Analysis of the structure of microbial community in soils with different degrees of salinization using T-RFLP and real-time PCR techniques

Andronov, E. E.; Петрова, С.Н.; Пинаев, А. Г.; Pershina, E. V.; Rakhimgalieva, S. Zh.; Ахмеденов, К. М.; Gorobets, A.; Sergaliev, N. Kh.

doi:10.1134/s1064229312020044

Cited by 72 publications

(22 citation statements)

References 26 publications

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“…This was an indication of increased osmotic pressure (salt stress), that would possibly make the substrate draw water out of the cells of microorganism, hence death in the media or reduced chance of survival. This observation is in agreement with Andronov et al (2012), that salt stress can reduce microbial activities, biomass and community structures in soil or substrate as in this case. Results are also in agreement with the observation of Yan et al (2015) on the reduction of soil microbes with the influence of soluble salts in the substrate.…”

Section: Discussionsupporting

confidence: 93%

Suitability of biosolids from university sewage ponds as a substrate for crop production

Otieno¹,

Nyalala²,

Wolukau³

2019

Afr. J. Agric. Res.

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Currently, sewage waste management is a serious environmental problem and one of the major growing concerns for urban areas all over the world. Utilization of biosolids (BS) for crop production may be a sustainable waste management strategy. The present study evaluated the physico-chemical and biological characteristics of biosolids from sewage ponds at Egerton University, Kenya. This was to determine its suitability for crop production. Biosolids were evaluated separately then as mixture with forest soil at rates of 0, 10, 20, 30, 40, 50 and 60% and compared with tea compost (TC) and coco peat (CP) in a completely randomized design experiment with four replications. Data collected included: macro-elements, micro-elements, heavy metals, pH, electrical conductivity (EC), bulk density (BD), water holding capacity and biological properties. Results showed that total organic carbon (0.03%), total organic nitrogen (2.0%) and Molybdenum (22 mg kg-1), in biosolids were significantly (p < 0.05) higher compared with forest soil, but not significantly different from tea compost. For heavy metals, Hg (0.33 mg kg-1), As (5.9 mg kg-1), Cr (31.1mg kg-1), Cd (0.38 mg kg-1), Ni (16.3 mg kg-1) and Zn (127 mg kg-1) were significantly (p < 0.05) higher in biosolids but within the allowable limits according to Environmental Protection Agency (EPA) standards. bulk density (1.2 to 1.5) g cm-3 and pH (5.4 to 5.8) units, but high organic matter (195 to 230) g kg-1, water holding capacity (35 to 42 %) and EC (2.6 to 5.4) µSm-1). For microbial load, total viable count (TVC) and colony forming units (CFU) registered 5 ×10-7 and 6.5×10-7 respectively. However, Escherichia coli, Salmonella sp. and Staphylococcus sp. were not detectable in the fully composted biosolids. Similar trend of these results were subsequently observed in the substrates formed in the mixture of biosolids and forest soil and this provide insight on the potential of biosolids as substrate for crop production and a reliable alternative to soil alone.

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Section: Discussionsupporting

confidence: 93%

Suitability of biosolids from university sewage ponds as a substrate for crop production

Otieno¹,

Nyalala²,

Wolukau³

2019

Afr. J. Agric. Res.

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“…It is agreed in the literature that the driving factors for the evolution of a microbial community structure largely depend on the environmental gradient to which the communities are subjected or imposed under experimentally 62 . Organic C 63 , N availability 64 , pH 65 , salinity 66 67 , moisture 68 and many other abiotic factors influence the microbial community structure in soil. Despite this, the edaphic gradients were not found to equally impact the community structure in the tailings of this study, with soluble N and water content being the best controllers.…”

Section: Discussionmentioning

confidence: 99%

From lithotroph- to organotroph-dominant: directional shift of microbial community in sulphidic tailings during phytostabilization

Bond

Nostrand

et al. 2015

Sci Rep

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Engineering microbial diversity to enhance soil functions may improve the success of direct revegetation in sulphidic mine tailings. Therefore, it is essential to explore how remediation and initial plant establishment can alter microbial communities, and, which edaphic factors control these changes under field conditions. A long-term revegetation trial was established at a Pb-Zn-Cu tailings impoundment in northwest Queensland. The control and amended and/or revegetated treatments were sampled from the 3-year-old trial. In total, 24 samples were examined using pyrosequencing of 16S rRNA genes and various chemical properties. The results showed that the microbial diversity was positively controlled by soil soluble Si and negatively controlled by soluble S, total Fe and total As, implying that pyrite weathering posed a substantial stress on microbial development in the tailings. All treatments were dominated by typical extremophiles and lithotrophs, typically Truepera, Thiobacillus, Rubrobacter; significant increases in microbial diversity, biomass and frequency of organotrophic genera (typically Nocardioides and Altererythrobacter) were detected in the revegetated and amended treatment. We concluded that appropriate phytostabilization options have the potential to drive the microbial diversity and community structure in the tailings toward those of natural soils, however, inherent environmental stressors may limit such changes.

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“…Previous studies ( 2 , 3 , 7 , 17 , 22 ) demonstrated that high salinity reduces microbial activity and changes the microbial community structure. Kartal et al ( 9 ) reported two possible outcomes of the adaptation of a biomass to salinity: (i) the acclimation of the existing population or (ii) a population shift.…”

mentioning

confidence: 93%

Effects of Salts on the Activity and Growth of “<i>Candidatus</i> Scalindua sp.”, a Marine Anammox Bacterium

et al. 2018

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Four salts, SEALIFE (a synthetic sea salt), NaCl, Na2SO4, and NaCl+KCl, were applied to monitor the effects of salinity on “Candidatus Scalindua sp.”, a marine anaerobic ammonium oxidation (anammox) bacterium. The highest ammonium consumption of 10 μmol mg protein−1 d−1 was observed at 88 mmol L−1 of Na in the presence of NaCl. The highest inorganic carbon uptake of 0.6 μmol mg protein−1 d−1 was observed at 117 mmol L−1 of Na and at 16 mmol L−1 of K in the presence of NaCl+KCl. Thus, Na and K are both important for maintaining a high growth rate of “Candidatus Scalindua sp.”

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Analysis of the structure of microbial community in soils with different degrees of salinization using T-RFLP and real-time PCR techniques

Cited by 72 publications

References 26 publications

Suitability of biosolids from university sewage ponds as a substrate for crop production

Suitability of biosolids from university sewage ponds as a substrate for crop production

From lithotroph- to organotroph-dominant: directional shift of microbial community in sulphidic tailings during phytostabilization

Effects of Salts on the Activity and Growth of “<i>Candidatus</i> Scalindua sp.”, a Marine Anammox Bacterium

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