Yoghurt as a milk fermentation product has been popular for several years because of its probiotic properties. Probiotic yoghurt is processed by the heated milk then added with lactic acid bacteria and allowed to ferment. However, the series of processes makes some of the nutritional content of milk lost. Therefore, probiotic yoghurt needs to replace this nutritional loss and improve the yoghurt quality. The addition of herbs or natural ingredients could be an effective way to improve the functional properties of yoghurt, such as the addition of Podang Urang mango extract (Mangifera indica L.). This research aimed to recognize the physicochemical, and amino acid profiles of probiotic yoghurt through the addition of Podang Urang mango extract. The addition of Podang Urang mango extracts into yoghurt has significantly affected titratable acid (TA), viscosity, moisture, ash, protein, fat, and lactic acid bacteria (LAB). However, it did not significantly affect water activity and pH. The highest levels of amino acids in yoghurt with the addition of Podang Urang mango extract were leucine and lysine. There was a significant difference in alanine, arginine, tyrosine, proline, and histidine in the percentage of Podang Urang mango extract, while the other amino acids showed insignificant differences.