al, 1991), ABCC10 (MRP7) (Hopper et al, 2001), ABCF1 (ABC50) (Richard et al, 1998), ABCA2 (ABC2) (Vulevic et al, 2001) and ABCC4 (MRP4) (Kool et al, 1997) (see Table 3). …”

Section: Resultsmentioning

confidence: 99%

“…Internet communication, http://nutrigene.4t.com/ humanabc.htm). Reverse transcriptase -polymerase chain reaction for four transporter genes, ABCB6 (Allikmets et al, 1996), ABCF1 (Richard et al, 1998), ABCC4 (Kool et al, 1997) and ABCC10 (Hopper et al, 2001), and immunohistochemistry of two genes, ABCB2 and ABCB3 (Bahram et al, 1991), showed similar levels of mRNA and protein, respectively, in GLC4-MITO and GLC4.…”

Section: Discussionmentioning

confidence: 99%

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Mitoxantrone resistance in a small cell lung cancer cell line is associated with ABCA2 upregulation

Boonstra¹,

Timmer‐Bosscha

Echten-Arends

et al. 2004

Br J Cancer

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The aim of this study was to find factors that could explain the accumulation difference of mitoxantrone in the BCRP1-negative GLC4-MITO cell line compared to GLC4. Comparative genomic hybridisation (CGH) was applied to determine chromosomal differences between GLC4 and GLC4-MITO. Comparative genomic hybridisation analysis revealed gain of 2q, 6p, 9q, 13q, 14q, 15q, 19q and Xp and loss of 1p, 2q, 3p, 3q, 4q, 6q, 8q, 11p, 16p, 17q, 18p, 20p and Xq. In the over-represented chromosomal areas, seven transporter genes were identified: ABCB6, ABCB2 (TAP1), ABCB3 (TAP2), ABCF1 (ABC50), ABCC10 (MRP7), ABCA2 (ABC2) and ABCC4 (MRP4). No RNA or protein upregulation was observed for ABCB6, ABCF1, ABCC10, ABCC4, ABCB2 and ABCB3, but an increased expression was detected for ABCA2 mRNA in GLC4-MITO. ABCA2 is known to be involved in resistance to estramustine. In the MTT assay, GLC4-MITO was two-fold resistant to estramustine compared to GLC4. Coincubation with estramustine and mitoxantrone increased mitoxantrone accumulation in GLC4-MITO, while this was not affected in GLC4. This suggests that estramustine is able to block mitoxantrone efflux in GLC4-MITO cells. These data reveal that cellular reduction of mitoxantrone in a mitoxantrone-resistant cell line is associated with overexpression of ABCA2.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Mitoxantrone resistance in a small cell lung cancer cell line is associated with ABCA2 upregulation

Boonstra¹,

Timmer‐Bosscha

Echten-Arends

et al. 2004

Br J Cancer

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“…Analyses of the predicted structures of subsequently identified MRPs indicated that not all of them possess MSD0 domains . MRP4, MRP5 and the recently identified MRP8 and MRP9 lack the third membrane spanning domain (MSD0), but possess L0 domains, whereas MRP2, MRP3, MRP6 and MRP7 resemble MRP1 (Bera et al, 2001;Hopper et al, 2001;Tammur et al, 2001;Yabuuchi et al, 2001) (Figure 1). Remarkably, all of the characterized members of this family are lipophilic anion pumps that have the facility for conferring resistance to anticancer agents.…”

Section: The Mrp Familymentioning

confidence: 99%

The MRP family of drug efflux pumps

2003

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The MRP family is comprised of nine related ABC transporters that are able to transport structurally diverse lipophilic anions and function as drug efflux pumps. Investigations of this family have provided insights not only into cellular resistance mechanisms associated with natural product chemotherapeutic agents, antifolates and nucleotide analogs, but also into factors that influence drug distribution in the body, membrane systems that are involved in the extrusion of reduced folates, cysteinyl leukotrienes and bile acids, and the molecular basis of two hereditary conditions in humans. The review will describe the biochemical properties, drug resistance activities and potential in vivo functions of these unusual pumps.

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“…A t least seven members of the multidrug resistance protein (MRP) subfamily of ATP binding cassette (ABC) transporters, called MRP1-7, have been identified in humans Hopper et al, 2001;Kool et al, 1997). Until recently, only MRP1 and MRP2 were shown to confer resistance to a wide variety of chemotherapeutic agents in human cancer cells (Cole and Deeley, 1998;Cole et al, 1992;Cui et al, 1999;Evers et al, 2000).…”

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confidence: 99%

Tissue Distribution and Induction of Human Multidrug Resistant Protein 3

Scheffer

Kool

Haas

et al. 2002

Laboratory Investigation

244

156

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SUMMARY:The multidrug resistance protein (MRP) family consists of several members and, for some of these transporter proteins, distinct roles in multidrug resistance and normal tissue functions have been well established (MRP1 and MRP2) or are still under investigation (MRP3). MRP3 expression studies in human tissues have been largely restricted to the mRNA level. In this report we extended these studies and further explored MRP3 expression at the protein level. Western blot and immunohistochemistry with two MRP3-specific monoclonal antibodies, M 3 II-9 and M 3 II-21, showed MRP3 protein to be present in adrenal gland, and kidney and in tissues of the intestinal tract: colon, pancreas, gallbladder, and liver. In epithelia, MRP3 was found to be located at the basolateral sides of cell membranes. In normal liver, MRP3 was detected at lower levels than anticipated from the mRNA data and was found present mainly in the bile ducts. In livers from patients with various forms of cholestasis, MRP3 levels were frequently increased in the proliferative cholangiocytes, with sometimes additional staining of the basolateral membranes of the hepatocytes. This was especially evident in patients with type 3 progressive familial intrahepatic cholestasis. The present results support the view that MRP3 plays a role in the cholehepatic and enterohepatic circulation of bile and in protection within the biliary tree and tissues along the bile circulation route against toxic bile constituents. The possible functional roles for MRP3 in the adrenal gland and in the kidney remain as yet unknown. In a panel of 34 tumor samples of various histogenetic origins, distinct amounts of MRP3 were detected in a limited number of cases, including lung, ovarian, and pancreatic cancers. These findings may be of potential clinical relevance when considering the drug treatment regimens for these tumor types. (Lab Invest 2002, 82:193-201).

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Analysis of the structure and expression pattern of MRP7 (ABCC10), a new member of the MRP subfamily

Cited by 164 publications

References 42 publications

Mitoxantrone resistance in a small cell lung cancer cell line is associated with ABCA2 upregulation

Mitoxantrone resistance in a small cell lung cancer cell line is associated with ABCA2 upregulation

The MRP family of drug efflux pumps

Tissue Distribution and Induction of Human Multidrug Resistant Protein 3

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