Analysis of the transcriptome and metabolome of pancreatic spheroids derived from human induced pluripotent stem cells and matured in an organ-on-a-chip

Abstract

Functional differentiation of pancreatic like tissue from human induced pluripotent stem cells is one of the emerging strategies to achieve in vitro pancreas model. Here, we propose a protocol to...

Generation of β-like cell subtypes from differentiated human induced pluripotent stem cells in 3D spheroids

“…In our previous work, we have proposed a protocol to generate C-peptide and insulin secreting β-like cells in spheroids derived from hiPSCs. 16,17 We noticed that our spheroids contained also cells positive to glucagon. A bulk transcriptomic investigation revealed that the spheroids co-expressed mRNA related to several hormones’ activities (such as IAPP, INS, INHBB, GCG, 17 and SST 16 ) when compared to the control of undifferentiated cells.…”

“…16,17 We noticed that our spheroids contained also cells positive to glucagon. A bulk transcriptomic investigation revealed that the spheroids co-expressed mRNA related to several hormones’ activities (such as IAPP, INS, INHBB, GCG, 17 and SST 16 ) when compared to the control of undifferentiated cells. Those results illustrated a potential cell heterogeneity.…”

“…Those results illustrated a potential cell heterogeneity. The cells also over expressed several genes of the lipids' metabolism (LDLR, SCD, HMGCR, HMGCS1) and various specific transcription factors (HIC2, WRNIP1, SOX8, FOX genes 17 ). In order to clarify the composition of our pancreatic model, we proposed in this work an additional investigation consisting of single cell sequencing analysis to extract the b-cell subpopulations.…”