Analysis of the Transcriptome of Blowfly Chrysomya megacephala (Fabricius) Larvae in Responses to Different Edible Oils

Zhang, Min; Yu, Hao; Yang, Yanyan; Chen, Song; Hu, Xinjun; Zhang, Guren

doi:10.1371/journal.pone.0063168

Cited by 16 publications

(10 citation statements)

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“…Developmental transcriptomes were established of eggs, mix-aged larvae, and mix-aged adults, providing a relatively comprehensive gene pool of C. megacephala. The number of clean reads in larva transcriptome was 34347518, which was similar to that of a reported larval transcriptome of C. megacephala [ 31 ]. And the number of clean reads from egg and adult transcriptome were 34,716,158, and 35,560,603, respectively.…”

Section: Discussionsupporting

confidence: 77%

“…Molecular progress of this species will provide important inroads to the discovery of novel target sites for population control, understanding of the immune response in this necrophagous fly. Transcriptome information and the differentially expressed genes related to lipometabolism in response to different kinds of oils were reported in larvae [ 23 ]. Despite efforts by developmental biologists, there is little molecular data regarding eggs and adults.…”

Section: Introductionmentioning

confidence: 99%

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The developmental transcriptome of the synanthropic fly Chrysomya megacephala and insights into olfactory proteins

et al. 2015

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BackgroundChrysomya megacephala (Fabricius) is a prevalent and synanthropic blowfly which has two sides, for being a pathogenic vector, an efficient pollinator, a promising resource of proteins, lipids, chitosan, biofuel et al., and an important forensic indicator. Moreover olfactory proteins are crucial component to function in related processes. However, the genomic platform of C. megacephala remains relatively unavailable. Developmental transcriptomes of eggs, larvae from 1st instar to before pupa stage and adults from emergence to egg laying period were built by RNA-sequencing to establish sequence background of C. megacephala with special lights on olfactory proteins.ResultsClean reads in eggs, larvae and adults were annotated into 59486 transcripts. Transcripts were assembled into 22286, 17180, 18934 and 35900 unigenes in eggs, larvae, adults and the combined datasets, respectively. Unigenes were annotated using Nr (NCBI non-redundant protein sequences), Nt (NCBI non-redundant nucleotide sequences), GO (Gene Ontology), PFAM (Protein family), KOG/COG (Clusters of Orthologous Groups of proteins), Swiss-Prot (A manually annotated and reviewed protein sequence database), and KO (KEGG Orthology). Totally 12196 unigenes were annotated into 51 sub-categories belonging to three main GO categories; 8462 unigenes were classified functionally into 26 categories to KOG classifications; 5160 unigenes were functionally classified into 5 KEGG categories. Moreover, according to RSEM, the number of differentially expressed genes between larvae and eggs, adults and eggs, adults and larvae, and the common differentially expressed genes were 2637, 1804, 2628 and 258, respectively. Among them, 17 odorant-binding proteins (OBPs), 7 chemosensory proteins (CSPs) and 8 ionotropic receptors (IRs) were differently expressed in adults and larvae. Ten were confirmed as significant differentially expressed genes. Furthermore, OBP Cmeg32081-c4 was highly expressed in the female head and Cmeg33593_c0 were up-regulated with the increase of larval age.ConclusionsA comprehensive sequence resource with desirable quality was built by comparative transcriptome of eggs, larvae and adults, enriching the genomic platform of C. megacephala. The identified differentially expressed genes would facilitate the understanding of metamorphosis, development and the fitness to environmental change of C. megacephala. OBP Cmeg32081-c4 and Cmeg33593_c0 might play a crucial role in the interactions between olfactory system and biological processes.Electronic supplementary materialThe online version of this article (doi:10.1186/s12864-014-1200-y) contains supplementary material, which is available to authorized users.

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Section: Discussionsupporting

confidence: 77%

Section: Introductionmentioning

confidence: 99%

The developmental transcriptome of the synanthropic fly Chrysomya megacephala and insights into olfactory proteins

et al. 2015

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“…Classification of predicted functions of the male and female C. stygia transcripts via GO assignment produced similar results as those obtained for other invertebrates [ 50 , 52 , 62 , 86 , 87 ]. The number of individual candidate transcripts identified for many of the olfactory gene families is also comparable to those of other Dipteran, Coleopteran, and Lepidopteran species for which the antennal transcriptome has been examined [ 50 , 51 , 86 , 88 - 91 ].…”

Section: Discussionsupporting

confidence: 73%

Chemosensory genes identified in the antennal transcriptome of the blowfly Calliphora stygia

et al. 2015

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BackgroundBlowflies have relevance in areas of forensic science, agriculture, and medicine, primarily due to the ability of their larvae to develop on flesh. While it is widely accepted that blowflies rely heavily on olfaction for identifying and locating hosts, there is limited research regarding the underlying molecular mechanisms. Using next generation sequencing (Illumina), this research examined the antennal transcriptome of Calliphora stygia (Fabricius) (Diptera: Calliphoridae) to identify members of the major chemosensory gene families necessary for olfaction.ResultsRepresentative proteins from all chemosensory gene families essential in insect olfaction were identified in the antennae of the blowfly C. stygia, including 50 odorant receptors, 22 ionotropic receptors, 21 gustatory receptors, 28 odorant binding proteins, 4 chemosensory proteins, and 3 sensory neuron membrane proteins. A total of 97 candidate cytochrome P450s and 39 esterases, some of which may act as odorant degrading enzymes, were also identified. Importantly, co-receptors necessary for the proper function of ligand-binding receptors were identified. Putative orthologues for the conserved antennal ionotropic receptors and candidate gustatory receptors for carbon dioxide detection were also amongst the identified proteins.ConclusionsThis research provides a comprehensive novel resource that will be fundamental for future studies regarding blowfly olfaction. Such information presents potential benefits to the forensic, pest control, and medical areas, and could assist in the understanding of insecticide resistance and targeted control through cross-species comparisons.Electronic supplementary materialThe online version of this article (doi:10.1186/s12864-015-1466-8) contains supplementary material, which is available to authorized users.

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“…Publication Ch. megacephala SRR620248 and SRR1663113 49,50 Co. hominivorax SRP044071 51 Figure 1. Assembly completeness.…”

Section: Species Ncbi Accessionmentioning

confidence: 99%

Functional genomics of the parasitic habits of blowflies and botflies

Cardoso

Deszo

Torres

2019

Preprint

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Infestation by dipterous larvaemyiasis -is a major problem for livestock industries worldwide and can cause severe economic losses. The Oestroidea superfamily is an interesting model to study the evolution of myiasis-causing flies because of the diversity of parasitism strategies among closely-related species. These flies are saprophagous, obligate parasites, or facultative parasites and can be subdivided into cutaneous, nasopharyngeal, traumatic, and furuncular. We expect that closely-related species have genetic differences that lead to the diverse parasitic strategies. To identify genes with such differences, we used gene expression and coding sequence data from five species (Cochliomyia hominivorax, Chrysomya megacephala, Lucilia cuprina, Dermatobia hominis, and Oestrus ovis). We tested whether 1,287 orthologs have different expression and evolutionary constraints under different scenarios. We found two up-regulated genes; one in species causing cutaneous myiasis that is involved in iron transportation/metabolization (ferritin), and another in species causing traumatic myiasis that responds to reduced oxygen levels (anoxia up-regulated-like). Our evolutionary analysis showed a similar result. In the Ch. hominivorax branch, we found one gene with the same function as ferritin that may be evolving under positive selection, spook. This is the first step towards understanding origins and evolution of parasitic strategy diversity in Oestroidea.

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Analysis of the Transcriptome of Blowfly Chrysomya megacephala (Fabricius) Larvae in Responses to Different Edible Oils

Cited by 16 publications

References 40 publications

The developmental transcriptome of the synanthropic fly Chrysomya megacephala and insights into olfactory proteins

The developmental transcriptome of the synanthropic fly Chrysomya megacephala and insights into olfactory proteins

Chemosensory genes identified in the antennal transcriptome of the blowfly Calliphora stygia

Functional genomics of the parasitic habits of blowflies and botflies

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