Analysis of the upstream genetic structures of the ISEcp1‐bla_CTX‐M transposition units in Escherichia coli isolates carrying bla_CTX‐M obtained from the Indonesian and Vietnamese communities

Abstract

Extended spectrum β-lactamase (ESBL)-producing Escherichia coli have been found in healthy individuals in Indonesia and Vietnam. The ISEcp1-bla CTX-M transposition unit of ESBL-producing bacterial isolates has been considered responsible for the production of CTX-M type ESBL and it is important for the dissemination of bla CTX-M . This study aimed to characterize the upstream genetic structure (UGS) of E. coli isolates possessing bla CTX-M-1 group and/or bla CTX-M-9 group genes obtained from healthy individuals in Indonesia and Vietnam. A total of 501 CTX-M type ESBL-producing E. coli isolates possessing bla CTX-M-1 group and/or bla CTX-M-9 group genes were obtained from healthy individuals of the two countries in 2018. The UGSs of the ISEcp1-bla CTX-M transposition unit of the 501 ESBL-producing E. coli isolates were amplified by barcode-adaptor-ligationmediated PCR and analyzed using the Nanopore sequencer. The obtained sequence information was used to classify the UGSs of the ISEcp1-bla CTX-M transposition unit. From the 501 ESBL-producing E. coli isolates, 502 UGSs were obtained, which were classified into 85 UGS types based on the sequence. ISEcp1 of 359 (71.5%) of the 502 UGSs was disrupted by gene insertion, and ISEcp1bla CTX-M transposition unit of most (87.1%) of the determined UGSs was confirmed as plasmidic. Only 6 (7.1%) of the 85 UGS types were common to both countries. Our results indicated that many different UGSs of ISEcp1-bla CTX-M transposition units were detected in Indonesia and Vietnam; hence, we suggest that structurally different kinds of plasmids harboring bla CTX-M were separately distributed in the two countries.

Genomic Characteristics of Extended Spectrum β-Lactamase Producing Escherichia coli Isolates Recovered from a District Hospital in China

“… 38 Recent research has revealed that the CTX-M-1 group and CTX-M-9 group predominate in E. coli isolates with bla CTX-M found in Indonesia and Vietnam. 42 Both CTX-M-15 and CTX-M-55 belong to CTX-M-1 group. CTX-M-55 is derived from CTX-M-15 with only one amino acid substitution (Ala-77-Val) and exhibits greater anti-ceftazidime activity than CTX-M-15.…”

“…ISEcp1 is a member of the IS1380 insertion sequence family, which has been identified as one of several factors involved in the mobilization of bla CTX-M genes and it is important for the dissemination of bla CTX-M . 42 Recent study suggests that the molecular nature of ISEcp1 might be a factor contributing to the high detection rates of E. coli with bla CTX-M-14 in both clinical and community settings. 53 There are a few limitations to our study.…”